RESUMO
Dietary counselling has been identified as one of the nutritional strategies to alleviate cardiometabolic health conditions. Its effectiveness however may vary due to factors such as intensity level and provider while this has not been comprehensively studied. This systematic review and meta-analysis aimed to assess the effects of dietary counselling on the cardiometabolic health in middle-aged and older adults and the sub-group analyses with dietary counselling intensity and the provider were also assessed. Four databases including PubMed, CINAHL Plus with Full Text, Cochrane Library and EMBASE were systematically searched. Data from 22 randomised controlled trials (RCTs) were compiled and those from 9 RCTs were utilised for meta-analysis. Dietary counselling lowered total cholesterol (TC) and fasting blood sugar (FBS) but had no impact on triglycerides (TG) and low-density lipoprotein (LDL). Sub-group analysis revealed significant lowering effect of high intensity dietary counselling for TG (weighted mean difference (WMD): -0.24 mmol/L, 95% confidence intervals (CIs): -0.40 to -0.09), TC (WMD: -0.31 mmol/L, 95% CIs: -0.49 to -0.13), LDL (WMD: -0.39 mmol/L, 95% CIs: -0.61 to -0.16) and FBS (WMD: -0.69 mmol/L, 95% CIs: -0.99 to -0.40) while medium or low intensity dietary counselling did not show favouring effects. Counselling provider showed differential responses on cardiometabolic health between dietitian and all other groups. The findings from this systematic review and meta-analysis suggest that dietary counselling is a beneficial dietary strategy to improve cardiometabolic health in middle-aged and older adults with the emphasis on the counselling intensity.
Assuntos
Doenças Cardiovasculares/prevenção & controle , Aconselhamento/métodos , Dieta Saudável/métodos , Promoção da Saúde/métodos , Doenças Metabólicas/prevenção & controle , Idoso , Aconselhamento/estatística & dados numéricos , Feminino , Promoção da Saúde/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
It was demonstrated in our studies that norfloxacin, a representative member of quinolone antibiotics, can indeed stabilize the gyrase-DNA complex formed during enzymatic cycle. In addition, the formation of the drug-induced complex has been firstly visualized through our atomic force microscopic examination.
Assuntos
DNA Girase/metabolismo , Microscopia de Força Atômica , Quinolinas/farmacologia , Antibacterianos/farmacologia , Eletroforese em Gel de Ágar , Estabilidade Enzimática/efeitos dos fármacos , Estrutura Molecular , Norfloxacino/farmacologiaRESUMO
It has been demonstrated in our studies that the intrinsic curvature of DNA can be easily interrupted by low concentrations of chloroquine and ethidium bromide. In addition, the changes of DNA curvature caused by varying the concentration of these two DNA intercalators can be readily verified through using an atomic force microscope.
Assuntos
DNA/ultraestrutura , Plasmídeos/ultraestrutura , DNA/química , Etídio/química , Microscopia de Força Atômica , Plasmídeos/químicaRESUMO
It is demonstrated that the right and left handedness of DNA supercoils can be engineered precisely and readily at the molecular level in vitro through utilization of the invading property of peptide nucleic acid.
Assuntos
DNA Circular/metabolismo , Ácidos Nucleicos Peptídicos/metabolismo , DNA Circular/química , Engenharia Genética , Microscopia de Força Atômica , Ácidos Nucleicos Peptídicos/química , Plasmídeos/química , Plasmídeos/metabolismoRESUMO
It is demonstrated that the shapes and magnitudes of DNA writhe can be precisely manipulated solely through maneuvering the nucleotide sequence of DNA and without the assistance of topoisomerases.
Assuntos
DNA/química , DNA/genética , Conformação de Ácido Nucleico , Bacteriófago T4 , Sequência de Bases , DNA/síntese química , Estudos de ViabilidadeRESUMO
Synthesis of a fluorescein-tagged circular structure of i-motif was accomplished during our investigations. The backbone circularity of this molecular probe and the presence of the fluorescent moiety within its structure were also confirmed through exonuclease hydrolysis and fluorescence spectroscopy.
Assuntos
Fluoresceína/química , Sondas Moleculares/química , Sondas Moleculares/síntese química , Oligonucleotídeos/síntese química , Pareamento de Bases , Sequência de Bases , Ensaio de Desvio de Mobilidade Eletroforética , Concentração de Íons de Hidrogênio , Hidrólise , Microinjeções , Sondas Moleculares/genética , Neoplasias/metabolismo , Neoplasias/patologia , Oligonucleotídeos/química , Oligonucleotídeos/genética , Oligonucleotídeos/metabolismo , Proteínas/metabolismo , Espectrometria de FluorescênciaRESUMO
A series of oligonucleotides with various lengths that contain nick and topoisomerase I-binding sites were designed. The interactions between these oligonucleotides and human topoisomerase I were investigated and the most efficient one among them has displayed IC(50) value of 6.3 nM. Our studies have also demonstrated that the position of the nick as well as the length of the oligonucleotides were crucial factors for the inhibition of this nuclear enzyme.
Assuntos
Inibidores Enzimáticos/farmacologia , Oligonucleotídeos/química , Inibidores da Topoisomerase I , Sequência de Bases , Sítios de Ligação , Catálise , Núcleo Celular/enzimologia , DNA/química , Desenho de Fármacos , Inibidores Enzimáticos/química , Humanos , Concentração Inibidora 50 , Modelos Genéticos , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Especificidade por SubstratoRESUMO
It is demonstrated in our investigations that certain G-quadruplex structures formed by human telomeric repeats could perform self-cleaving actions. Our further studies verify that these reactions are site-specific and undergo hydrolytic pathways.
Assuntos
Quadruplex G , Telômero/ultraestrutura , Pareamento Incorreto de Bases , Sequência de Bases , DNA/química , Humanos , Hidrólise , Modelos Moleculares , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Ácidos Nucleicos Heteroduplexes/química , Oligonucleotídeos/química , Fosfatos/química , Fatores de TempoRESUMO
Some dumbbell-shaped circular oligonucleotides containing internal C3-spacers and Topo I-binding sites were designed and synthesized which displayed high inhibitory efficiency on the activity of human Topo I as well as resisted the degradation by some DNA repair enzymes.
Assuntos
Inibidores Enzimáticos/farmacologia , Oligonucleotídeos/farmacologia , Inibidores da Topoisomerase I , Sequência de Bases , Sítios de Ligação , Relação Dose-Resposta a Droga , Desenho de Fármacos , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Humanos , Concentração Inibidora 50 , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Oligonucleotídeos/síntese química , Oligonucleotídeos/química , Relação Estrutura-AtividadeRESUMO
It is demonstrated that certain structural assemblies of G-quadruplex are capable of performing selfcleaving actions in a site specific fashion.
