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J Vet Med Sci ; 85(4): 443-446, 2023 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-36806247

RESUMO

The geneLEAD VIII is a fully-automated nucleic acid extraction/quantitative PCR equipment developed by Precision System Science Co., Ltd., (PSS). To take advantage of its capability, we developed a quantitative assay system to measure growth of animal viruses. The system was used to assay one of the Chinese herbal extracts whose anti-malarial activities were previously reported and demonstrated its dose-dependent anti-viral activity against feline infectious peritonitis virus (FIPV), a feline coronavirus causing the fatal diseases in cats, and relatively low cell toxicity. The assay developed in this study is useful to screen antiviral drugs and the anti-FIPV activity of the herbal extract identified have a potential to lead to development of new drugs against FIPV and other coronaviruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).


Assuntos
Antineoplásicos , COVID-19 , Doenças do Gato , Coronavirus Felino , Peritonite , Animais , Gatos , Coronavirus Felino/genética , SARS-CoV-2/genética , COVID-19/veterinária , Antivirais/uso terapêutico , Reação em Cadeia da Polimerase/veterinária , Peritonite/veterinária , Teste para COVID-19/veterinária , Doenças do Gato/tratamento farmacológico
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