RESUMO
Un sistema de diagnóstico para la detección temprana del virus Dengue 1 fue llevado a cabo exitosamente usando la reacción en cadena por polimerasa de trnscriptasa reversa (RT-PCR), a través de la amplificación de una porción genómica del gen NS1. Los resultados obtenidos, a partir de muestras clínicas, corroboraron los datos de anteriores trabajos de RT-PCR dirigidos hacia la región estructural del virión. POsteriormente el ADNc del virus Dengue, correpondiente a una de las muestras serológicas, fue clonado y secuenciado. La comparación por ánalisis de secuencia nucleotídica con otras cepas referenciales determino que la cepa viral correspondía al serotipo 1
Assuntos
Reação em Cadeia da Polimerase Via Transcriptase Reversa , DengueRESUMO
The diagnostic potential of recombinant leishmanial antigens for Latin American tegumentary leishmaniasis (LATL) was examined. Two Leishmania (Viannia) peruviana recombinant proteins, T26-U2 and T26-U4, were assessed by their reactivity to detect specific anti-leishmanial antibodies. Seventy-eight individual sera from persons with LATL, 39 from those with other diseases, and 10 negative control sera were tested by Western blotting and enzyme-linked immunosorbent assay. The sensitivity of the test using T26-U2 plus T26-U4 was similar to that obtained with whole parasite extract (92%). However, the specificity obtained using both recombinant antigens (87%) was higher than that of the whole parasite extract (65%). All tests using recombinant proteins (T26-U2, T26-U2 plus T26-U4 or T26-U4) had a higher positive predictive value (89%, 92% and 98%, respectively) than the value obtained using total parasites (81%). Eleven Colombian sera were also tested, and the results indicated that T26-U2 plus T26-U4 could be used successfully in Peru and in other Latin American countries.
