Assuntos
Mpox , Criança , Humanos , Mpox/epidemiologia , Mpox/prevenção & controle , Surtos de DoençasRESUMO
The Deleted in AZoospermia Like (DAZL) gene is expressed in prenatal and postnatal germ cells. In this study, we cloned and characterized the porcine Deleted in AZoospermia Like (pDAZL) gene. We found the full-length coding sequence of the pDAZL encoded a protein of 295 amino acids with a RNA recognition motif (amino acids 41-111) and a DAZ repeat (amino acids 167-120). The deduced protein sequence of pDAZL is 92.5% and 91.5% similar to those of human and bovine, respectively. PCR-MspI-RFLP and PCR-TaqI-RFLP were established to detect an A/G mutation in intron 7 and a C/A mutation in intron 9, respectively. Associations of two SNPs with litter size traits were assessed in Large White (n=275) and DIV (n=128) pig populations, and the statistical analysis demonstrated that CC produced 0.716 more (P<0.05) piglets born alive than CD genotypes in Large White pigs at TaqI locus (C/A mutation in intron 9), and the dominance effect was 0.304 pig per litter (P<0.05). This result suggests that the pDAZL gene might be a good candidate gene of litter size trait and provides some marker information for marker-assisted selection (MAS).
Assuntos
Polimorfismo de Nucleotídeo Único , Proteínas de Ligação a RNA/genética , Sus scrofa/genética , Animais , Clonagem Molecular , Análise Mutacional de DNA , Frequência do Gene , Marcadores Genéticos/genética , Marcadores Genéticos/fisiologia , Tamanho da Ninhada de Vivíparos/genética , Masculino , Proteínas de Ligação a RNA/isolamento & purificação , Reprodução/genéticaRESUMO
The ring finger protein 4 gene (RNF4), which might play a role in fetal germ cell development as well as in oocyte and granulosa cell maturation, was one of the potential candidate genes for reproductive traits. In the present work, we isolated the complete coding sequence of porcine RNF4 gene, identified a single nucleotide polymorphism (SNP: T/C) in intron5, and developed a PCR-SacII-RFLP genotyping assay. Association of this SNP with reproductive traits was assessed in three populations with diverse genetic backgrounds. One was Chinese Qingping sows. Another was consisted of crossbred sows derived from Landrace, Large White, Chinese Tongcheng and/or Chinese Meishan (Line DIV). The third is Large White x Meishan (LW x M) F(2) slaughtered population. Statistical analysis demonstrated that, in the first parity, the difference between RNF4 genotypes and reproductive traits of both Qingping and Line DIV sows was not significant. In the second and subsequent litters, CC animals in Qingping population had more piglets born (+1.74 piglets) and piglets born alive (+2.02 piglets) than sows with the TT genotype (P<0.05). Line DIV sows inheriting the CC genotype had additional 0.69 piglets born compared to the TC animals (P<0.05) in second and subsequent litters. No significant difference was observed between genotypes and reproductive tracts components in F(2) animals. In addition, we found RNF4 gene has a significant additive effect on both piglet born and piglet born alive in Qingping animals (P<0.05). Results here suggested that the RNF4 SNP was significantly associated with litter size in two populations and could be useful in selection for increasing litter size in pigs. Further studies were needed to confirm these preliminary researches.
Assuntos
Proteínas Nucleares/genética , Reprodução/genética , Suínos/genética , Fatores de Transcrição/genética , Dedos de Zinco/genética , Sequência de Aminoácidos , Animais , Animais Recém-Nascidos , Sequência de Bases , Cruzamentos Genéticos , Feminino , Perfilação da Expressão Gênica/veterinária , Genótipo , Tamanho da Ninhada de Vivíparos/genética , Dados de Sequência Molecular , Polimorfismo de Fragmento de Restrição , Polimorfismo de Nucleotídeo Único , RNA/química , RNA/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterináriaRESUMO
A study was carried out between November 1981 and April 1982 on the immunological effect of administering trivalent live, oral polio vaccine to 200 mature healthy neonates from Henan Province, China. The initial dose of vaccine was given at 3 days of age, and 2 months thereafter antibodies to poliovirus types 1, 2, and 3, respectively, were detected in 46.7%, 60.7% and 48.6% of the neonates; after the second dose, the levels were 86.9%, 95.3%, and 97.2%, with geometric mean titres of 1:106.2, 1:349.8, and 1:232.5. Almost 100% of neonates exhibited antibodies after the fourth dose of vaccine. Eighty-two percent of the neonates excreted poliovirus for at least a week after the initial dose of vaccine, and this increased to 99% after the second dose. Seroconversion at 4 months of age was similar to that of a group of controls who received their initial dose of vaccine at 2 months of age; however, immunization of neonates induced immunity to poliovirus at the earliest possible age.
