RESUMO
The authors have developed enzyme immunoassay (EIA) for detecting antimeasles antibodies in the serum and compared it to routine methods. Preparation and purification of measles virus antigen used in EIA and optimal conditions of the reaction are described. Results of the routine methods and those of EIA correlated. EIA was used to determine titers of antimeasles antibodies in the sera of vaccinated children. 3-4 years after the vaccination the antibodies were undetectable in the sera of 23% of children.
Assuntos
Anticorpos Antivirais/análise , Vírus do Sarampo/imunologia , Animais , Pré-Escolar , Chlorocebus aethiops , Cobaias , Humanos , Técnicas Imunoenzimáticas , Vírus do Sarampo/ultraestrutura , Métodos , Microscopia Eletrônica , Coelhos , Células VeroRESUMO
Two strains of parotitis virus were isolated from patients with clinical symptoms of the disease in epidemiological screening which was carried out during an outbreak of epidemic parotitis in the village of Koltsovo in 1994. The strains were isolated from the saliva of children aged 7 and 8 years vaccinated with live parotitis vaccine at the age of 1.5 years. Primers for the genome site coding for the gene F terminal and the SH gene (a total of 509 n. p.) were estimated and synthesized and the site was amplified. Electron-microscopic examination of purified virus and Vero cells infected with it and serological tests showed a similarity of the newly isolated virus with the Anders strain of parotitis virus. The Dragun-1 and Dragun-2 strains of parotitis virus have been deposited in the collection of viruses at the Vektor State Research Center of Virology and Biotechnology in the village of Koltsovo, Novosibirsk district.
Assuntos
Vírus da Caxumba/isolamento & purificação , Animais , Criança , Chlorocebus aethiops , Surtos de Doenças , Humanos , Microscopia Eletrônica , Caxumba/epidemiologia , Caxumba/virologia , Vírus da Caxumba/genética , Vírus da Caxumba/ultraestrutura , RNA Viral , Sibéria/epidemiologia , Especificidade da Espécie , Células VeroRESUMO
The possibility of using different strains of parotitis virus (Enders, L-3, Jeryl-Leen) as antigens for enzyme immunoassay (EIA) to titer antibodies in human and animal blood sera is analyzed. Methods for preparation and purification of antigen on the basis of the said parotitis virus strains have been developed. Conditions of EIA were optimized. The sensitivity and specificity of EIA and hemagglutination inhibition test were compared.
