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1.
Hum Mol Genet ; 25(6): 1116-28, 2016 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-26769677

RESUMO

Familial dysautonomia (FD) is an autosomal recessive neurodegenerative disease that affects the development and survival of sensory and autonomic neurons. FD is caused by an mRNA splicing mutation in intron 20 of the IKBKAP gene that results in a tissue-specific skipping of exon 20 and a corresponding reduction of the inhibitor of kappaB kinase complex-associated protein (IKAP), also known as Elongator complex protein 1. To date, several promising therapeutic candidates for FD have been identified that target the underlying mRNA splicing defect, and increase functional IKAP protein. Despite these remarkable advances in drug discovery for FD, we lacked a phenotypic mouse model in which we could manipulate IKBKAP mRNA splicing to evaluate potential efficacy. We have, therefore, engineered a new mouse model that, for the first time, will permit to evaluate the phenotypic effects of splicing modulators and provide a crucial platform for preclinical testing of new therapies. This new mouse model, TgFD9; Ikbkap(Δ20/flox) was created by introducing the complete human IKBKAP transgene with the major FD splice mutation (TgFD9) into a mouse that expresses extremely low levels of endogenous Ikbkap (Ikbkap(Δ20/flox)). The TgFD9; Ikbkap(Δ20/flox) mouse recapitulates many phenotypic features of the human disease, including reduced growth rate, reduced number of fungiform papillae, spinal abnormalities, and sensory and sympathetic impairments, and recreates the same tissue-specific mis-splicing defect seen in FD patients. This is the first mouse model that can be used to evaluate in vivo the therapeutic effect of increasing IKAP levels by correcting the underlying FD splicing defect.


Assuntos
Modelos Animais de Doenças , Disautonomia Familiar/metabolismo , Disautonomia Familiar/patologia , Processamento Alternativo , Animais , Vias Autônomas/metabolismo , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Disautonomia Familiar/genética , Éxons , Humanos , Peptídeos e Proteínas de Sinalização Intracelular , Íntrons , Masculino , Camundongos , Camundongos Transgênicos , Mutação , Neurônios/metabolismo , Splicing de RNA/genética , RNA Mensageiro/metabolismo , Células Receptoras Sensoriais/metabolismo
2.
PLoS One ; 5(11): e13890, 2010 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-21085480

RESUMO

BACKGROUND: Neural crest stem cells (NCSCs) are a transient multipotent embryonic cell population that represents a defining characteristic of vertebrates. The neural crest (NC) gives rise to many derivatives including the neurons and glia of the sensory and autonomic ganglia of the peripheral nervous system, enteric neurons and glia, melanocytes, and the cartilaginous, bony and connective tissue of the craniofacial skeleton, cephalic neuroendocrine organs, and some heart vessels. METHODOLOGY/PRINCIPAL FINDINGS: We present evidence that neural crest (NC) competence can be acquired very early when human embryonic stem cells (hESCs) are selectively neuralized towards dorsal neuroepithelium in the absence of feeder cells in fully defined conditions. When hESC-derived neurospheres are plated on fibronectin, some cells emigrate onto the substrate. These early migratory Neural Crest Stem Cells (emNCSCs) uniformly upregulate Sox10 and vimentin, downregulate N-cadherin, and remodel F-actin, consistent with a transition from neuroepithelium to a mesenchymal NC cell. Over 13% of emNCSCs upregulate CD73, a marker of mesenchymal lineage characteristic of cephalic NC and connexin 43, found on early migratory NC cells. We demonstrated that emNCSCs give rise in vitro to all NC lineages, are multipotent on clonal level, and appropriately respond to developmental factors. We suggest that human emNCSC resemble cephalic NC described in model organisms. Ex vivo emNCSCs can differentiate into neurons in Ret.k(-) mouse embryonic gut tissue cultures and transplanted emNCSCs incorporate into NC-derived structures but not CNS tissues in chick embryos. CONCLUSIONS/SIGNIFICANCE: These findings will provide a framework for further studying early human NC development including the epithelial to mesenchymal transition during NC delamination.


