RESUMO
A 21-year-old healthy female suffered from an upper respiratory tract infection and 2 days later developed diplopia, unsteady gait, dysarthria and a profound disturbance of consciousness with rapid development of coma. Brain MRI and Tc99m brain perfusion SPECT, EEG, neurophysiological tests and CSF analysis results were unspecific. The detection of serum anti-GQ1b IgG autoantibodies at high titre led to the diagnosis of Bickerstaff's brainstem encephalitis (BBE). Clinical symptoms resolved after treatment with plasma exchange and the outcome was good. Brain MRI was normal, and Tc99m brain perfusion SPECT demonstrated hypoperfusion of the whole cerebral hemispheres and basal ganglia with relative sparing of the thalami and the brainstem. Similar to brain MRI, the sensitivity of Tc99m brain perfusion SPECT in detecting brainstem lesions in typical BBE patients seems to be low.
Assuntos
Tronco Encefálico/diagnóstico por imagem , Tronco Encefálico/patologia , Encefalite , Compostos de Organotecnécio , Tomografia Computadorizada de Emissão de Fóton Único/métodos , Encefalite/complicações , Encefalite/imunologia , Encefalite/patologia , Encefalite/terapia , Feminino , Gangliosídeos/imunologia , Humanos , Imageamento por Ressonância Magnética , Troca Plasmática/métodos , Adulto JovemRESUMO
The aim of the present study is to identify the range of neurological disorders expressing antineuronal antibodies, evaluate the number of different patterns of reactivity that can be detected, and analyse the contribution of these studies to the identification of subgroups of patients. The records of 882 patients were reviewed and their sera and cerebrospinal fluids tested for antineuronal antibodies. Patients were initially divided into four groups according to suspected clinical diagnosis. Autoantibodies were detected by immunohistochemistry, Western blot of gradient-separated neuronal and recombinant proteins and by RIA. Cerebellar degeneration and sensory neuropathies were the most common neurological disorders in which paraneoplastic-related anti-neuronal antibodies were detected. However, in addition to PCA1/anti-Yo and ANNA1/anti-Hu antibodies, we found other reactivities in six patients with cerebellar degeneration: anti-GAD in three females and atypical in the other cases. The widest range of different anti-neuronal antibodies was detected in patients with peripheral sensory neuropathy. Few patients with Stiff-Person syndrome, temporal lobe epilepsy and myoclonus harboured anti-GAD antibodies. Atypical antibodies were detected in single cases with motor neuron disorder and multiple system atrophy. No anti-neuronal antibodies were detected in patients with neurological complications of connective tissue disorders other than Sjögren's syndrome, or in neurological diseases other than motor neuron disease and multiple system atrophy. Our study shows that the spectrum of neurological disorders in which anti-neuronal antibodies can be detected is wider than previously thought. In addition, we found patterns of neuronal staining and Western blot reactivity that differed from those so far reported. This may permit identification of subgroups of patients in whom strategies directed at removing and/or suppressing antibody production could be of some benefit.
Assuntos
Autoanticorpos/imunologia , Doenças do Sistema Nervoso/imunologia , Neurônios/imunologia , Anticorpos Antineoplásicos/metabolismo , Western Blotting/métodos , Proteínas de Ligação a DNA/imunologia , Proteínas de Ligação a DNA/metabolismo , Proteínas ELAV , Feminino , Glutamato Descarboxilase/metabolismo , Humanos , Imuno-Histoquímica/métodos , Masculino , Proteínas de Neoplasias/imunologia , Proteínas de Neoplasias/metabolismo , Proteínas do Tecido Nervoso , Doenças do Sistema Nervoso/classificação , Proteínas de Ligação a RNA/imunologia , Proteínas de Ligação a RNA/metabolismo , Radioimunoensaio/métodosRESUMO
Ifosfamide is an alkylating agent with well-demonstrated efficacy against a large number of malignant diseases. With cyclophosphamide it shares a toxicity profile characterized by myelosuppression and urotoxicity, but ifosfamide has additionally disclosed adverse neurological effects. Ifosfamide-related central nervous system toxicity is characterized by metabolic encephalopathy of varying severity. Symptoms have been reported in 5-30% of all patients treated with ifosfamide. The mechanism of ifosfamide-related central nervous system toxicity has not been fully elucidated, although the symptoms have most often been noted when the drug is given at high doses or administered orally. The neurotoxicity is generally self-limiting and reversible between 48 and 72 h after discontinuation of ifosfamide, although fatal sequelae have been reported. Therapeutic options are now available.
