RESUMO
Objective: To retrospectively study the effects of budesonide inhalation combined with conventional symptomatic treatment on serum inflammatory factor expression levels and pulmonary function in patients with cough variant asthma (CVA) and to evaluate treatment efficacy. Methods: This retrospective cohort study included 200 patients diagnosed with CVA at the Second Hospital of Jiaxing between January 2022 and June 2023 and given conventional symptomatic treatment plus budesonide inhalation were included in this study. Patients were divided into a no remission group, a partial remission group and a complete remission group based on treatment effect. The expression levels of serum inflammatory factors, cough symptom scores, and small airway function indicators in the three groups of patients at different time points were compared. Results: In the three groups of CVA patients, after receiving budesonide inhalation combined with conventional symptomatic treatment, the expression levels of serum IL-5, IL-6, IL-8, TNF-α, TGF-ß1, and IgE and number of eosinophils significantly decreased (P <0.05). There were statistically significant differences in the IL-6 and TGF-ß1 levels among the three groups of CVA patients at T1, T2 and T3. There were statistically significant differences in IgE levels, number of eosinophils, cough symptom scores, and small airway function indicators between T2 and T3 (P<0.05). The receiver operating characteristic (ROC) curve prediction analysis revealed significant differences in the expression of IL-6 and TGF-ß1 at T1, T2, and T3. Conclusion: Budesonide inhalation combined with conventional symptomatic treatment can significantly reduce the levels of serum inflammatory factors in patients with CVA to reduce inflammation and the allergic response, thereby reducing the cough symptom score, improving pulmonary function, and improving therapeutic efficacy. In addition, IL-6 and TGF-ß1 can be used as early predictors of budesonide inhalation efficacy.
RESUMO
OBJECTIVE: To investigate the possible correlation between the microsatellite DNA instability (MSI), loss of heterozygosity (LOH ) on the long arm of chromosome 6 (6q) and the clinicopathologic features in Chinese patients with gastric cancer. METHODS: PCR-SSLP-Silver Stain method was used to detect four loci MSI and LOH in gastric tumor and paired normal control tissues of twenty and seven cases. RESULTS: In our study, the positive of MSI at one or more loci were observed in 16 out of 27 informative individuals (59.2%). 7 cases of 11 informative individuals without MSI were detected to happen the LOH at one or more loci (63.6%). And both MSI and LOH were frequently resulting at loci D6S434 (6q16.3-q21) and D6S404 (6q16.3-q23.2). MSI and LOH occurred in both well-differentiated and poor- differentiated gastric cancers, with a slight tendency of high grade MSI (MSI-H) existing chiefly in well-differentiated gastric cancer. CONCLUSION: The existence of MSI and/or LOH on 6q in 80% gastric carcinoma suggests that the genetic instability of 6q plays an important role in Chinese gastric cancer. The frequency of MSI and LOH has no significant relationship to clinicopathologic features such as age, gender, histological grade of differentiation and stages of tumor. The critical region of allelic deletion on 6q in Chinese gastric cancer is similar to those in other countries, and further confirms the presence of a putative tumor suppressor gene in this region.
