RESUMO
The decocted extract of Uncariae Ramulus et Uncus, for which prolonged boiling is presumed to reduce its activities, was extracted with n-hexane-chloroform (5: 1) and methanol consecutively. The patterns of each organic solvent extract were analysed with thinlayer chromatography (TLC) by several solvent systems using the Dual-wavelength TLC Scanner. No clear difference in the patterns was evident in any of the 10-120 min decocted extracts.<BR>The comparison of patterns of the chromatograms however revealed some new informations.
RESUMO
The loss of essential oil during infusing Anchu-san was examined. After six minutes boiling, 22.7% of oil passed into the decoction and only 7.8% of oil remained when infused till half volume.<BR>The contents of essential oil in diverse preparations of Anchu-san on the market were also compared to find that all of the medicines contained far less oil than decoction.<BR>Then, simultaneous analysis of essential oils in Cinnamomi Cortex, Foeniculi Fructus and Amomi Semen, which constitute Anchu-san, by using high-performance liquid chromatography was investigated. The constituents of oils, cinnamaldehyde, D-camphor, borneol, linalool, <BR>anethole and bornyl acetate, were separated by using μ-Bondapak C13 as column and water: methanol (40: 60) as eluate. From the patterns of chromatograms of the decoction and several preparations, some new informations were provided.
