Seroprevalence of Toxocara at Tra Vinh University Hospital in Vietnam.

OBJECTIVE: The study aims to assess the seroprevalence of Toxocariasis and its associated risk factors among individuals attending the outpatient department at Tra Vinh University Hospital, Vietnam, in 2022. SUBJECTS AND METHODS: A cross-sectional survey was conducted among outpatients of Tra Vinh University Hospital. Toxocariasis diagnosis was based on the Enzyme-Linked Immunosorbent Assay (ELISA) performed at the hospital's laboratory department. We assessed the seroprevalence of Toxocariasis and evaluated associated risk factors, including demographics and certain behaviors. RESULTS: Of the 249 participants surveyed, 165 tested positive for Toxocariasis, yielding a seroprevalence of 66.3% (95% CI: 60.4-72.1). Multivariate analysis revealed that age groups up to 30 and 30-60 years had higher odds of Toxocariasis infection, with adjusted odds ratios (aOR) of 2.52 (95% CI: 1.04-6.11) and 3.21 (95% CI: 1.44-7.15) respectively. Additionally, individuals residing in rural areas and those in contact with dogs or cats had increased risks, with aORs of 2.21 (95% CI: 1.21-4.01) and 2.04 (95% CI: 1.10-3.79), respectively. Notably, hand washing before eating emerged as a protective factor against Toxocariasis, presenting an aOR of 0.38 (95% CI: 0.19-0.76). CONCLUSIONS: Our findings underscore a significant seroprevalence (66.3%) of Toxocara spp. among outpatients at Tra Vinh University Hospital. Proactive measures, including hand hygiene before meals and after pet interactions, are advocated. There is a pronounced need for community-level epidemiological surveillance for human Toxocariasis.

Tuberculin skin testing and QuantiFERON™-TB Gold Plus positivity among household contacts in Vietnam.

SETTING: TB infection (TBI) is diagnosed using the technique-dependent tuberculin skin test (TST) or costly, more accurate interferon-gamma release assays. The TST (⩾10 mm) threshold was indicated by previous research among household contacts in Vietnam, but routine implementation with a different tuberculin reagent showed unexpectedly low TST positivity. OBJECTIVE: TST (⩾5 mm and ⩾10 mm) results were compared to QuantiFERON™-TB Gold Plus (QFT) results in household contacts during community campaigns in 2020 and 2021. DESIGN: This was a cross-sectional multi-center implementation study. RESULTS: Among 1,330 household contacts in 2020, we found a TBI prevalence of 38.6% (QFT), similar to TST ⩾5 mm (37.4%) and higher than TST ⩾10 mm (13.1%). QFT+/TST+ was higher for TST ⩾5 mm (20.7%) than TST ⩾10 mm (9.4%). QFT was not discordant with TST ⩾5 mm (McNemar's test = 0.6, P = 0.5) but was discordant with TST ⩾10 mm (McNemar's test = 263.9, P < 0.01). Older age and Southern region increased odds for positive TST ⩾5 mm and QFT with weaker associations for TST ⩾10 mm. Agreement and discordance were similar in 2021 for 1,158 household contacts. CONCLUSION: Tuberculin reagents affect TST positivity rates. High TB burden countries should monitor reliability of TBI diagnosis, including tuberculin potency, cold chain, and TST technique to optimize eligibility for TB preventive treatment.

CONTEXTE: L'infection tuberculeuse (TBI) est diagnostiquée à l'aide du test cutané à la tuberculine (TST), qui dépend de la technique, ou de tests de libération de l'interféron-gamma, coûteux et plus précis. Des recherches antérieures ont indiqué que le TST (⩾10 mm) est généralement utilisé pour diagnostiquer la TB parmi les contacts familiaux au Vietnam ; la mise en œuvre de routine avec un réactif de tuberculine différent a montré une faible positivité inattendue du TST. OBJECTIF: Les résultats du TST (⩾5 mm et ⩾10 mm) ont été comparés aux résultats de QuantiFERON™-TB Gold Plus (QFT) chez les contacts familiaux au cours des campagnes communautaires de 2020 et 2021. MÉTHODE: Il s'agissait d'une étude transversale multicentrique de mise en œuvre. RÉSULTATS: Parmi 1 330 contacts familiaux en 2020, nous avons trouvé une prévalence de TBI de 38,6% (QFT), similaire au TST ⩾5 mm (37,4%) et plus élevée que le TST ⩾10 mm (13,1%). Le QFT+/TST+ était plus élevé pour le TST ⩾5 mm (20,7%) que pour le TST ⩾10 mm (9,4%). Le QFT n'était pas discordant avec le TST ≥5 mm (test de McNemar = 0,6 ; P = 0,5) mais était discordant avec le TST ⩾10 mm (test de McNemar = 263,9 ; P < 0,01). L'âge avancé et la région méridionale augmentaient les probabilités d'un TST positif ⩾5 mm et d'un QFT, avec des associations plus faibles pour un TST ⩾10 mm. La concordance et la discordance étaient similaires en 2021 pour 1 158 contacts familiaux. CONCLUSION: Les réactifs de tuberculine affectent les taux de positivité des TST. Les pays à forte charge de TB doivent surveiller la fiabilité du diagnostic de TBI, y compris la puissance de la tuberculine, la chaîne du froid et la technique du TST afin d'optimiser l'éligibilité au traitement préventif de la TB.

