RESUMO
This paper addresses the distribution and occurrence of harmful organic substances in coal gangue dump from Jiulong Coal Mine and its influence on the environment. The samples were taken from the coal gangue dump and coal waste water stream and analyzed by organic geochemical methods. The results indicate that the coal gangues contain abundant harmful organic substances like polycyclic aromatic hydrocarbons. The TOC and sulfur contents of the samples are much higher than those of the background sample except Sample JL7. The contents of organic bulk parameters are relatively high. Ten carcinogenic PAHs were identified and these harmful organic substances have influenced the surrounding area. Along the waste water stream, organic substances pollute at least 1,800 m far from the coal gangue dump.
Assuntos
Minas de Carvão , Monitoramento Ambiental , Poluentes Ambientais/análise , Resíduos Industriais/análise , Compostos Orgânicos/análise , China , Poluentes Ambientais/química , Poluentes Ambientais/toxicidade , Compostos Orgânicos/toxicidade , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Medição de RiscoRESUMO
This paper describes a culture system which supports the formation of B cell and some T cell colonies under serum-free conditions in peripheral blood samples of normal individuals and patients with chronic lymphocytic leukemia (CLL) of B cell type. In this system, serum is replaced by bovine serum albumin, transferrin, cholesterol, insulin and catalase or horseradish peroxidase. In addition, it is necessary to add staphylococcus protein A, mitomycin-treated T cells as feeders and phytohemagglutinin leukocyte-conditioned medium as a source of growth factors. The plating efficiency is greatly enhanced when normal cells are incubated with galactose oxidase prior to plating and when CLL cells are exposed sequentially to neuraminidase and galactose oxidase.
