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1.
J Dig Dis ; 24(11): 630-637, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37823361

RESUMO

OBJECTIVES: Laser lithotripsy under fluoroscopic guidance is difficult to perform and risky due to its invisibility. In this study we aimed to investigate the efficacy and safety of a novel endoscopic auxiliary system (NEAS)-assisted lithotripsy under fluoroscopy for treating difficult common bile duct (CBD) stones. METHODS: Patients with difficult CBD stones who were treated with NEAS-assisted laser lithotripsy (NEAS group) or conventional mechanical lithotripsy (ML) under fluoroscopy (ML group) were retrospectively evaluated. The primary outcome was the complete stone clearance rate and the secondary outcomes included operation time, complications, and medical cost. RESULTS: Seventeen patients were treated with NEAS-assisted laser lithotripsy and 144 patients underwent ML. Using the propensity score matching analysis, 17 pairs of cases treated with NEAS-assisted lithotripsy and ML were included. Patients in the NEAS group showed a higher stone clearance rate than the ML group (94.1% vs 58.8%, P = 0.039), as well as shorter operation time (41.9 min vs 49.4 min, P < 0.001) and lower medical cost (USD 4607 vs USD 5014, P < 0.001). There was no significant difference in the complication rate between the two groups (5.9% vs 17.6%, P = 0.601). CONCLUSION: NEAS-assisted fluoroscopy-guided laser lithotripsy is feasible and safe, which may be a promising technique in fluoroscopy-guided laser lithotripsy for difficult CBD stones.


Assuntos
Cálculos Biliares , Litotripsia , Humanos , Cálculos Biliares/diagnóstico por imagem , Cálculos Biliares/terapia , Cálculos Biliares/etiologia , Projetos Piloto , Estudos Retrospectivos , Resultado do Tratamento , Litotripsia/efeitos adversos , Colangiopancreatografia Retrógrada Endoscópica/métodos , Fluoroscopia
2.
J Dig Dis ; 22(12): 721-726, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34817931

RESUMO

OBJECTIVES: Endoscopic retrograde cholangiopancreatography (ERCP) in patients with Roux-en-Y reconstruction after total gastrectomy is difficult to be performed using routine tools. The aim of this study was to evaluate the feasibility and safety of cap-assisted routine adult colonoscope (CARAC) for ERCP in these patients. METHODS: Sixteen consecutive patients with indications of ERCP who had previously undergone total gastrectomy with Roux-en-Y reconstruction at two tertiary care endoscopy centers were identified. All ERCP procedures were carried out by using CARAC. The success rate of reaching the papilla, biliary cannulation and procedure-related adverse events were analyzed. RESULTS: The papilla was successful reached in 11 (68.8%) of the 16 cases, and biliary cannulation was subsequently reached in eight (72.7%) of the 11 cases. The procedures succeeded in three patients by using a percutaneous-endoscopic rendezvous procedure after failed cannulation. Overall clinical success was achieved in 11 (68.8%) of 16 patients. Procedure-related mild acute pancreatitis was observed in 25.0% (4/16) of the cases and mild cholangitis in 18.8% (3/16). No serious adverse events were reported. CONCLUSIONS: CARAC for therapeutic ERCP is safe and effective in treating patients with Roux-en-Y reconstruction after total gastrectomy.


Assuntos
Colangiopancreatografia Retrógrada Endoscópica , Pancreatite , Doença Aguda , Colonoscópios , Estudos de Viabilidade , Gastrectomia/efeitos adversos , Humanos
3.
J Dig Dis ; 16(1): 43-51, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25323957

RESUMO

OBJECTIVE: Pancreatic acinar cell necrosis and subsequent inflammatory response aggravate acute pancreatitis (AP). Tetraspanin CD9 has been reported to mediate inflammatory signaling by regulating molecular organization at the cell surface. This study aimed to investigate the role of CD9 in caerulein-induced AP (CIP) in mice. METHODS: The expression of CD9 was detected in CIP in mice in vivo and cholecystokinin (CCK)/recombinant mouse tumor necrosis factor (rmTNF)-α induced pancreatic acinar cell death in vitro by quantitative real-time polymerase chain reaction, Western blot and immunofluorescence. The roles of CD9 in pancreatic acinar cell death and inflammatory response were further studied through the deletion of CD9 expression using small interfering RNA (siRNA). RESULTS: CD9 was markedly upregulated in pancreatic tissues in mice during the early onset of CIP and was located mainly at the pancreatic acinar cell surface, which was associated with pancreatic damage. Additionally, incubation with CCK or rmTNF-α directly increased the expression of CD9 in isolated mice pancreatic acinar cells in vitro. The deletion of CD9 expression partially reversed both pancreatic acinar cell death induced by CCK and mRNA levels of proinflammatory cytokines produced by damaged acinar cells. CONCLUSION: These results indicate that increased CD9 expression may be involved in pancreatic injury, possibly via the promotion of cytokine expressions in CIP in mice.


Assuntos
Pancreatite/genética , Tetraspanina 29/genética , Células Acinares/imunologia , Doença Aguda , Animais , Ceruletídeo , Colecistocinina/genética , Citocinas/biossíntese , Feminino , Expressão Gênica , Camundongos , Camundongos Endogâmicos BALB C , Pâncreas/fisiopatologia , Pancreatite/induzido quimicamente , RNA/genética , RNA Interferente Pequeno/genética , Distribuição Aleatória , Transdução de Sinais/genética , Fator de Necrose Tumoral alfa/genética , Regulação para Cima
4.
Neurosci Bull ; 25(2): 87-93, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19290027

RESUMO

OBJECTIVE: Numerous studies have described both motor defects and cognitive impairments in several strains of rodents following 3-nitropropionic acid (3-NP) intoxication. In the present study, we investigated spatial recognition memory in Kunming mice that just recovered from motor defects induced by 3-NP. METHODS: Mouse model was made by systemic subacute 3-NP treatment, and spatial recognition memory was measured through the Y-maze Test, a simple two-trial recognition test. RESULTS: (1) On day 15 following 3-NP treatment, affected Kunming mice did not show motor defects in the Rotarod test and presented normal gait again. (2) In the following Y-maze test after 1h interval, the percentage (90.0%) of mice showing novel arm preference in 3-NP treatment group was significantly higher than the random chance level (50%), although it was only slightly higher than that (83.3%) in control group. On day 45 after 3-NP treatment, mice failed to choose unfamiliar novel arm as first choice, and the same occured in the control group. (3) For both post-intoxicated (on day 15 and day 45 following 3-NP treatment) and control groups, the duration in the novel arm and the frequency of entering it, were longer and higher compared with familiar start and other arms. For these mice that recently recovered from motor defects following 3-NP intoxication, no spatial memory deficits were observed through Y-maze Test. CONCLUSION: Kunming mice used in our assays might possess resistance to cognitive impairment induced by 3-NP, which is consistent with previous findings in Swiss EPM-M1 mice.


Assuntos
Convulsivantes/toxicidade , Transtornos da Memória/etiologia , Atividade Motora/efeitos dos fármacos , Transtornos dos Movimentos/etiologia , Nitrocompostos/toxicidade , Intoxicação , Propionatos/toxicidade , Recuperação de Função Fisiológica/fisiologia , Animais , Comportamento Animal , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos , Intoxicação/complicações , Intoxicação/etiologia , Recuperação de Função Fisiológica/efeitos dos fármacos , Teste de Desempenho do Rota-Rod , Fatores de Tempo
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