Physical properties of the layered oxypnictide Sr2ScFeAsO3: a Mössbauer study down to 1.7 K.

A polycrystalline sample of Sr2ScFeAsO3 was studied by 57Fe Mössbauer spectroscopy down to 1.7 K. In contrast to the earlier Mössbauer data, the obtained in this work results indicate that Sr2ScFeAsO3 is in paramagnetic state down to 10 K, while the spectra recorded at 4.6 K and 1.7 K show a weak magnetic order of Fe moments in the Fe2As2 layers. Temperature dependences of isomer shift and quadrupole splitting/shift are compared with specific heat and electrical resistivity data from earlier investigations revealing different local Debye temperatures for the Fe2As2 and perovskite-related Sr2ScO3 layers. Finally, a fast decrease of the carrier density was observed below 80 K and this effect seems to be responsible for the absence of superconductivity in the studied compound.

3D characterisation of dry powder inhaler formulations: Developing X-ray micro computed tomography approaches.

Carrier-based dry powder inhaler (DPI) formulations need to be accurately characterised for their particle size distributions, surface roughnesses, fines contents and flow properties. Understanding the micro-structure of the powder formulation is crucial, yet current characterisation methods give incomplete information. Commonly used techniques like laser diffraction (LD) and optical microscopy (OM) are limited due to the assumption of sphericity and can give variable results depending on particle orientation and dispersion. The aim of this work was to develop new three dimensional (3D) powder analytical techniques using X-ray computed tomography (XCT) that could be employed for non-destructive metrology of inhaled formulations. α-lactose monohydrate powders with different characteristics have been analysed, and their size and shape (sphericity/aspect ratio) distributions compared with results from LD and OM. The three techniques were shown to produce comparable size distributions, while the different shape distributions from XCT and OM highlight the difference between 2D and 3D imaging. The effect of micro-structure on flowability was also analysed through 3D measurements of void volume and tap density. This study has demonstrated for the first time that XCT provides an invaluable, non-destructive and analytical approach to obtain number- and volume-based particle size distributions of DPI formulations in 3D space, and for unique 3D characterisation of powder micro-structure.

A robust single-beam optical trap for a gram-scale mechanical oscillator.

Precise optical control of microscopic particles has been mastered over the past three decades, with atoms, molecules and nano-particles now routinely trapped and cooled with extraordinary precision, enabling rapid progress in the study of quantum phenomena. Achieving the same level of control over macroscopic objects is expected to bring further advances in precision measurement, quantum information processing and fundamental tests of quantum mechanics. However, cavity optomechanical systems dominated by radiation pressure - so-called 'optical springs' - are inherently unstable due to the delayed dynamical response of the cavity. Here we demonstrate a fully stable, single-beam optical trap for a gram-scale mechanical oscillator. The interaction of radiation pressure with thermo-optic feedback generates damping that exceeds the mechanical loss by four orders of magnitude. The stability of the resultant spring is robust to changes in laser power and detuning, and allows purely passive self-locking of the cavity. Our results open up a new way of trapping and cooling macroscopic objects for optomechanical experiments.

Effect of dairy production system, breed and co-product handling methods on environmental impacts at farm level.

