Assuntos
Proteínas de Bactérias/metabolismo , Escherichia coli/metabolismo , Vitamina B 12/metabolismo , Animais , Soluções Tampão , Bovinos , Cromatografia em Gel , Cromatografia em Papel , Meios de Cultura , Diálise , Escherichia coli/efeitos dos fármacos , Glucose/farmacologia , Concentração de Íons de Hidrogênio , Peso Molecular , Concentração Osmolar , Ligação Proteica , Soroalbumina Bovina/metabolismo , Tensoativos/farmacologia , Temperatura , Fatores de Tempo , Trítio , VibraçãoRESUMO
A pink, adenine-requiring yeast utilized adenine, hypoxanthine, or S-adenosylmethionine (SAM), in quantities up to 3 mumoles per 100 ml of medium, as equivalent sources of purine for cell growth, but not methylthioadenosine or S-adenosylhomocysteine. Utilization of SAM for growth was inhibited by the presence of l-methionine in quantities greater than 0.6 mumole per 100 ml of medium. However, 6 mumoles of l-methionine had no effect on growth when adenine or hypoxanthine was the source of purine. These sources also reversed the inhibitory effects of 6 mumoles of the amino acid on the utilization of SAM. The presence of 400 mumoles of the amino acid resulted in some inhibition of growth when the organisms were grown with adenine, hypoxanthine, or adenine plus SAM but had no effect on the total uptake of adenine-8-(14)C. Studies on the uptake of radioactivity from a mixture of SAM-adenine-8-(14)C and (3)H-labeled SAM-methyl indicated that these components were taken into the cells at different rates which were altered by the presence of l-methionine. The fixation of (35)S from (35)S-labeled adenosylmethionine into the cells was inhibited by the presence of the amino acid. The cells synthesized and accumulated SAM in the presence of 400 mumoles of l-methionine plus adenine even when exogenous SAM was supplied. Approximately 47% of radioactivity fixed from exogenous SAM-adenine-8-(14)C and 12% from (3)H-labeled SAM-methyl were found in reisolated SAM.
