Effects of preservation of rumen inoculum on volatile fatty acids production and the community dynamics during batch fermentation of fruit pomace.

Rumen fluid (RF) as inocula is useful for evaluating biomass digestibility and has potential for producing volatile fatty acids (VFA) via the carboxylate platform. However, RF is not readily available, necessitating evaluation of potential preservation methods. Glycerol (50% v/v) and DMSO (5% v/v) were used to preserve rumen inocula for 3 months at -80 °C. Effects of cryo-preservation on digestibility, VFA production and community composition with ß-diversity distance metrics were compared to fresh RF using apple, citrus and grape pomace as substrates. For all substrates, DMSO cryo-preserved rumen digestibility parameters, VFA yield and product distribution were more significantly comparable to fresh RF (P > 0.05) than was glycerol cryo-preserved RF. Similarly, ß-diversity coefficient (unweighted unifrac) between DMSO cryo-preserved RF and fresh RF was 0.250 while the coefficient was 0.359 for the glycerol cryo-preserved RF compared to fresh RF. This showed that a DMSO cryo-preserved RF is less affected by preservation effects and is a more promising alternative to fresh RF.

Valorisation of the invasive species, Prosopis juliflora, using the carboxylate platform to produce volatile fatty acids.

Biomass derived from low-value, high-volume invasive plant species is an attractive, alternative feedstock to produce biofuels and biochemicals. This study aimed to use the carboxylate platform to valorize the invasive leguminous shrub, Prosopis juliflora (Mesquite), by utilizing in vitro rumen fermentations without chemical pretreatment to produce volatile fatty acids. The three fractions of the mesquite: leaves (ProL), stems (ProS) and branches (ProB) were compared regarding chemical composition, neutral detergent fiber (NDF) digestibility at 7 time points and VFA production after 72 h with sugarcane bagasse (SCB) as a reference. NDF digestibility was significantly (P < 0.05) higher in ProL (35.8%) than ProS (30.4%) and ProB (20.9%) compared to SCB (21.9%). VFA concentrations from 20 g biomass L-1 showed significant differences with 8.07, 6.71 and 6.51 g L-1 for ProL, ProS and ProB respectively, while SCB yielded 4.02 g L-1. These concentrations were comparable with other platforms that employ chemically pretreated biomass for VFA production.