[Express diagnostics of endotoxaemia during postoperative period in cardiac surgical patients].

Medical technologies development and recent approaches in management of patients with septic complications during the early postoperative period present new obstacles to the laboratory service. Endotoxin is a main agent in the systemic inflammatory cascade and plays important role in sepsis pathogenesis. Recent express methods of diagnostics allow determining blood activity of endotoxin during 30-50 min. 55 ICU patients with clinical and laboratory signs of systemic inflammatory response syndrome (SIRS) after cardiac surgery were studied in the single-center prospective research. Endotoxaemia was diagnosed by express tests. Level of endotoxaemia was assessed before and after sorption in 15 patients receiving complex intensive care with selective lipopolysaccharide (LPS) adsorption. Endotoxaemia level assessment allows to define indications for different programs of intensive care in time and to assess its efficiency. Test systems for assessment endotoxin level based on the principle of interaction antigen - antibody (EAA and MACH-endotox spp.) is a most efficient for express diagnostics of endotoxaemia.

[Effect of 0.2--0.3M sodium chloride solutions on the properties of the chromatin of lymphocytes in different stages os maturation]. / Vliianie 0.2--0.3 M rastvorov khloristogo natriia na svoistva khromatina limfotsitov, nakhodiashchikhsia na raznykh stadiiakh sozrevaniia.

The response of lymph node lymphocytes stimulated to proliferation at different stages of maturation by cell seeding at the high cell density was studied. The binding of acridine orange (A3) and 3H-actinomycin D (3H-AMD) to the cell nuclei was used as a criterion of the functional state of lymphocyte chromatin. The "crowding" stimulation of the lymph node lumphocytes resulted in the increase of AO and 3H-AMD binding by the cells of different sizes, i.e. at different stages of maturation. The degree of binding differs in cells of different size and the increase is expressed to a greater extent in small lymphocytes. The cell treatment with 0.2--0.3M sodium chloride solutions eliminates the differences in AO and 3H-AMD binding between the activated an non-activated lymphocytes of all sizes what appears to be due to the removal of some weakly bound non-histone proteins from the activated cells. The differences in AO and 3H-AMD binding between the cells of different sizes are retained after this treatment. Hence, the proteins removed from the chromatin under the effect of such a treatment are not responsible for the chromatin changes accompanying the cell maturation.

[A quantitative cytochemical study of mouse hepatocyte chromatin in the early periods following hepatectomy]. / Kolichestvennoe tsitokhimicheskoe issledovanie khromatina gepatotsitov myshi v rannie sroki posle chastichnoi gepatéktomii

Mouse hepatocytes of different ploidy react to the partial hepatectomy by changes in cytochemical properties of the chromatin, leading to an increased binding to DNA of a basic dye acridine orange and an antibiotic actinomycin D, and to decreased DNP stability to acid hydrolysis. These alterations reach their maximum 1.5 and 2.5 hours after the operation in di-and tetraploid, and octaploid cells, resp. and decrease markedly by 5 hours after the operation. The differences in the chromatin reaction of cells with different ploidy to the activation stimulus reflects presumably the particular roles played by these cells in liver regeneration. The difference in chromatin cytochemical properties of activated and non-activated hepatocytes is interpreted as a result of changes in DNA interaction with chromatin proteins.