RESUMO
Forty two saliva samples from rabid dogs were examined by intracerebral inoculation of weanling and suckling mice. Although rabies virus assay were successful in all of the samples in both groups of mice used, a significant higher death proportion (p < 0.01) were observed in the suckling mice group.
Assuntos
Doenças do Cão/microbiologia , Vírus da Raiva/patogenicidade , Raiva/diagnóstico , Saliva/microbiologia , Animais , Animais Lactentes , Cães , Camundongos , Vírus da Raiva/isolamento & purificação , Cultura de VírusRESUMO
Rabies virus was isolated from the brain, salivary and interscapular (brown fat) glands, heart, lungs and testis of naturally infected vampire bat Desmodus rotundus found paralyzed in the day at Barueri, São Paulo State. The rabies virus isolations were made by intracerebral inoculation in 4-5 days and 30 days old mice. The virus strain was identified as rabies virus by the Sellers and Faraco (Mann) techniques, the fluorescent antibody test and intracerebral inoculation of mice. The isolation of virus from lungs and testis was made only in suckling mice. Only one of eight and two of eight mice inoculated died with rabies.
Assuntos
Reservatórios de Doenças/veterinária , Vírus da Raiva/isolamento & purificação , Raiva/microbiologia , Ratos/microbiologia , Animais , Encéfalo/microbiologia , Brasil , Coração/microbiologia , Pulmão/microbiologia , Masculino , Raiva/veterinária , Glândulas Salivares/microbiologia , Testículo/microbiologiaRESUMO
Rabies virus was isolated from insectivorous bat Molossus obscurus found in a semi-paralyzed condition, in broad daylight, in Campinas, São Paulo State. Suckling and adult mice inoculated intracerebrally with a 20% suspension of bat brain showed typical rabies symptoms within eight days. The mortality of inoculated mice was 100%. Negri bodies were seen in the brains of infected mice by Sellers and Fraco's methods. Rabies antigens was found in the brains of inoculated mice by fluorescent antibodies test.
Assuntos
Encéfalo/microbiologia , Quirópteros/microbiologia , Vírus da Raiva/isolamento & purificação , Raiva/veterinária , Animais , Brasil , Raiva/microbiologiaRESUMO
Complement-fixation test based in 50% hemolytic end point was applied to investigate the immune status to rabies of dogs vaccinated with heigh egg-passage Flury vaccine. The complement-fixation titer was compared with serum neutralization results. Twenty-five sera was employed and the complement fixation titer varied of 0 to 256. Three sera was anticomplementary. The results indicated a lack of quantitative correlation, but was found a qualitative correlation between the two methods.