Hemiodus bimaculatus, a new species of Hemiodontidae from the Rio Tapajós drainage, Brazil (Ostariophysi: Characiformes).

Hemiodus bimaculatus sp. nov., is described from tributaries of the Rio Juruena and Rio Teles Pires in the upper Rio Tapajós basin. The new species is diagnosed from most congeners, except Hemiodus jatuarana, by having a conspicuous circular or horizontally elongate dark blotch on the caudal peduncle (v. inconspicuous in H. iratapuru and absent in the other species). The new species differs from H. jatuarana by having a round midlateral spot on the flank (v. absent in H. jatuarana), 98-121 perforated scales in the lateral line (v. 66-72 in H. jatuarana), 23-28 scale series above and 14-19 below lateral line (v. 12-13 above and 6-7 below in H. jatuarana). Hemiodus bimaculatus is hypothesised to be related to species of the H. microlepis group, from which it also differs by having 11-25 epibranchial (v. 26-34 in H. argenteus, 29-39 in H. microlepis, 21-42 in H. orthonops and 27-35 in H. parnaguae) and 18-31 ceratobranchial (v. 38-50 in H. argenteus, 43-58 in H. microlepis, 32-52 in H. orthonops and 34-48 in H. parnaguae) gill rakers in the first arch.

Tetrapod limb and sarcopterygian fin regeneration share a core genetic programme.

Salamanders are the only living tetrapods capable of fully regenerating limbs. The discovery of salamander lineage-specific genes (LSGs) expressed during limb regeneration suggests that this capacity is a salamander novelty. Conversely, recent paleontological evidence supports a deeper evolutionary origin, before the occurrence of salamanders in the fossil record. Here we show that lungfishes, the sister group of tetrapods, regenerate their fins through morphological steps equivalent to those seen in salamanders. Lungfish de novo transcriptome assembly and differential gene expression analysis reveal notable parallels between lungfish and salamander appendage regeneration, including strong downregulation of muscle proteins and upregulation of oncogenes, developmental genes and lungfish LSGs. MARCKS-like protein (MLP), recently discovered as a regeneration-initiating molecule in salamander, is likewise upregulated during early stages of lungfish fin regeneration. Taken together, our results lend strong support for the hypothesis that tetrapods inherited a bona fide limb regeneration programme concomitant with the fin-to-limb transition.