RESUMO
Nerve stem cells have a unique characteristic in that they form spherical aggregates, also termed neurospheres, in vitro. The study demonstrated the successful derivation of these neurospheres from bone marrow culture. Their plasticity as nerve stem cells was confirmed. The findings further strengthens the pluripotency of cell populations within the bone marrow.
Assuntos
Células-Tronco Adultas/citologia , Células-Tronco Multipotentes/citologia , Medula Espinal/citologia , Animais , Astrócitos/citologia , Biomarcadores , Medula Óssea/fisiologia , Agregação Celular , Diferenciação Celular/fisiologia , Movimento Celular/fisiologia , Sobrevivência Celular/fisiologia , Oligodendroglia , Projetos Piloto , Ratos , Transplante de Células-TroncoRESUMO
Bone marrow derived Mesenchymal stem cells (MSCs) were evaluated as an alternative source for tissue engineering of peripheral nerves. Human MSCs were subjected to a series of treatment with a reducing agent, retinoic acid and a combination of trophic factors. This treated MSCs differentiated into Schwann cells were characterized in vitro via flow cytometry analysis and immunocytochemically. In contrast to untreated MSCs, differentiated MSCs expressed Schwann cell markers in vitro, as we confirmed by flow cytometry analysis and immunocytochemically. These results suggest that human MSCs can be induced to be a substitute for Schwann cells that may be applied for nerve regeneration since it is difficult to grow Schwann cells in vitro.
Assuntos
Transplante de Medula Óssea , Medula Óssea/patologia , Transplante de Células-Tronco Mesenquimais , Regeneração Nervosa/fisiologia , Tubo Neural/fisiologia , Nervos Periféricos/patologia , Células de Schwann/transplante , Engenharia Tecidual , Técnicas de Cultura de Células , Diferenciação Celular , Citometria de Fluxo , Humanos , Imuno-Histoquímica , TretinoínaRESUMO
Spinal cord, sciatic nerve, olfactory ensheathing cell and bone marrow derived mesenchymal stem cells were evaluated as an alternative source for tissue engineering of nerve conduit. All cell sources were cultured in alpha-MEM medium. Olfactory Ensheathing Cell (OEC) showed the best result with higher growth kinetic compared to the others. Spinal cord and sciatic nerve were positive for GFAP, OEC were positive for GFAP, S100b and anti-cytokeratin 18 but negative for anti-Human Fibroblast.
Assuntos
Células-Tronco Mesenquimais/fisiologia , Animais , Sobrevivência Celular , Feminino , Humanos , Células-Tronco Mesenquimais/citologia , Mucosa Olfatória/citologia , Ratos , Ratos Sprague-Dawley , Nervo Isquiático/citologia , Medula Espinal/citologia , Engenharia TecidualRESUMO
Bone marrow derived progenitor cells have been widely studied for its multipotent property and have proofed to be an important resource in regenerative medicine. However, the propagation of murine bone marrow appeared to be a great challenge as compared to other mammalian species. In this study, various isolation techniques and the plasticity of the isolated cells were evaluated. Our result shows that magnetic sorting technique yielded the most viable cells and displayed wider differentiation capacity.
