An acid-tolerant ammonia-oxidizing γ-proteobacterium from soil.

Nitrification, the microbial oxidation of ammonia to nitrate via nitrite, occurs in a wide range of acidic soils. However, the ammonia-oxidizing bacteria (AOB) that have been isolated from soil to date are acid-sensitive. Here we report the isolation and characterization of an acid-adapted AOB from an acidic agricultural soil. The isolated AOB, strain TAO100, is classified within the Gammaproteobacteria based on phylogenetic characteristics. TAO100 can grow in the pH range of 5-7.5 and survive in highly acidic conditions until pH 2 by forming cell aggregates. Whereas all known gammaproteobacterial AOB (γ-AOB) species, which have been isolated from marine and saline aquatic environments, are halophiles, TAO100 is not phenotypically halophilic. Thus, TAO100 represents the first soil-originated and non-halophilic γ-AOB. The TAO100 genome is considerably smaller than those of other γ-AOB and lacks several genes associated with salt tolerance which are unnecessary for survival in soil. The ammonia monooxygenase subunit A gene of TAO100 and its transcript are higher in abundance than those of ammonia-oxidizing archaea and betaproteobacterial AOB in the strongly acidic soil. These results indicate that TAO100 plays an important role in the nitrification of acidic soils. Based on these results, we propose TAO100 as a novel species of a new genus, Candidatus Nitrosoglobus terrae.

Time series changes in radiocaesium distribution in tea plants (Camellia sinensis (L.)) after the Fukushima Dai-ichi Nuclear Power Plant accident.

Radiocaesium ((134)Cs and (137)Cs) release following the accident at the Fukushima Dai-ichi Nuclear Power Plant, belonging to the Tokyo Electric Power Company caused severe contamination of new tea plant (Camellia sinensis (L.)) shoots by radiocaesium in many prefectures in eastern Japan. Because tea plants are perennial crops, there is the fear that the contamination might last for a long time. The objectives of this study were to reveal time series changes in the distribution of radiocaesium in tea plants after radioactive fallout and to evaluate the effect of pruning on reduction of radiocaesium concentrations in new shoots growing next year. The experimental tea field was located in Shizuoka, Japan, approximately 400 km away from the Fukushima Dai-ichi Nuclear Power Plant in a southwest direction. Time series changes in radiocaesium concentrations in unrefined tea, a tea product primarily produced for making Japanese green tea, from May 2011 to June 2013 and distribution of radiocaesium in tea plants from May 2011 to May 2012 were monitored. The radiocaesium concentrations in unrefined tea exponentially decreased; the effective half-lives for (134)Cs and (137)Cs were 0.30 and 0.36 y during the first 2 y after the accident, respectively. With time, the highest concentrations of (137)Cs moved from the upper to the lower parts of plants. Medium pruning 2-3 months after the accident reduced the concentration of (137)Cs in new shoots harvested in the first crop season of the following year by 56% compared with unpruned tea plants; thus, pruning is an effective measure for reducing radiocaesium concentration in tea.