[Retracted] Inhibiting proliferation and migration of lung cancer using small interfering RNA targeting on Aldo­keto reductase family 1 member B10.

Following the publication of this paper, it was drawn to the Editor's attention by a concerned reader that data from the cell adhesion experiment shown in Fig. 11 were strikingly similar to data appearing in different form in Fig. 6B in another article written by different authors at different research institutes [Huang Z, Li J, Du S, Tang Y, Huang L, Xiao L and Tong P: FKBP14 overexpression contributes to osteosarcoma carcinogenesis and indicates poor survival outcome. Oncotarget 7: 39872­39884, 2016].  Owing to the fact that the contentious data in the above article had already been published prior to its submission to Molecular Medicine Reports, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor apologizes to the readership for any inconvenience caused. [Molecular Medicine Reports 17: 2153­2160, 2018; DOI: 10.3892/mmr.2017.8173].

Effect of perioperative nursing for artificial knee replacement on patients with osteosarcoma of the distal femur.

OBJECTIVE: To investigate the clinical application of perioperative nursing care for patients with osteosarcoma of the distal femur who received artificial knee replacement. METHODS: A total of 80 patients with osteosarcoma of the distal femur admitted to our hospital from March 2019 to March 2020 were selected as research subjects and divided into the control group and the study group according to their admission sequence. The control group was given routine nursing care, while the study group was given perioperative nursing care. The negative emotions, sleep quality, limb function, pain, complication rate, and nursing satisfaction of the two groups of patients after nursing care were analyzed. RESULTS: After nursing care, (1) the Hamilton depression rating scale (HAMD) and Hamilton anxiety scale (HAMA) scores of the study group were both lower than those of the control group; (2) the sleep quality score of the study group was lower than that of the control group (P < 0.001); (3) the study group obtained higher limb function score and excellent-and-good rate than the control group (P < 0.05); (4) the pain score of the study group was lower than that of the control group (P < 0.05), and the time to functional exercise and length of hospital stay of the study group were shorter than those of the control group (P < 0.05); (5) patients in the study group had a lower complication rate than those in the control group (P=0.02); (6) the nursing satisfaction rating of the study group was higher than that of the control group (P < 0.001). CONCLUSION: For patients with osteosarcoma of the distal femur treated by artificial knee replacement, in addition to the amelioration of negative emotions and sleep quality, perioperative nursing also improves the patients' limb function and satisfaction and mitigates pain and complications.

Inhibiting proliferation and migration of lung cancer using small interfering RNA targeting on Aldo-keto reductase family 1 member B10.

Lung cancer is the leading cause of global cancer­associated mortality. Genomic alterations in lung cancers have not been widely characterized, however, the molecular mechanism of tumor initiation and progression remain unknown, and no molecularly targeted have been specifically developed for its treatment and diagnosis. The present study observed the upregulation of Aldo­keto reductase family 1 member Bio10 (AKR1B10) lung cancer tissues by analyzing two public lung cancer gene expression datasets. Further experiments in silencing AKR1B10 demonstrated that the expression of AKR1B10 was associated with cell proliferation, cell cycle, adhesion and invasion, as well as extracellular­signal­regulated kinase/mitogen activated protein kinase signal pathway. The overexpression of AKR1B10 in lung cancer indicates the important role of AKR1B10 in tumorigenesis. These findings suggest that AKR1B10 could be a potential diagnosis and treatment mark of lung cancer.

Inhibition of the growth of non-small cell lung cancer by miRNA-1271.

Non-small cell lung cancer (NSCLC) is a common lung cancer of high incidence. Since NSCLCs often grow rapidly, it is critical to elucidate the underlying mechanisms. Previous studies have shown that microRNAs (miRNAs) play critical roles in the carcinogenesis of NSCLC. Specifically, miR-1271 has been shown to downregulate in NSCLC cells. However, whether the downregulation of miR-1271 relates to carcinogenesis of NSCLC is unknown. Here, we analyzed the levels of miR-1271 and mTor in NSCLC specimens and studied their correlation. We performed bioinformatics analysis and luciferase-reporter assay to study the association of miR-1271 with mTor mRNA. We analyzed miR-1271 levels in NSCLC cells and studied their effects on mTor levels. The growth of miR-1271-modified NSCLC cells was evaluated in vitro in an MTT assay, and in vivo by bioluminescence. We detected significantly higher levels of mTor and significantly lower levels of miR-1271 in the NSCLC specimens, compared to the paired non-tumor lung tissues. We found that miR-1271 bound to the 3'UTR of mTor mRNA to prevent its translation. Overexpression of miR-1271 in NSCLC cells decreased mTor protein levels, whereas inhibition of miR-1271 increased mTor protein levels, without affecting mTor transcripts. Moreover, overexpression of miR-1271 suppressed the NSCLC growth in vitro and in vivo, while inhibition of miR-1271 significantly increased NSCLC growth. Together, these data suggest that in NSCLC, miR-1271 may inhibit the cancer cell growth through mTor suppression.

Functional roles of PC-PLC and Cdc20 in the cell cycle, proliferation, and apoptosis.

Phosphatidylcholine-specific phospholipase C (PC-PLC) is the major enzyme in the Phosphatidylcholine (PC) cycle and is involved in many long-term cellular responses such as activation, proliferation, and differentiation events. Cell division cycle 20 homolog (Cdc20) is an essential cell-cycle regulator required for the completion of mitosis. Our previous studies identified the interaction between PC-PLC and Cdc20. Through the interaction, Cdc20 could mediate the degradation of PC-PLC by Cdc20-mediated ubiquitin proteasome pathway (UPP). In this study, we found that PC-PLC might not be involved in cancer metastasis. Inhibition of PC-PLC by D609 could cause cell proliferation inhibition and apoptosis inhibition in CBRH-7919 cells. Inhibition of PC-PLC could also influence the cell cycle by arresting the cells in G1 phase, and Cdc20 might be involved in these processes. Taken together, in this report, we provided new evidence for the functional roles of PC-PLC and Cdc20 in the cell cycle, proliferation, and apoptosis in CBRH-7919 cells.