A hybrid catalyst-triggered cascade reaction for cholesterol detection using a smartphone-based miniature fluorescent apparatus.

A new hybrid biological-chemical catalyst, magnetic nanoparticles functionalized with cholesterol oxidase (Fe3O4/APTES/ChOx), was developed for cholesterol detection. In the presence of cholesterol, the enzyme produced H2O2, which facilitated the generation of fluorescent molecules from the fluorogenic substrate with the assistance of Fe3O4 nanoparticles. A smartphone camera with a miniature fluorescent apparatus was used to assess fluorescence emission. Then, a smartphone application was employed to translate the fluorescence intensity to the red, green, and blue (RGB) domain. The developed approach achieved excellent selectivity and acceptable performances while supporting an onsite analysis approach. The practical operational range spanned from 5 to 100 nM, with a detection limit of 0.85 nM. Fe3O4/APTES/ChOx was applied for up to four replicates of reuse and demonstrated stability for at least 30 days. The applicability of the method was evaluated in milk samples, and the results were in accordance with the reference method.

A portable fluorescence detection device based on a smartphone employing carbon nanodots for Mn2+ sensing.

The measurement of fluorescence emission for quantitative analysis is typically based on a traditional spectrofluorometer, which limits an onsite detection approach. Thus, an alternative device should be developed for fulfilling this analysis outside of the laboratory. Therefore, a low-cost, portable, and low-energy consumption fluorescence reader-based smartphone device was developed. An ultraviolet light-emitting diodes (UV-LED) was used to construct the fluorescence device-based smartphone as a low-power excitation light source. The smartphone camera was used as a detector for detecting photons from the fluorescence emission process of the fluorescence probe and was connected to a digital image platform. Transparent acrylic with orange and yellow colors was employed as a filter for reducing the interference from light source intensity. The obtained digital image was converted to red, green and blue (RGB) intensity using a custom-designed smartphone application. N,S-doped carbon nanodots (N,S-CDs) were demonstrated to be a good fluorescence indicator for determining trace quantities of Mn2+ in cosmetics. The approach exhibited high selectivity and sensitivity, detecting and quantifying analytes at 1-5 µM concentrations. Furthermore, the method's detection limit of 0.5 µM reflects its capacity to detect trace amounts of a target analyte. Mn2+ in cosmetic products was successfully analyzed using this device with high accuracy comparable with the results from inductively coupled plasma-optical emission spectroscopy (ICP-OES).

Simultaneous preconcentration and fluorescence detection of ATP by a hybrid nanocomposite of magnetic nanoparticles incorporated in mixed metal hydroxide.

A new approach for increasing the sensitivity of adenosine triphosphate (ATP) detection was demonstrated. The assay was based on the synergetic function of a hybrid nanocomposite (MNPs@MMH) composed of magnetic nanoparticles (MNPs) incorporated in a mixed metal hydroxide (MMH). MNPs@MMH can be utilized as an efficient green extractant and peroxidase catalyst. The trace level of ATP in the sample solution was first extracted by the MNPs@MMH hybrid nanocomposite through the ion exchange properties of MMH and adsorbed on the surface of the MNPs@MMH. The concentration of ATP was related to the fluorescence intensity of 2,3-diaminophenazine (DAP) generated from peroxidase-like activity of the MNPs in the presence of H2O2 and o-phenylenediamine (OPD). In the presence of ATP, the active surface of the MNPs was diminished, and the amount of DAP generated was reduced. Thus, the concentration of ATP was related to the degree of fluorescence decrease compared to the fluorescence intensity of the system without ATP. Based on the proposed strategy, a highly sensitive assay for ATP was achieved. This assay exhibited good selectivity for detection of ATP over derivatives and other common anions. The proposed assay allowed the detection of ATP in a concentration range of 2.5-20 µM with a detection limit of 0.41 µM.

A simple strategy to enhance the sensitivity of fluorescent sensor-based CdS quantum dots by using a surfactant for Hg2+ detection.

A simple strategy to enhance the detection sensitivity of fluorescent sensor-based CdS quantum dots (CdS QDs) for the detection of mercury ions (Hg2+) was demonstrated. L-Cysteine-capped CdS QDs (L-Cyst-CdS QDs) were synthesized and utilized as a probe for selective detection of Hg2+. The fluorescence intensity of the L-Cyst-CdS QDs was quenched in the presence of Hg2+. However, the detection sensitivity was unsatisfactory. Upon the addition of sodium dodecyl sulfate (SDS), the fluorescence intensity of L-Cyst-CdS QDs can be effectively enhanced. On the other hand, the fluorescence intensity of the L-Cyst-CdS QDs in the presence of SDS (SDS@L-Cyst-CdS QDs) was able to be dramatically decreased with the addition of Hg2+. Furthermore, the proposed sensor displayed excellent selectivity towards Hg2+ compared to other cations. Under optimized conditions, the proposed sensor could be applied to detect trace amounts of Hg2+ with a limit of detection of approximately 36 nM. The applicability of this sensor was demonstrated by the determination of Hg2+ in real water samples, and the results agreed with those obtained from cold vapor atomic absorption spectrometry (CVAAS).