Assuntos
Acetaldeído/análogos & derivados , Antineoplásicos Alquilantes/efeitos adversos , Sistema Nervoso Central/efeitos dos fármacos , Ifosfamida/efeitos adversos , Síndromes Neurotóxicas/etiologia , Acetaldeído/metabolismo , Animais , Encéfalo/efeitos dos fármacos , Carbocisteína/metabolismo , Glutaratos/urina , Glutationa/metabolismo , HumanosRESUMO
We report a direct DNA sequencing analysis of the MECP2 gene undertaken on a further 64 Italian patients with Rett syndrome by using a LICOR 4200 Automated Sequencer. All of the girls entering the study had a consistent clinical diagnosis for this disorder. All coding regions and the flanking intronic splice site sequences were amplified as three non-overlapping fragments by using both forward and reverse primers. The results were then compared to the MECP2 reference sequences published in GenBank. Mutations of the MECP2 gene were identified in 64 of 75 (85.33%) unrelated sporadic Rett syndrome girls. Genotype/phenotype correlation studies, in particular in groups of patients with the same mutation, did not offer definitive and interesting data.
Assuntos
Proteínas Cromossômicas não Histona , Análise Mutacional de DNA , Proteínas de Ligação a DNA/genética , Testes Genéticos , Mutação/genética , Proteínas Repressoras , Síndrome de Rett/genética , Adolescente , Adulto , Sequência de Bases , Criança , Pré-Escolar , Códon sem Sentido/genética , DNA/genética , Feminino , Mutação da Fase de Leitura/genética , Genótipo , Humanos , Itália , Proteína 2 de Ligação a Metil-CpG , Mutação de Sentido Incorreto/genética , Fenótipo , Síndrome de Rett/fisiopatologiaRESUMO
Rett Syndrome (RTT) is an X-linked dominant neurodevelopmental disorder, which almost exclusively affects girls, with an estimated prevalence of one in 10,000-15,000 female births. Mutations in the methyl CpG binding protein 2 gene (MECP2) have been identified in roughly 75% of classical Rett girls. The vast majority of Rett cases (99%) are sporadic in origin, and are due to de novo mutations. We collected DNA samples from 50 Italian classical Rett girls, and screened the MECP2 coding region for mutations by denaturing high-performance liquid chromatography (DHPLC) and subsequent direct sequencing. DHPLC is a recently developed method for mutation screening which identifies heteroduplexes formed in DNA samples containing mismatches between wild type and mutant DNA strands, combining high sensitivity, reduced cost per run, and high throughput. In our series, 19 different de novo MECP2 mutations, eight of which were previously unreported, were found in 35 out of 50 Rett girls (70%). Seven recurrent mutations were characterized in a total of 22 unrelated cases. Initial DHPLC screening allowed the identification of 17 out of 19 different mutations (90%); after optimal conditions were established, this figure increased to 100%, with all recurrent MECP2 mutations generating a characteristic chromatographic profile. Detailed clinical data were available for 27 out of 35 mutation carrying Rett girls. Milder disease was detectable in patients carrying nonsense mutation as compared to patients carrying missense mutations, although this difference was not statistically significant (P = 0.077).
Assuntos
Proteínas Cromossômicas não Histona , Análise Mutacional de DNA/métodos , Proteínas de Ligação a DNA/genética , Mutação/genética , Proteínas Repressoras , Síndrome de Rett/genética , Cromatografia Líquida de Alta Pressão , Códon sem Sentido/genética , Éxons/genética , Feminino , Genes Dominantes/genética , Testes Genéticos , Genótipo , Humanos , Itália , Proteína 2 de Ligação a Metil-CpG , Dados de Sequência Molecular , Mutação de Sentido Incorreto/genética , Desnaturação de Ácido Nucleico , Fenótipo , Polimorfismo de Nucleotídeo Único/genética , Síndrome de Rett/fisiopatologia , Razão de MasculinidadeRESUMO
Cancer is often associated with paraneoplastic syndromes, which may be misinterpreted. We report a case of a patient with occult small cell lung cancer that was initially compounded by clinical features of a paraneoplastic neurologic syndrome. The presence of antineuronal antibodies and positron emission tomography scan guided the search for the underlying tumor. Following chemo-radiotherapy the patient showed no evidence of disease for the next 18 months, whereas only a slight improvement in the neurologic disorders was observed. The course of the small cell lung cancer was very indolent and the paraneoplastic neurologic syndrome did not worsen with the use of cisplatin.
