Applicability of universal Bacteroidales genetic marker for microbial monitoring of drinking water sources in comparison to conventional indicators.

Water quality monitoring is essential for the provision of safe drinking water. In this study, we compared a selection of fecal indicators with universal Bacteroidales genetic marker to identify fecal pollution of a variety of drinking water sources. A total of 60 samples were collected from water sources. The microbiological parameters included total coliforms, fecal coliforms, Escherichia coli and fecal streptococci as the fecal indicator bacteria (FIB), Clostridium perfringens and H2S bacteria as alternative indicators, universal Bacteroidales genetic marker as a promising alternative fecal indicator, and Salmonella spp., Shigella spp., and E. coli O157 as pathogenic bacteria. From 60 samples analyzed, Bacteroidales was the most frequently detected indicator followed by total coliforms. However, the Bacteroidales assay failed to detect the marker in nine samples positive for FIB and other alternative indicators. The results of our study showed that the absence of Bacteroidales is not necessarily an evidence of fecal and pathogenic bacteria absence and may be unable to ensure the safety of the water. Further research, however, is required for a better understanding of the use of a Bacteroidales genetic marker as an indicator in water quality monitoring programs.

Ultraviolet irradiation: An effective inactivation method of Aspergillus spp. in water for the control of waterborne nosocomial aspergillosis.

Invasive aspergillosis is the second most common cause of nosocomial fungal infections and occurring mainly by Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger. There is evidence that nosocomial aspergillosis may be waterborne. This study was conducted to evaluate the ultraviolet (UV) irradiation efficiency in terms of inactivating the most important Aspergillus species in water since these are potential sources for nosocomial aspergillosis. A continuous flow UV reactor which could be used as a point-of-use (POU) system was used to survey Aspergillus inactivation by UV irradiation. The inactivation efficiency of UV fluence (4.15-25 mJ/cm(2)) was measured by determination of fungal density in water before and after radiation. Because turbidity and iron concentration are two major water quality factors impacting UV disinfection effectiveness, the potential influence of these factors on UV inactivation of Aspergillus spp. was also measured. The 4 log inactivation for A. fumigatus, A. niger and A. flavus at a density of 1000 cfu/ml was achieved at UV fluences of 12.45 mJ/cm(2), 16.6 mJ/cm(2) and 20.75 mJ/cm(2), respectively. The inactivation efficiency for lower density (100 cfu/ml) was the same as for the higher density except for A. flavus. The removal efficiency of Aspergillus spp. was decreased by increasing the turbidity and iron concentration. UV disinfection could effectively inactivate Aspergillus spores from water and eliminate potential exposure of high-risk patients to fungal aerosols by installation of POU UV systems.

Predictive indicators of the safety of swimming pool waters.

Many outbreaks related to swimming pools could have been prevented or reduced if the pool had been well managed and effectively monitored. The aim of this study was to evaluate physicochemical and microbial parameters that can be proposed as an indicator for the safety of swimming pools. A total of 234 water samples, over a 10-month period in 2006-2007, were collected from indoor swimming pools in Isfahan. All water samples were analyzed for physicochemical and microbial parameters including temperature, pH, turbidity, conductivity, free chlorine, heterotrophic plate count (HPC), total (TC) and fecal coliforms (FC), fecal streptococci (FS) Staphylococcus aureus (Sa) and Pseudomonas aeruginosa (Pa). The highest isolation of microbial indicators was for total coliforms (38%) and the lowest for fecal streptococci (3%). The correlation analyses indicated that free chlorine concentration had a significant negative relationship with the heterotrophic bacteria population and total coliforms. Total coliforms presented a significant correlation with the other microbiological indicators. The results clearly showed that the hygienic quality of the swimming pools was dependent on the efficacy of disinfection. Thus, the free chlorine and pH were good operational indices for the quality control of swimming pools and must be maintained in the recommended range to ensure optimal disinfection. The results also showed that TC and HPC were reliable and practical indicators for routine quality surveillance and assessment of the efficiency of the disinfection process and safety of swimming pools.