RESUMO
Members of the genus Bartonella (formerly Rochalimaea) were virtually unknown to modern-day clinicians and microbiologists until they were associated with opportunistic infections in AIDS patients about 6 years ago. Since that time, Bartonella species have been associated with cat scratch disease, bacillary angiomatosis, and a variety of other disease syndromes. Clinical presentation of infection with Bartonella ranges from a relatively mild lymphadenopathy with few other symptoms, seen in cat scratch disease, to life-threatening systemic disease in the immunocompromised patient. In some individuals, infection manifests as lesions that exhibit proliferation of endothelial cells and neovascularization, a pathogenic process unique to this genus of bacteria. As the spectrum of disease attributed to Bartonella is further defined, the need for reliable laboratory methods to diagnose infections caused by these unique organisms also increases. A brief summary of the clinical presentations associated with Bartonella infections is presented, and the current status of laboratory diagnosis and identification of these organisms is reviewed.
Assuntos
Infecções por Bartonella/etiologia , Bartonella/patogenicidade , Animais , Bartonella/classificação , Bartonella/ultraestrutura , Infecções por Bartonella/diagnóstico , Infecções por Bartonella/epidemiologia , Doença da Arranhadura de Gato/complicações , Doença da Arranhadura de Gato/etiologia , Gatos , Humanos , Hospedeiro Imunocomprometido , Biologia Molecular , Reação em Cadeia da PolimeraseRESUMO
Revisamos os aspirados medulares de 71 casos de leucemias agudas, em crianças e adultos, diagnosticados nos últimos três anos (1983-85) no Hospital Universitário Walter Cantídio e os reclassificamos segundo as recomendaçöes da Classificaçäo FAB. 81,7% dos casos revistos foram diagnosticados pelo autor enquanto 18,3% obtiveram os laudos iniciais de outros hematologistas. A reprodutibilidade de Classificaçäo FAB é analisada. Obtivemos pelo critério morfológico uma concordância de 87,3% para os dois grandes grupos (LMA e LLA) e de 63,4% ao analisarmos os diferentes subtipos. Quando comparado com o estudo citoquímico a concordância foi de 83,8%. As limitaçöes da morfologia e citoquímica, além dos resultados discrepantes, säo discutidos
Assuntos
Criança , Adulto , Humanos , Leucemia/classificaçãoRESUMO
The second human subcutaneous infection due to Exophiala spinifera from the United States is described. Identification was based on the morphology of the fungus in tissue and the morphological features of the culture obtained from the biopsy material. Correct diagnosis and treatment with ketoconazole and 5-fluorocytosine cured the infection.
Assuntos
Fungos Mitospóricos/isolamento & purificação , Micoses/etiologia , Flucitosina/uso terapêutico , Humanos , Cetoconazol/uso terapêutico , Pessoa de Meia-Idade , Micoses/tratamento farmacológico , Micoses/patologia , Pele/patologia , Especificidade da EspécieRESUMO
Decisions as to the disposition of swine carcasses with lesions attributable to a mycobacterial infection are based upon a lesion criterion which is used as a diagnostic test by federal meat inspectors. Using this criterion, a federal meat inspector classified 58 of 100 pigs as "passed for cooking" and the other 42 pigs passed. Of the 58 pigs passed for cooking and the 42 pigs passed, mycobacterial isolations were made from the lymph nodes of 33 and 15 of the animals, respectively. The lesion criterion as a diagnostic test has the following attributes: 70% sensitive; 53% specific; 23% index of performance; 57% positive accuracy; and 67% negative accuracy.
