Discovery of hydrolytic catalysts in a peptidocalixarene library by binding assay with a transition state analogue for the hydrolysis.

Hydrolytic catalysts were found in a peptidocalixarene library by binding assay with a transition state analogue for the hydrolysis. The rate of the reaction can be specifically enhanced up to 50-fold in the presence of the discovered catalyst.

On-bead screening of a library to detect host-guest complexation by an aniline reporter.

We developed a new labeling reagent and a color assay system in water to detect binding between target molecules and library members on beads, which is free of label-induced artifacts that can cause misleading results.