A new turn-on fluorescent probe for fast detection of diabetic biomarker beta-hydroxybutyrate in vitro.

Beta-hydroxybutyrate (ß-HB) serve as a valuable diagnostic biomarker for Diabetic Ketoacidosis (DKA). Here, a new Schiff base fluorescent probe T was designed and synthesized to detect ß-HB level in aqueous solution in vitro. The probe T can detect ß-HB sensitively and selectively in DMF solution (5.0 × 10-5 M) among other interfering species (cations, anions, amino acids, biomarkers). The detection limit of probe T for ß-HB was calculated to be 0.154 µM. These results demonstrate that the probe T may provide a convenient method for rapid detection of ß-HB to diagnose diabetic ketoacidosis.