Highly selective circular dichroism sensor based on d-penicillamine/cysteamine­cadmium sulfide quantum dots for copper (II) ion detection.

A highly selective circular dichroism sensor based on cysteamine-capped cadmium sulfide quantum dots (Cys-CdS QDs) was successfully developed for the determination of Cu2+. Basically, the Cys-CdS QDs are not active in circular dichroism spectroscopy. However, the circular dichroism probe (DPA@Cys-CdS QDs) can be simply generated in situ by mixing achiral Cys-CdS QDs with D-penicillamine. The detection principle was based on measuring the circular dichroism signal change upon the addition of Cu2+. The strong CD signal of the DPA@Cys-CdS QDs dramatically decreased in the presence of Cu2+, while other cations did not result in any spectral changes. In addition, the degree of the CD signal decrease was linearly proportional to the concentration of Cu2+ in the range of 0.50-2.25 µM (r2 = 0.9919) with a detection limit of 0.34 µM. Furthermore, the proposed sensor was applied to detect Cu2+ in drinking water samples with %recovery values of 102-114% and %RSD less than 6%.

Fluorescence chemodosimeter for dopamine based on the inner filter effect of the in situ generation of silver nanoparticles and fluorescent dye.

A new strategy for the sensitive and selective detection of dopamine (DA) was proposed. The chemodosimeter design was based on the measurement of the fluorescent quenching of fluorescein dye caused by the in situ generation of silver nanoparticles (AgNPs). The AgNPs can be simply generated by a reaction between DA and Ag+ in the presence of polymethacrylic acid (PMAA). In addition, the generated AgNPs possess the maximum surface plasmon resonance (SPR) at 440 nm and an increase in the SPR intensity with an increasing DA concentration. Basically, fluorescein dye can emit the fluorescent intensity maximum at 513 nm with excitation at 487 nm. Thus, fluorescent quenching was achieved due to an inner filter effect from the overlap between the excitation spectrum of the fluorescein dye and the SPR spectrum of the generated AgNPs. The degree of fluorescent quenching linearly depends on the number of generated AgNPs that can be directly related to the concentration of DA. The proposed chemodosimeter can be used to detect DA in a working linear concentration range of 1.0-5.0 µM at a detection limit of 10.6 nM. This chemodosimeter was successfully applied to determine DA in a real urine sample and a dopamine injection formulation with satisfactory results.

Circular dichroism sensor based on cadmium sulfide quantum dots for chiral identification and detection of penicillamine.

A new chemical sensor based on the measuring of circular dichroism signal (CD) was fabricated from cysteamine capped cadmium sulfide quantum dots (Cys-CdS QDs). The chiral-thiol molecules, d-penicillamine (DPA) and l-penicillamine (LPA), were used to evaluate potentials of this sensor. Basically, DPA and LPA provide very low CD signals. However, the CD signals of DPA and LPA can be enhanced in the presence of Cys-CdS QDs. The CD spectra of DPA and LPA exhibited a mirror image profile. Parameters affecting the determination of DPA and LPA were thoroughly investigated in details. Under the optimized condition, the CD signals of DPA and LPA displayed a linear relationship with the concentrations of both enantiomers, ranging from 1 to 35 µM. Detection limits of this sensor were 0.49 and 0.74 µM for DPA and LPA, respectively. To demonstrate a potential application of this sensor, the proposed sensor was used to determine DPA and LPA in real urine samples. It was confirmed that the proposed detection technique was reliable and could be utilized in a broad range of applications.

Cysteamine CdS quantum dots decorated with Fe3+ as a fluorescence sensor for the detection of PPi.

A new sensitive and selective fluorescence sensor for the detection of pyrophosphate (PPi) in aqueous media based on the Fe(3+) decorated cysteamine CdS QDs ([Cys-CdS QDs]-Fe(3+)) was proposed. The presence of PPi can induce the fluorescence quenching of [Cys-CdS QDs]-Fe(3+) due to the high formation constants between the phosphate group and Fe(3+). Because the complex between Fe(3+) and PPi acts as an efficient quencher, the concentration of PPi can be evaluated by tracking the degree of fluorescence quenching. The fabricated sensor was optimized to obtain the best sensor selectivity and sensitivity. Under optimal conditions, a linear relationship between the fluorescence response and the concentration of PPi was established in the range of 0.5-10 µM. The limits of detection and quantitation for PPi were found to be 0.11 and 2.78 µM, respectively. Furthermore, the proposed sensor exhibited high selectivity toward PPi relative to other common anions. The proposed sensor was successfully applied to the detection of PPi in urine samples with satisfactory results.