RESUMO
This study was designed to explore the role played by ambiguity in the experience of creepiness, as well as the relevance of personality traits for predicting individual differences in susceptibility to getting "creeped out," In an online study, a mixed sample of 278 college undergraduates and adults (60 males, 206 females, 12 nonbinary or chose not to report; Mean age = 31.43, range 18-68) recruited through social network platforms filled out scales measuring their tolerance for ambiguity and their susceptibility to having "Not Just Right Experiences." They then rated 25 images (12 normal, 13 prejudged to be creepy or confusing) on creepiness and several other adjective dimensions. The findings indicated that individuals who were less tolerant of ambiguity and those highly susceptible to not just right experiences perceived ambiguous or creepy persons, places, and objects to be more creepy, confusing and disturbing. Both measures were negatively related to time spent looking at confusing or creepy images, and females were generally more easily creeped out by creepy and confusing images than were males. The results support the conclusion that current models of creepiness are correct; the emotional experience of getting "creeped out" does indeed appear to be triggered by the need to resolve ambiguity.
RESUMO
BACKGROUND: ExCyto PCR cells provide a novel and cost effective means to amplify DNA transformed into competent bacterial cells. ExCyto PCR uses host E. coli with a chromosomally integrated gene encoding a thermostable DNA polymerase to accomplish robust, hot-start PCR amplification of cloned sequences without addition of exogenous enzyme. RESULTS: Because the thermostable DNA polymerase is stably integrated into the bacterial chromosome, ExCyto cells can be transformed with a single plasmid or complex library, and then the expressed thermostable DNA polymerase can be used for PCR amplification. We demonstrate that ExCyto cells can be used to amplify DNA from different templates, plasmids with different copy numbers, and master mixes left on ice for up to two hours. Further, PCR amplification with ExCyto cells is comparable to amplification using commercial DNA polymerases. The ability to transform a bacterial strain and use the endogenously expressed protein for PCR has not previously been demonstrated. CONCLUSIONS: ExCyto PCR reduces pipetting and greatly increases throughput for screening EST, genomic, BAC, cDNA, or SNP libraries. This technique is also more economical than traditional PCR and thus broadly useful to scientists who utilize analysis of cloned DNAs in their research.
