RESUMO
Leishmania parasites isolated in the Islamic of Iran were studied by a random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). Of 82 isolates, 80 were from cutaneous lesions, 1 from a human throat lesion and 1 from a dog. Of these, 42 isolates were L. tropica, 36 were L. major and 2 were L. infantum. There were 2 unidentified isolates (from the throat lesion and a cutaneous lesion) and these demonstrated 52% and 48% similarity with L. tropica and L. infantum. Both L. tropica and L. major were isolated from four provinces indicating a recent change in the epidemiology of cutaneous leishmaniasis. L. tropica was isolated from three provinces; L. major from one province. L. infantum was isolated from a human cutaneous lesion and from a dog in Bushehr province.
Assuntos
Leishmania infantum , Leishmania major , Leishmania tropica , Leishmaniose/epidemiologia , Leishmaniose/parasitologia , Animais , Antiprotozoários/uso terapêutico , DNA de Protozoário/análise , DNA de Protozoário/genética , Reservatórios de Doenças/estatística & dados numéricos , Reservatórios de Doenças/veterinária , Doenças do Cão/epidemiologia , Doenças do Cão/parasitologia , Cães , Eletroforese em Gel de Ágar , Doenças Endêmicas/estatística & dados numéricos , Variação Genética/genética , Humanos , Irã (Geográfico)/epidemiologia , Leishmania infantum/genética , Leishmania infantum/isolamento & purificação , Leishmania major/genética , Leishmania major/isolamento & purificação , Leishmania tropica/genética , Leishmania tropica/isolamento & purificação , Leishmaniose/prevenção & controle , Leishmaniose/veterinária , Seleção de Pacientes , Vigilância da População , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos , Características de Residência/estatística & dados numéricosRESUMO
Leishmania parasites isolated in the Islamic Republic of Iran were studied by a random amplified polymorphic DNA polymerase chain reaction [RAPD-PCR]. Of 82 isolates, 80 were from cutaneous lesions, 1 from a human throat lesion and 1 from a dog. Of these, 42 isolates were L. tropica, 36 were L. major and 2 were L. infantum. There were 2 unidentified isolates [from the throat lesion and a cutaneous lesion] and these demonstrated 52% and 48% similarity with L. tropica and L. infantum. Both L. tropica and L. major were isolated from four provinces indicating a recent change in the epidemiology of cutaneous leishmaniasis. L. tropica was isolated from three provinces; L. major from one province. L. infantum was isolated from a human cutaneous lesion and from a dog in Bushehr province
