Green synthesis of a photocatalyst Ag/TiO2 nanocomposite using Cleistocalyx operculatus leaf extract for degradation of organic dyes.

Green synthesis has emerged as a sustainable approach for the fabrication of nanomaterials in the last few decades. Leaf extracts have been considered low-cost and highly efficient reactants for the synthesis of nanoparticles. In this study, an aqueous extract of Cleistocalyx operculatus leaves was employed as a reductant to synthesize Ag/TiO2 nanocomposites. The morphology, structure, and interface interaction of the Ag/TiO2 nanocomposites were investigated by (i) X-ray diffraction (XRD) to determine the crystallinity, (ii) scanning electron microscopy (SEM) to determine the morphologies, (iii) energy dispersive X-ray spectroscopy (EDX) to determine the elemental composition and distribution, and (iv) diffuse reflectance spectroscopy (DRS) to understand the optical properties. The results showed that Ag nanoparticles (AgNPs) with particle sizes of 20-40 nm homogeneously covered the surface of the TiO2 nanoparticles. The green-synthesized Ag/TiO2 nanocomposite also exhibited an excellent photodegradation ability for Rhodamine B with a removal percentage up to 91.4% after 180 min of photocatalytic reaction.

Antimicrobial actions of benzimidazoles against oral streptococci.

BACKGROUND/AIM: Benzimidazoles, such as lansoprazole and omeprazole, are used extensively as proton-pump inhibitors to control stomach acid secretion and also have antimicrobial actions against Helicobacter pylori. Our objective was to determine whether they are potentially useful antimicrobials against oral bacteria. METHODS: Streptococcus mutans was our main test organism. It was grown in suspension cultures and biofilms. Standard physiologic assays were used to assess inhibitory actions of benzimidazoles. RESULTS: Benzimidazoles inhibited acid production by S. mutans in suspensions or biofilms. In pH-drop experiments, lansoprazole at a level of only 0.025 mm irreversibly inhibited acid production from glycolysis. Cell uptake of lansoprazole was found to be very pH sensitive and occurred mainly at pH values below about 5, indicating that the protonated form was taken up. Lansoprazole inhibition of glycolysis could be blocked by 2-mercaptoethanol, which suggests that disulfide bonds form between benzimidazoles and protein targets. Identified targets for benzimidazole inhibition included the phosphoenolpyruvate : sugar phosphotransferase system, the glycolytic enzymes aldolase, glyceraldehyde-3-phosphate dehydrogenase, and lactic dehydrogenase, and enzymes such as urease and arginine deiminase. Lansoprazole increased proton permeabilities of S. mutans cells but did not inhibit F-ATPases. Although cells in biofilms were somewhat less sensitive to the agents than those in suspensions, biofilm glycolysis was still markedly inhibited by 0.1 mm lansoprazole. Benzimidazoles are bactericidal, and the oral anaerobes Fusobacterium nucleatum and Prevotella intermedia were more sensitive to killing than was S. mutans. CONCLUSION: Benzimidazoles appear to be useful inhibitors of oral bacteria in acid environments such as progressing caries lesions.

Fluoride and organic weak acids as respiration inhibitors for oral streptococci in acidified environments.

Oxygen metabolism (respiration) of Streptococcus mutans GS-5 involving NADH oxidases, mainly of the H(2)O-producing type, was found to be acid sensitive, as was NADH oxidase activity of cell extracts. Respiration of intact cells in acidified media was also highly sensitive to fluoride, with a 50% inhibitory concentration of about 0.02 mM at pH 4. In contrast, NADH oxidases in cell extracts were fluoride insensitive. Fluoride inhibition of respiration of intact cells was related to weak-acid effects leading to enhanced proton permeability of cells, cytoplasmic acidification and resultant acid inhibition of NADH oxidases and glycolysis. Organic weak acids, such as indomethacin and benzoate, were also effective inhibitors. H(2)O(2) production by intact cells of Streptococcus sanguis NCTC 10904, a peroxide producer, was similarly inhibited by fluoride or organic weak acids in acidified media. Thus, weak acids act as respiratory inhibitors for oral streptococci indirectly by acidifying the cytoplasm rather than acting as direct inhibitors of NADH oxidases.