RESUMO
A possibility to detect antiadenoviral antibodies by surface plasmon resonance (SPR) was demonstrated. Immobilization on the surface of a sensor of the hexone or degraded purified adenovirus of one of the types (Ad 2) allows finding antibodies to the hexone antigenic determinants of wide specificity common for human adenoviruses of different types. Optimum conditions for immobilization of the antigen and formation of the complex antigen-antibody are determined. The comparative assays of the levels of antibodies in rabbit antisera obtained to the hexone and adenoviruses of different types (1, 2 and 6) by SPR and ELISA was analyzed. The biosensor was used to detect antiadenoviral antibodies in the blood sera of children with aggravation of chronic nonspecific broncho-pulmonary diseases. The sensitivity of SPR in comparison with ELISA was 86.9%, in comparison with the method of fluorescing antibodies (MFA)--89.5%.
Assuntos
Adenovírus Humanos/imunologia , Anticorpos Antivirais/isolamento & purificação , Complexo Antígeno-Anticorpo/análise , Técnicas Biossensoriais/métodos , Ressonância de Plasmônio de Superfície , Adenovírus Humanos/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Complexo Antígeno-Anticorpo/sangue , Complexo Antígeno-Anticorpo/imunologia , Antígenos Virais/imunologia , Proteínas do Capsídeo/imunologia , Criança , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Humanos , Doença Pulmonar Obstrutiva Crônica/sangue , Doença Pulmonar Obstrutiva Crônica/virologia , Coelhos , Sensibilidade e EspecificidadeRESUMO
The thesis about importance of specific immunoglobulin preparations in treatment of infectious diseases of viral and microbial etiology, uncontrollable infections, caused by representatives of herpes viruses family in particular, is developed in the paper. The types of immunoglobulin preparations, their advantages, technology peculiarities has been considered; the experience of application, including that of the authors have been analyzed. The necessity and expedience of intensive development of this branch of immunobiotechnology in Ukraine is proved, especially in connection with a high level of infectious morbidity in this country, concrete recommendations are given.
Assuntos
Imunoglobulinas/uso terapêutico , Infecções/terapia , Viroses/terapia , Biotecnologia , Infecções por Herpesviridae/terapia , Humanos , UcrâniaRESUMO
Immunoenzyme assay (non-direct ELISA) was used for analysis of sera from children (age: 4-14 years) with chronic bronchopulmonary diseases. Antibodies to adenoviruses were revealed in 46% of sera as well as a 3-5 time increase of specific antibodies in 50% of cases. Peroxidase-labelled protein A staphylococcus aureus was effective for detection of immune complexes.
