Expression of Heparansulfate Interacting Protein (HIP) in benign prostatic hyperplasia,prostate intraepithelial neoplasia and prostate cancer

Background: Heparansulfate Interacting Protein (HIP) is up-regulated in various human cancer cell lines at both transcript and protein levels. HIP expression is related to the differentiation status and cancer development. Objectives: To determine HIP in benign prostatic hyperplasia, prostatic intraepithelial neoplasia and prostate cancer tissues. Materials and method: Western blot method was used to determine HIP expression in 3 different types of prostate tissue, including 11 prostate cancer samples, 2 benign prostatic hyperplasia samples and 11 prostatic intraepithelial neoplasia samples. Results. HIP was particularly up-regulated in prostate cancer and prostatic intraepithelial neoplasia, indicating that up-regulation of HIP expression may be an early event in tumorgenesis. Conclusion: The expression of HIP was different between cancer, prostatic intraepithelial neoplasia tissue and benign prostatic hyperplasia. HIP may serve as a prognostic marker for prostate carcinoma.

The differences of transcript level of Heparan-sulfate Interacting Protein (HIP) in benign prostatic hyperplasia, prostatic intraepithelial neoplasia and prostate cancer tissues

Background: Heparan-sufate interacting protetin (HIP) has been known to be up-regulated and expressed in various human cancer cell lines at both transcript and protein levels. HIP\u2019s expression was related to the differentiation status and cancer development. Objective: Using a semi-quantitative PCR method to determine HIP transcript levels in benign prostatic hyperplasia (BPH), prostatic intraepithelial neoplasia (PIN) and prostate cancer tissues. Subjects and methods: 30 samples of BPH, 12 samples of high-grade PIN, and 40 samples of prostate cancer were collected from patients at Viet Duc Hospital and Friendship Hospital. Total RNA was extracted from BPH, PIN and prostate cancer tissues; cDNA synthesis by reverse transcript - polymerase chain reaction (RT - PCR); HIP transcript determination using semi-quantitative PCR. Results: There was significant difference in HIP transcript levels. HIP transcript was very highly up-regulated in the prostate cancer tissues. The up-regulation of HIP transcript was lower in PIN, and lowest in BPH. HIP transcript levels in benign samples were 1/2 and 2/3 compared with cancer and PIN samples, respectively (P< 0.05). These indicated that up-regulation of HIP transcript may be an early event in tumorigenesis. Conclusions: Levels of HIP transcript were different between tissues of prostate cancer, PIN, and BPH. HIP may be a marker for pre-cancer of the prostate.

Effects of artesunate in the cobination of other antimalarial drugs on activities of some murine plasma enzymes

Effects of artesunate in the combination of other antimalarial drugs (primaquine, mefloquine or chloroquine) on activities of some murine plasma enzymes were studied: (1) The combination of artesunate with primaquine induced significantly plasma activities of GOT, GPT, LDH, - HBDH, CK and CK- MB in comparison with artesunate alone; (2) The combination of artesunate with mefloquine also induced significantly plasma activities of GOT, GPT, LDH, -HBDH, CK and CK- MB in comparison with artesunate alone; (3) The companation of artesunate with chloroquine reduced slightly plasma activities of GOT, GPT, LDH, -HBDH, CK and CK-MB. The results obtained in the study suggest that the combination of artesunate with primaquine or mefloquine on murine cellular damage is synergistic, while the combination of artesunate with chloroquine is probably antagonistic