Exogenous long-term treatment with 17ß-oestradiol alters the innervation pattern in pig ovary.

The aim of the present study was to determine the effect of long-term 17ß-oestradiol (E2) exposure, a simulation of pathological states that occur with oestrogen overproduction, on the innervation patterns of ovaries in adult gilts. The intraovarian distribution and density of nerve fibres immunoreactive (IR) to protein gene product (PGP) 9.5 and containing dopamine-ß-hydroxylase (DBH), neuropeptide Y (NPY), somatostatin (SOM) and galanin (GAL) were determined. From Day 4 of the first oestrous cycle to Day 20 of the second cycle studied, experimental gilts were injected with E2 (1000µg every 12h) whereas control gilts were injected with corn oil. After E2 administration, there was an increase in the number of PGP9.5-, DBH-, NPY- and GAL-IR fibres. Numerous PGP9.5-IR terminals were observed within the ground plexus around secondary follicles and small or medium tertiary follicles. Long-term E2 treatment increased the density of DBH- and NPY-IR fibres in the cortical part of the ground plexus, DBH- and GAL-IR fibres in the medullary part of the ground plexus, DBH-IR fibres near small and medium tertiary follicles and NPY-IR fibres around medullary arteries. The data indicate that long-term exposure of gilts to E2 increases the total number of intraovarian fibres, including sympathetic fibres. These results suggest that elevated E2 levels that occur during pathological states may affect the innervation patterns of ovaries and their function(s).

Immunohistochemical characterization of the porcine nodose ganglion.

This study reports for the first time the presence of several biologically active substances in the nodose ganglion of immature female pigs. The expression and distribution pattern of the studied substances was examined using a double-labeling immunofluorescence technique. In order to visualize the entire population of the ganglionic cell bodies, PGP 9.5, the pan-neuronal marker was used. The distribution and relative proportion of immunolocalized substance P, calcitonin gene related peptide, neuronal isoform of nitric oxide synthase and galanin in perikarya were evaluated. Quantitative analysis of the nodose ganglion neurons revealed that 16.187% (±1.22) of the immunoreactive PGP 9.5 was localized in the perikarya of the left-side ganglion whereas 14.234% (±3.63) of the right-side ganglion neurons expressed substance P, respectively. Accordingly, the proportion of the perikarya with calcitonin gene related peptide ranged from 12.667% (±2.66) for the left ganglion compared with 14.875% (±2.33) for the right one. With regard to the neuronal isoform of the nitric oxide synthase expression, our study revealed a population of 18.703% (±2.50) in the left and 13.336% (±1.25) in the right ganglion, respectively. The immunoreactivity for galanin was found in a relatively small population of neurons of the left nodose ganglion, where galanin-immunoreactive perikarya constituted 1.163% (±1.26), while in the contralateral ganglion 0.865% (±0.32) of total perikarya. No nerve fibers immunopositive for the above studied substances were encountered.