RESUMO
An octadecylsilyl porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. Five antipyretic drugs (aspirin, caffeine, guaiacol glycerol ether, 3-hydroxy-p-butyrophenetidine, and phenacetin) were separated in 2 min with a mobile phase of 20% acetonitrile at a flow-rate of 3.0 ml/min. A pharmaceutical preparation, containing aspirin, phenacetin, caffeine and chlorpheniramine maleate was analysed in 2 min with a mobile phase of acetonitrile-water-acetic acid (20:79:1). The packing seems promising for the rapid analysis of pharmaceuticals and biomedical compounds.
Assuntos
Anti-Inflamatórios não Esteroides/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Combinação de Medicamentos , Vidro , Tamanho da PartículaRESUMO
Using semimicro columns (150 X 1 mm I.D.) of octadecyl silica and styrene-divinylbenzene porous polymer, antipyretic drugs were analyzed by means of high-performance liquid chromatography. The antipyretics studied were sulpyrin, caffeine, guaiacol glycerol ether, acetaminophen, 3-hydroxy-p-butyrophenetidine, methyl p-hydroxybenzoate, phenacetin, mefenamic acid, aspirin, salicylamide, salicylic acid, o-ethoxybenzamide, theobromine, theophylline and their preparations. The semimicro columns are economical in solvents in chromatographic analysis.
