Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Am J Nephrol ; 34(6): 496-504, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22041478

RESUMO

BACKGROUND/AIMS: DNA fragmentation is one of the typical features of apoptosis, frequently induced by oxidative stress. Increased oxidative stress is known to be related to several pathological processes. In this study, we assessed oxidative damage in the early follow-up period after kidney transplantation measuring DNA oxidation and fragmentation of mononuclear cells and the circulating levels of inflammatory cytokines. METHODS: Blood samples from 30 kidney transplant recipients were collected before transplantation and after 2 days, 1 month and 6 months. Oxidative DNA fragmentation was measured by Comet Assay, whereas DNA oxidation was evaluated measuring 8-OHdG leukocyte levels. Serum IL-1ß, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α were assayed using a multiplex ELISA analysis. RESULTS: At 6 months after transplantation, a significant reduction in DNA fragmentation and IL-6 plasma levels was observed; DNA oxidation was higher in patients with a worse outcome, with delayed graft function and low nutritional status. We also found a correlation of IL-6 and IL-10 levels with DNA fragmentation and of IL-10 levels with DNA oxidation. CONCLUSION: Low levels of oxidation and apoptosis at 6 months after transplantation correlate with a better recovery of renal function in kidney allografts. The measurement of cytokine levels confirmed a reduction of inflammatory parameters within 6 months of follow-up.


Assuntos
Transplante de Rim/métodos , Estresse Oxidativo , 8-Hidroxi-2'-Desoxiguanosina , Idoso , Ensaio Cometa , DNA/química , Fragmentação do DNA , Desoxiguanosina/análogos & derivados , Desoxiguanosina/farmacologia , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Sobrevivência de Enxerto , Humanos , Inflamação , Interleucina-10/química , Masculino , Pessoa de Meia-Idade , Oxigênio/química , Fatores de Tempo , Resultado do Tratamento
2.
Pediatr Res ; 70(4): 379-83, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21691251

RESUMO

Preterm infants are exposed to conditions that can impair renal function. We evaluated the ability of serum and urinary neutrophil gelatinase-associated lipocalin (sNGAL and uNGAL) to predict renal function in the first weeks of life. From September 2008 to July 2009, infants weighing ≤1500 g at birth with no major congenital anomalies or sepsis were eligible. We measured sNGAL and uNGAL levels at birth. To evaluate renal function, we determined changes in serum creatinine (sCreat) and estimated GFR (eGFR) from birth to d 21. Forty neonates (mean GA, 27 ± 2 wk) completed the study. Renal function improved in 32 of 40 (80%) infants (normal renal function, NRF group) (sCreat, from 0.97 ± 0.2 to 0.53 ± 0.13 mg/dL; eGFR, from 15.3 ± 4.1 to 28.6 ± 7.9 mL/min), whereas renal function worsened in 8 of 40 (20%) infants (impaired renal function, IRF group) (sCreat, from 0.71 ± 0.27 to 0.98 ± 0.43 mg/dL; eGFR from 23 ± 14.7 to 16.4 ± 9.1 mL/min). The uNGAL/urinary creatinine (uCreat) ratio at birth was higher in the IRF group (31.05 ng/mg) than the NRF group (6.0 ng/mg), and uNGAL was significantly higher in IRF group, detecting IRF with a cutoff of 100 ng/mL. uNGAL levels at birth may have a predictive role in very LBW (VLBW) infants.


Assuntos
Proteínas de Fase Aguda/urina , Biomarcadores/urina , Recém-Nascido/urina , Recém-Nascido Prematuro/urina , Recém-Nascido de muito Baixo Peso/urina , Rim/metabolismo , Lipocalinas/urina , Proteínas Proto-Oncogênicas/urina , Biomarcadores/sangue , Feminino , Idade Gestacional , Humanos , Recém-Nascido/sangue , Recém-Nascido Prematuro/sangue , Recém-Nascido de muito Baixo Peso/sangue , Testes de Função Renal , Lipocalina-2 , Lipocalinas/sangue , Masculino , Estudos Prospectivos , Proteínas Proto-Oncogênicas/sangue , Sensibilidade e Especificidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...