Assuntos
Carcinoma de Células Pequenas/complicações , Carcinoma de Células Pequenas/diagnóstico , Neoplasias Pulmonares/complicações , Neoplasias Pulmonares/diagnóstico , Neoplasias Primárias Desconhecidas/complicações , Neoplasias Primárias Desconhecidas/diagnóstico , Doenças do Sistema Nervoso/etiologia , Síndromes Paraneoplásicas/etiologia , Ataxia/etiologia , Autoanticorpos/metabolismo , Carcinoma de Células Pequenas/imunologia , Tontura/etiologia , Encefalomielite/etiologia , Humanos , Neoplasias Pulmonares/imunologia , Masculino , Pessoa de Meia-Idade , Neoplasias Primárias Desconhecidas/imunologia , Doenças do Sistema Nervoso/imunologia , Neurônios/imunologia , Nistagmo Patológico/etiologia , Síndromes Paraneoplásicas/imunologia , Incontinência Urinária/etiologiaRESUMO
Rett syndrome (RTT) was first described in 1966. Its biological and genetic foundations were not clear until recently when Amir et al reported that mutations in the MECP2 gene were detected in around 50% of RTT patients. In this study, we have screened the MECP2 gene for mutations in our RTT material, including nine familial cases (19 Rett girls) and 59 sporadic cases. A total of 27 sporadic RTT patients were found to have mutations in the MECP2 gene, but no mutations were identified in our RTT families. In order to address the possibility of further X chromosomal or autosomal genetic factors in RTT, we evaluated six candidate genes for RTT selected on clinical, pathological, and genetic grounds: UBE1 (human ubiquitin activating enzyme E1, located in chromosome Xp11.23), UBE2I (ubiquitin conjugating enzyme E2I, homologous to yeast UBC9, chromosome 16p13.3), GdX (ubiquitin-like protein, chromosome Xq28), SOX3 (SRY related HMG box gene 3, chromosome Xq26-q27), GABRA3 (gamma-aminobutyric acid type A receptor alpha3 subunit, chromosome Xq28), and CDR2 (cerebellar degeneration related autoantigen 2, chromosome 16p12-p13.1). No mutations were detected in the coding regions of these six genes in 10 affected subjects and, therefore, alterations in the amino acid sequences of the encoded proteins can be excluded as having a causative role in RTT. Furthermore, gene expression of MECP2, GdX, GABRA3, and L1CAM (L1 cell adhesion molecule) was also investigated by in situ hybridisation. No gross differences were observed in neurones of several brain regions between normal controls and Rett patients.
Assuntos
Proteínas Cromossômicas não Histona , Proteínas de Ligação a DNA/genética , Proteínas Repressoras , Síndrome de Rett/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Análise Mutacional de DNA , Feminino , Testes Genéticos , Proteínas de Grupo de Alta Mobilidade/genética , Humanos , Lactente , Complexo Antígeno L1 Leucocitário , Ligases/genética , Masculino , Glicoproteínas de Membrana/genética , Proteína 2 de Ligação a Metil-CpG , Pessoa de Meia-Idade , Moléculas de Adesão de Célula Nervosa/genética , Receptores de GABA-A/genética , Fatores de Transcrição SOXB1 , Fatores de Transcrição , Enzimas Ativadoras de Ubiquitina , Ubiquitina-Proteína LigasesRESUMO
We recently mapped a locus for a new variant of autosomal dominant myopathy (Swedish families) with proximal muscle weakness, early respiratory muscle involvement, and unique muscle biopsy findings to chromosomal region 2q24-31. In this study, a French family with a similar clinical phenotype and pathology (muscle biopsy) was investigated to see whether the disease gene associated with the myopathy is mapped to the same region as the one in the Swedish families; however, chromosomal region 2q24-q31 was completely excluded. In order to localise the disease gene for the French family, a genome-wide scan was performed using polymorphic microsatellite markers. A maximum two-point lod score of 2.11 (the highest lod score that can be achieved in this family) was obtained for the markers in the region between D2S1272 and D2S1260, spanning 4 cM. This result suggests that the gene responsible for the French form is likely to be located on chromosome 2q21.
Assuntos
Genes Dominantes , Debilidade Muscular/genética , Doenças Musculares/genética , Músculos Respiratórios/patologia , Mapeamento Cromossômico , Cromossomos Humanos Par 2/genética , DNA/genética , Saúde da Família , Feminino , França , Ligação Genética , Genótipo , Haplótipos , Humanos , Escore Lod , Masculino , Debilidade Muscular/patologia , Doenças Musculares/patologia , LinhagemRESUMO
Two Swedish families with autosomal dominant myopathy, who also had proximal weakness, early respiratory failure, and characteristic cytoplasmic bodies in the affected muscle biopsies, were screened for linkage by means of the human genome screening set (Cooperative Human Linkage Center Human Screening Set/Weber version 6). Most chromosome regions were completely excluded by linkage analysis (LOD score <-2). Linkage to the chromosomal region 2q24-q31 was established. A maximum combined two-point LOD score of 4.87 at a recombination fraction of 0 was obtained with marker D2S1245. Haplotype analysis indicated that the gene responsible for the disease is likely to be located in the 17-cM region between markers D2S2384 and D2S364. The affected individuals from these two families share an identical haplotype, which suggests a common origin.
