RESUMO
The interaction of acridine orange with blood albumins and tissue cells from different organs of white mouse has been studied by the spectral luminescence method. It was shown that acridine orange, by penetrating cells or orangelles, is able to intercalate between base pairs in the DNA molecule. It was found that the application of acridine orange as a fluorescent probe can influence the metabolic activity of organs.
Assuntos
Laranja de Acridina/farmacologia , Corantes Fluorescentes/farmacologia , Substâncias Intercalantes/farmacologia , Albumina Sérica/química , Laranja de Acridina/análise , Animais , DNA/química , DNA/metabolismo , Corantes Fluorescentes/análise , Substâncias Intercalantes/análise , Medições Luminescentes , Camundongos , Albumina Sérica/metabolismoRESUMO
The functional differences of blood serum albumin of some animal species have been studied using the spectral luminescent method by comparing the binding constants K and the concentration of binding sites N of rhodamine B and rhodanmine S to albumin. It was shown that K and N depend on both the concentration of blood serum albumin and animal species and the dye used.
