In situ generation of electrochemiluminescent DNA nanoflowers as a signal tag for mucin 1 detection based on a strategy of target and mimic target synchronous cycling amplification.

A sensitive electrochemiluminescent (ECL) aptasensor consisting of a novel ECL signal tag of DNA nanoflowers (DNA NFs) and a highly efficient target conversion strategy for the MUC1 assay was developed, which not only increased the stability for luminophore loading, but also greatly improved the detection sensitivity.

[Effects of COR and Me-JA on mineral elements in L. japonica].

ICP-AES technique was used to determine the content, accumulation and proportion of mineral elements in L. japonica and analyze the differences after treatment by COR and Me-JA. The results show that: (1) The content of K, Mg, Na, Zn, B and Si in L. japonica were increased by 5.82%, 2.55%, 155.17%, 5.34%, 16.11% and 142.15% respectively after treatment by COR, while the content of P, Ca, Fe and Mn was reduced by 3.99%, 19.20%, 38.89% and 35.96% (2) After treatment by Me-JA, the content of K, Na, Zn, B, and Si were increased by 4.46%, 137.93%, 6.09%, 10.09% and 89.24%, however the content of P, Ca, Mg, Fe and Mn were decreased by 9.82%, 20.29%, 8.49%, 42.00% and 36.80% respectively. (3) When treated by COR and Me-JA, K:P and Na:Zn were increased, while Ca:Mg and Fe:Mn and B:Si were decreased. The results of this study define the influence of COR and Me-JA on the mineral elements of L. japonica, and provide a scientific basis for the rational use of plant growth regulators as well.