Assuntos
Cromossomos Humanos Par 2/genética , Doenças Musculares/genética , Feminino , Testes Genéticos , Genótipo , Humanos , Escore Lod , Masculino , Repetições de Microssatélites , Debilidade Muscular/genética , Linhagem , Fenótipo , Insuficiência Respiratória/genética , Músculos Respiratórios/anormalidadesRESUMO
We report the pathological findings of a woman with a sub-acute cerebellar syndrome who had undergone surgery 3 years before for endometrial carcinoma. Both serum and cerebrospinal fluid contained high titres of autoantibodies against the cytoplasm of Purkinje cells that recognized a band of 62 kDa on immunoblotting of neuronal extracted proteins (pattern anti-Yo). No tumour was found despite a full range of gynaecological investigations; the neoplastic marker CA125 was slightly elevated and oligoclonal bands were detected in the cerebrospinal fluid. The patient died from acute myocardial infarction 4 months after developing this syndrome. At autopsy, no macroscopic evidence of tumour was obtained and the brain showed no abnormalities. On microscopic examination of the central nervous system diffuse degeneration of Purkinje cells could be seen throughout the cerebellum. Immunohistochemical analysis showed a CD8 lymphocyte infiltration in the cerebellum and cerebral cortex and diffuse microglial activation throughout the brain. These cells expressed high levels of MHC-II antigens on their cell membranes. The serum autoantibodies reacted with the cytoplasm of the remaining Purkinje cells. The short interval between the onset of symptoms and death of the patient could explain the difference between our findings and those reported in the literature in which no inflammatory infiltrates were detected. The immunohistochemical findings as well as the inflammatory cerebrospinal fluid profile seen in our case seem to support the concept that in paraneoplastic cerebellar degeneration with anti-Yo antibodies, an immune mediated mechanism is responsible for the damage to the cerebellum.
Assuntos
Autoantígenos/análise , Doenças Cerebelares/imunologia , Proteínas de Ligação a DNA/imunologia , Proteínas de Neoplasias/imunologia , Proteínas do Tecido Nervoso , Idoso , Western Blotting , Linfócitos T CD8-Positivos , Doenças Cerebelares/patologia , Evolução Fatal , Feminino , Antígenos HLA/análise , Humanos , Imuno-Histoquímica , Células de Purkinje/química , Células de Purkinje/patologiaRESUMO
Anti-neuron-specific autoantibodies are widely recognised as useful, though non-specific, diagnostic markers of paraneoplastic neurological disorders. However, controversies on the best way to detect these autoantibodies have recently arisen, and the use of different procedures for their detection by different laboratories has made results difficult to compare. The aim of this study was to adapt the existing immunohistochemical techniques used for the detection of anit-neuron autoantibodies to improve their visualisation and to facilitate a wide application of these procedures. Sera and cerebrospinal fluid (CSF) were obtained from 15 patients known to carry paraneoplastic anti-neuronal autoantibodies; in addition, one serum with "atypical" anti-neuron autoantibody and 18 control sera were studied. Paraformaldehyde-fixed, paraffin-embedded rat nervous tissue and formalin-fixed, paraffin-embedded human nervous tissue treated in a microwave oven were used as substrate; the reactions were developed by immunoperoxidase methods. At the dilutions used for diagnostic purposes, all the sera and CSFs showed staining whose intensity and specificity was comparable to that obtained using frozen tissue; the end-point dilutions were, however, reduced. The atypical pattern of staining of one serum was confirmed and better emphasised using these procedures; all control sera and CSFs were negative. The morphology was improved by the use of paraffin-embedded tissues; moreover, the results obtained are permanent because of peroxidase staining, which makes it possible to use them as standards for further investigations and for comparison between different laboratories. The convenience of using paraffin-embedded material could facilitate a wide application of these procedures in clinical neurology.
Assuntos
Autoanticorpos/análise , Encéfalo/metabolismo , Síndromes Paraneoplásicas/metabolismo , Animais , Cerebelo/metabolismo , Gânglios Espinais/metabolismo , Humanos , Imuno-Histoquímica , Ratos , Ratos Sprague-DawleyRESUMO
An X-ray processing system by computer is described. The scanning device and display are illustrated. The main processing methods are analyzed, in particular the linear and non linear ones. An example of image processing which is the base for future work is shown.