Assuntos
Células-Tronco Embrionárias/metabolismo , Células-Tronco Multipotentes/metabolismo , Crista Neural/metabolismo , Células-Tronco Neurais/metabolismo , Animais , Diferenciação Celular/genética , Linhagem da Célula , Movimento Celular/genética , Células Cultivadas , Embrião de Galinha , Análise por Conglomerados , Células-Tronco Embrionárias/citologia , Transição Epitelial-Mesenquimal/genética , Perfilação da Expressão Gênica , Humanos , Imuno-Histoquímica , Mucosa Intestinal/metabolismo , Intestinos/embriologia , Camundongos , Células-Tronco Multipotentes/citologia , Crista Neural/citologia , Células-Tronco Neurais/citologia , Análise de Sequência com Séries de Oligonucleotídeos , Técnicas de Cultura de Órgãos , Fator de Transcrição PAX3 , Fatores de Transcrição Box Pareados/genética , Fatores de Transcrição Box Pareados/metabolismo , Receptor de Fator de Crescimento Neural/genética , Receptor de Fator de Crescimento Neural/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Transcrição SOXE/genética , Fatores de Transcrição SOXE/metabolismo
3.
J Cell Mol Med ; 14(6B): 1476-84, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19799651

RESUMO

Cholinergic neurotransmission is essential for many important functions in the brain, including cognitive mechanisms. Here we demonstrate that human embryonic stem (hES) cells differentiate into a population of neuronal cells that express the cholinergic enzyme choline acetyltransferase and homeobox proteins specifying neuronal progenitors of ventral telencephalic lineage. These differentiated cells express transcripts for cholinergic alpha(3), alpha(4) and alpha(7) nicotinic acetylcholine (ACh) receptor subunits and for M1, M2 and M3 muscarinic acetylcholine receptor (mAChR) subtypes. Stimulation with brain-derived neurotrophic factor, neurotrophin-3, ciliary neurotrophic factor and nerve growth factor increases the proportion of cholinergic neurons. These cholinergic receptors also mediate ACh-evoked increase in cytosolic calcium levels, and this response was unaffected by extracellular calcium removal and was abolished by the mAChR antagonist scopolamine. Our findings demonstrate expression of functional cholinergic receptors on hES cell-derived neurons, which may provide a source of expandable cells to facilitate screening of novel cholinergic drugs and useful for evaluating cell transplantation in animal models of cholinergic dysfunction.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Colina/metabolismo , Células-Tronco Embrionárias/citologia , Fatores de Crescimento Neural/farmacologia , Neurônios/citologia , Biomarcadores/metabolismo , Cálcio/metabolismo , Agregação Celular/efeitos dos fármacos , Linhagem Celular , Células-Tronco Embrionárias/efeitos dos fármacos , Células-Tronco Embrionárias/metabolismo , Humanos , Neuroglia/citologia , Neuroglia/efeitos dos fármacos , Neuroglia/metabolismo , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Prosencéfalo/metabolismo , Receptores Colinérgicos/metabolismo , Transmissão Sináptica/efeitos dos fármacos
4.
Proc Natl Acad Sci U S A ; 104(30): 12506-11, 2007 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-17640880

RESUMO

In a previous study, we found that human neural stem cells (HNSCs) exposed to high concentrations of secreted amyloid-precursor protein (sAPP) in vitro differentiated into mainly astrocytes, suggesting that pathological alterations in APP processing during neurodegenerative conditions such as Alzheimer's disease (AD) may prevent neuronal differentiation of HNSCs. Thus, successful neuroplacement therapy for AD may require regulating APP expression to favorable levels to enhance neuronal differentiation of HNSCs. Phenserine, a recently developed cholinesterase inhibitor (ChEI), has been reported to reduce APP levels in vitro and in vivo. In this study, we found reductions of APP and glial fibrillary acidic protein (GFAP) levels in the hippocampus of APP23 mice after 14 days treatment with (+)-phenserine (25 mg/kg) lacking ChEI activity. No significant change in APP gene expression was detected, suggesting that (+)-phenserine decreases APP levels and reactive astrocytes by posttranscription regulation. HNSCs transplanted into (+)-phenserine-treated APP23 mice followed by an additional 7 days of treatment with (+)-phenserine migrated and differentiated into neurons in the hippocampus and cortex after 6 weeks. Moreover, (+)-phenserine significantly increased neuronal differentiation of implanted HNSCs in hippocampal and cortical regions of APP23 mice and in the CA1 region of control mice. These results indicate that (+)-phenserine reduces APP protein in vivo and increases neuronal differentiation of HNSCs. Combination use of HNSC transplantation and treatment with drugs such as (+)-phenserine that modulate APP levels in the brain may be a useful tool for understanding mechanisms regulating stem cell migration and differentiation during neurodegenerative conditions in AD.


