Effects of PASP/NTA and TS on the phytoremediation of pyrene-nickel contaminated soil by Bidens pilosa L.

Bidens pilosa L. (B. pilosa) is considered as an effective phytoremediation plant. In this study, polyaspartic acid (PASP), aminotriacetic acid (NTA) and tea saponin (TS) was combined with B. pilosa. to strengthen the phytoremediation efficiency. The removal rate of pyrene reached 95.8% with B. pilosa alone. The release of Ni in NTA and NTA-TS treatments was 20-30 times than untreated contaminant soil. The Ni concentration in roots of B. pilosa increased by 63.1% and 58.6% in PASP and PASP-TS treatments, respectively. The Ni concentration in leaves of B. pilosa increased by 55.9% and 186% in NTA and NTA-TS treatments, respectively. The growth of B. pilosa was significantly promoted in PASP and PASP-TS treatments. PASP, NTA and TS significantly promoted soil microbial activities. The results showed that B. pilosa was beneficial to pyrene removal. PASP and NTA had positive effects on absorption of Ni by B. pilosa.

The relief effects of organic acids on Scirpus triqueter L. under pyrene-lead stress.

During phytoremediation of polycyclic aromatic hydrocarbons (PAHs) and heavy metals, the phytoremediation plants are often stressed by pollutants, which would reduce the efficiency of phytoremediation. The addition of organic acids from root exudates could alleviate the stress. In this study, three organic acids (citric acid, succinic acid, glutaric acid) were added to investigate the effects of organic acids on the stress response of Scirpus triqueter L. at two pyrene-lead concentrations. The activities of reactive oxygen species, malondialdehyde, plasma membrane H+-ATPase, and vacuolar H+-ATPase and PPase activity, as well as the activities of antioxidant enzymes (SOD, POD, and CAT) in Scirpus triqueter L. were determined. The addition of organic acids could effectively reduce the activities of reactive oxygen species, malondialdehyde, plasma membrane H+-ATPase, and vacuolar H+-ATPase and PPase activities. Under higher pollution, the damage of plant plasma membrane is more serious, but the addition of citric acid can alleviate this situation and even more effective than the relief under low pollution. The effect of citric acid was more significant than that of succinic acid and glutaric acid. These results demonstrated that organic acids could attenuate the stress of pyrene and lead to Scirpus triqueter L.