Six dairy farms with the same on-farm area and milk production were compared. One farm (G-No) used grass as the sole forage for a herd of Normande cows, a dual-purpose breed. Three farms, with Holstein cows, varied forage for the herd from grass only (G-Ho) to low (G/LM-Ho) or high (G/HM-Ho) proportion of maize silage in the total forage area. Finally, two farms based on G/LM-Ho and G/HM-Ho systems aimed to increase omega-3 fatty acids in the winter diets of cows (G/LM/O3-Ho, G/HM/O3-Ho). Allocation methods (biophysical, protein, economic allocation) and system expansion applied for co-product (milk and meat) handling were examined. The impact categories considered were climate change, climate change including the effects of land use and land use change (CC/LULUC), cumulative energy demand, eutrophication, acidification and land occupation. The impacts per kg of fat-and-protein-corrected milk (FPCM) of G-No were highest, followed by those of G-Ho, G/LM-Ho and G/HM-Ho, regardless co-product handling methods and impact categories (except for eutrophication). CC/LULUC per kg FPCM of G/LM/O3-Ho and G/HM/O3-Ho were both 1% and 3% lower than those of G/LM-Ho and G/HM-Ho, respectively, but other impacts were higher. With system expansion, impacts per kg FPCM were lower than when allocation methods were used. Enteric fermentation was the greatest contributor (45-50%) to CC/LULUC, while grass production was the most important contributor to other impacts. The highest CC/LULUC (for G-No) can be explained by (1) G-No having the lowest milk yield/cow (though it produced the most meat) and (2) the fact that grass required more N fertiliser, but had lower yields than silage maize, even though grassland sequestered C. Among Holstein systems, increasing cow productivity by increasing feed intake (including maize silage and supplementing with concentrate) decreased impacts of milk. Reducing replacement rate and age of first calving also decreased impacts of milk. Increasing cow productivity reduced the amount of on-farm area required to produce a given amount of milk. Thus, the "liberated" on-farm area of Holstein systems was used to produce cash crops, and total impacts of these systems were lower than those of G-No (except for eutrophication and land occupation).

Epidemiological features and risk factors of Salmonella gastroenteritis in children resident in Ho Chi Minh City, Vietnam.

Non-typhoidal Salmonella are an important but poorly characterized cause of paediatric diarrhoea in developing countries. We conducted a hospital-based case-control study in children aged <5 years in Ho Chi Minh City to define the epidemiology and examine risk factors associated with Salmonella diarrhoeal infections. From 1419 diarrhoea cases and 571 controls enrolled between 2009 and 2010, 77 (5∙4%) diarrhoea cases were stool culture-positive for non-typhoidal Salmonella. Salmonella patients were more likely to be younger than controls (median age 10 and 12 months, respectively) [odds ratio (OR) 0∙97; 95% confidence interval (CI) 0∙94-0∙99], to report a recent diarrhoeal contact (8∙1% cases, 1∙8% controls; OR 5∙98, 95% CI 1∙8-20∙4) and to live in a household with >2 children (cases 20∙8%, controls 10∙2%; OR 2∙32, 95% CI 1∙2-4∙7). Our findings indicate that Salmonella are an important cause of paediatric gastroenteritis in this setting and we suggest that transmission may occur through direct human contact in the home.

Effect of farming practices for greenhouse gas mitigation and subsequent alternative land use on environmental impacts of beef cattle production systems.

This study evaluated effects of farming practice scenarios aiming to reduce greenhouse gas (GHG) emissions and subsequent alternative land use on environmental impacts of a beef cattle production system using the life cycle assessment approach. The baseline scenario includes a standard cow-calf herd with finishing heifers based on grazing, and a standard bull-fattening herd using a diet mainly based on maize silage, corresponding to current farm characteristics and management by beef farmers in France. Alternative scenarios were developed with changes in farming practices. Some scenarios modified grassland management (S1: decreasing mineral N fertiliser on permanent grassland; S2: decreasing grass losses during grazing) or herd management (S3: underfeeding of heifers in winter; S4: fattening female calves instead of being reared at a moderate growth rate; S5: increasing longevity of cows from 7 to 9 years; S6: advancing first calving age from 3 to 2 years). Other scenarios replaced protein sources (S7: partially replacing a protein supplement by lucerne hay for the cow-calf herd; S8: replacing soya bean meal with rapeseed meal for the fattening herd) or increased n-3 fatty acid content using extruded linseed (S9). The combination of compatible scenarios S1, S2, S5, S6 and S8 was also studied (S10). The impacts, such as climate change (CC, not including CO2 emissions/sequestration of land use and land-use change, LULUC), CC/LULUC (including CO2 emissions of LULUC), cumulative energy demand, eutrophication (EP), acidification and land occupation (LO) were expressed per kg of carcass mass and per ha of land occupied. Compared with the baseline, the most promising practice to reduce impacts per kg carcass mass was S10 (all reduced by 13% to 28%), followed by S6 (by 8% to 10%). For other scenarios, impact reduction did not exceed 5%, except for EP (up to 11%) and LO (up to 10%). Effects of changes in farming practices (the scenarios) on environmental impacts varied according to impact category and functional unit. For some scenarios (S2, S4, S6 and S10), permanent grassland area and LO per kg of carcass decreased by 12% to 23% and 9% to 19%, respectively. If the 'excess' permanent grassland was converted to fast-growing conifer forest to sequester carbon in tree and soil biomass, CC/LULUC per kg of carcass could be reduced by 20%, 25%, 27% and 48% for scenarios S2, S4, S6 and S10, respectively. These results illustrate the potential of farming practices and forest as an alternative land use to contribute to short- and mid-term GHG mitigation of beef cattle production systems.