Assuntos
Doença de Alzheimer/patologia , Amiloide/metabolismo , Diferenciação Celular/efeitos dos fármacos , Neurônios/citologia , Fisostigmina/análogos & derivados , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacos , Amiloide/genética , Animais , Células Cultivadas , Regulação da Expressão Gênica , Proteína Glial Fibrilar Ácida/metabolismo , Humanos , Camundongos , Camundongos Transgênicos , Neuroglia/citologia , Neuroglia/metabolismo , Neurônios/efeitos dos fármacos , Fisostigmina/farmacologia , Transplante de Células-Tronco
5.
J Neurosci Res ; 85(3): 504-14, 2007 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-17203487

RESUMO

Retinoic acid (RA) and nerve growth factor (NGF) have multiple functions in the regulation of neuronal development. In the present study, we characterized the expression of different nicotinic acetylcholine receptor (nAChR) subtypes in the cholinergic SN56 cell line and investigated the roles of RA and NGF in the expression of choline acetyltransferase (ChAT) and different nAChR subtypes. The nAChR agonist [(3)H]epibatidine was bound to two sites, with apparent affinities of 13 and 380 pM. RT-PCR analysis revealed expression of alpha3, alpha4, alpha5, alpha7, beta2, and beta4 nAChR subunits. RA treatment induced morphological changes, and the mRNA level of ChAT was maximally elevated after 4 days of exposure. The density of [(3)H]epibatidine binding sites and the mRNA and protein level of the alpha3 and beta2 nAChR subunits were also increased by RA-induced differentiation. RA down-regulated the mRNA and protein level of the alpha4 nAChR subunit, whereas no significant change was observed in the mRNA and protein level of the alpha7 nAChR subunit. NGF treatment increased the mRNA and protein level of the alpha3 and beta2 nAChR subunits. No morphological effects of NGF were observed, and the mRNA level of ChAT and mRNA and protein level of the alpha4 and alpha7 nAChR subunits were not significantly altered. Validation was performed with real-time RT-PCR. The present results show that RA and NGF have different effects on the expression of ChAT and the morphology and the expression pattern of different nAChR subunits in cholinergic SN56 cells.


Assuntos
Colina O-Acetiltransferase/genética , Fator de Crescimento Neural/farmacologia , Receptores Colinérgicos/genética , Receptores Nicotínicos/genética , Tretinoína/farmacologia , Animais , Compostos Bicíclicos Heterocíclicos com Pontes/farmacocinética , Linhagem Celular , Colina O-Acetiltransferase/metabolismo , Primers do DNA , Regulação da Expressão Gênica/efeitos dos fármacos , Cinética , Camundongos , Subunidades Proteicas/genética , Piridinas/farmacocinética , Receptores Nicotínicos/efeitos dos fármacos
6.
Glia ; 55(4): 385-99, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17152062

RESUMO

The great potential of human embryonic stem (hES) cells offers the opportunity both for studying basic developmental processes in vitro as well as for drug screening, modeling diseases, or future cell therapy. Defining protocols for the generation of human neural progenies represents a most important prerequisite. Here, we have used six hES cell lines to evaluate defined conditions for neural differentiation in suspension and adherent culture systems. Our protocol does not require fetal serum, feeder cells, or retinoic acid at any step, to induce neural fate decisions in hES cells. We monitored neurogenesis in differentiating cultures using morphological (including on-line follow up), immunocytochemical, and RT-PCR assays. For each hES cell line, in suspension or adherent culture, the same longitudinal progression of neural differentiation occurs. We showed the dynamic transitions from hES cells to neuroepithelial (NE) cells, to radial glial (RG) cells, and to neurons. Thus, 7 days after neural induction the majority of cells were NE, expressing nestin, Sox1, and Pax6. During neural proliferation and differentiation, NE cells transformed in RG cells, which acquired vimentin, BLBP, GLAST, and GFAP, proliferated and formed radial scaffolds. gamma-Aminobutyric acid (GABA)-positive and glutamate positive neurons, few oligodendrocyte progenitors and astrocytes were formed in our conditions and timing. Our system successfully generates human RG cells and could be an effective source for neuronal replacement, since RG cells predominantly generate neurons and provide them with support and guidance.


Assuntos
Células-Tronco Embrionárias/fisiologia , Células Epiteliais/fisiologia , Neuroglia/fisiologia , Adesão Celular/fisiologia , Diferenciação Celular/fisiologia , Linhagem Celular , Meios de Cultura Livres de Soro , Primers do DNA , Humanos , Imuno-Histoquímica , Proteínas do Tecido Nervoso/biossíntese , RNA/biossíntese , RNA/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa
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