Suspected tuberculosis case detection and referral in private pharmacies in Viet Nam.

SETTINGS: Private pharmacies in Hanoi, Viet Nam. OBJECTIVES: To explore the response of health care providers (HCPs) in private pharmacies to suspected tuberculosis (TB) patients. METHODS: A simulated patient method combined with an interview in 128 randomly selected private pharmacies and 10 private pharmacies near TB hospitals. RESULTS: In the simulated patient method and interview, respectively 59 (46%) and 70 (55%) of HCPs referred the TB suspect to general health care. Only 11 (9%) referred the simulated patient to a TB care facility. Fifty-two (42%) of the HCPs identified suspected TB from a fictitious case described on paper; 34 (27%) were aware that free treatment was provided under the National Tuberculosis Programme (NTP). Knowledge about free NTP treatment predicted a higher rate of direct referrals to TB facilities (OR 5.80, 95%CI 1.88-19.62) and greater ability to identify suspected TB from a fictitious case on paper (OR 5.14, 95%CI 2.36-11.73). Pharmacies with Good Pharmacy Practice (GPP) certification were less likely to refer simulated patients to TB facilities than non-GPP pharmacies (OR 0.10, 95%CI ≤0.01-0.79). CONCLUSIONS: Nearly half of HCPs in private pharmacies do not refer TB suspects, possibly contributing to delays in diagnosis and treatment. Knowledge about free NTP treatment predicted better performance of HCPs.

Heat shock protein 70 membrane expression on fibroblast-like synovial cells derived from synovial tissue of patients with rheumatoid and juvenile idiopathic arthritis.

OBJECTIVE: To screen fibroblast-like synovial cells derived from synovial tissue of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) patients for the membrane expression of the heat shock protein Hsp70. METHODS: We performed flow cytometric (fluorescence-activated cell sorting, or FACS) analysis on fibroblast-like synovial cells of 15 RA patients and three JIA patients to investigate Hsp70 membrane expression. Skin fibroblasts derived from the operation wound (n = 4) and peripheral blood mononuclear cells (PBMC) of seven RA and three JIA patients were also tested. Peripheral blood lymphocytes (PBL) and skin fibroblasts of 10 healthy individuals were used as negative controls. RESULTS: A significantly higher percentage of Hsp70 membrane expression was found on fibroblast-like synovial cells derived from arthritis-affected joints in RA patients (mean 47.7%) when compared with autologous skin fibroblasts (mean 9.5%, p < 0.001) and control skin fibroblasts (mean 5.6%, p < 0.001) or autologous PBL (mean CD45/Hsp70-positive 10.4%, p < 0.001) and control PBL (mean CD45/Hsp70-positive 7.7%, p < 0.001). A high percentage of Hsp70 membrane expression was also observed on fibroblast-like synovial cells derived from three patients with JIA (mean 35.2%) when compared with autologous PBL (mean CD45/Hsp70-positive 10.4%). Synovial cells derived from non-affected joints in a patient with RA who underwent synovectomy for trauma showed low expression of Hsp70 (10.9%). CONCLUSION: Fibroblast-like synovial cells derived from patients with severe course of RA and JIA are strongly positive for membrane-expressed Hsp70.

Analysis of the transcriptional response to Rice Yellow Mottle Virus infection in Oryza sativa indica and japonica cultivars.

Several cDNA libraries were constructed using mRNA isolated from roots, panicles, cell suspensions and leaves of non-stressed Oryza sativa indica (IR64) and japonica (Azucena) plants, from wounded leaves, and from leaves of both cultivars inoculated with Rice Yellow Mottle Virus (RYMV). A total of 5549 cleaned expressed sequence tags (ESTs) were generated from these libraries. They were classified into functional categories on the basis of homology, and analyzed for redundancy within each library. The expression profiles represented by each library revealed great differences between indica and japonica backgrounds. EST frequencies during the early stages of RYMV infection indicated that changes in the expression of genes involved in energy metabolism and photosynthesis are differentially accentuated in susceptible and partially resistant cultivars. Mapping of these ESTs revealed that several co-localize with previously described resistance gene analogs and QTLs (quantitative trait loci).