The effects of peppermint (Mentha piperita L.) and chicory (Cichorium intybus L.) in comparison with a prebiotic on productive performance, blood constituents, immunity and intestinal microflora in broiler chickens.

A total of 320 one-day-old broiler chickens were used in a 42-day feeding trial to evaluate the effects of peppermint (Mentha piperita L.) and chicory (Cichorium intybus L.) in comparison with a prebiotic on-growth performance, blood constitutes, immunity and intestinal microflora. The dietary treatments were as follows: basal diet (control); control + prebiotic (Fermacto™); control + 0.1% peppermint; control + 0.1% chicory, respectively. A significant (p < 0.05) body weight gain and feed intake was found at 21 and 42 days of growth period in broilers fed diet supplemented with 0.1% chicory compared with other groups. Feeding of prebiotic or chicory led to higher (p < 0.05) feed intake. Chickens fed control diet had higher (p < 0.05) abdominal fat compared with the other groups. Serum blood constituents indicated that broilers fed prebiotic or supplemented with peppermint or chicory had reduced (p < 0.05) levels of cholesterol, triglycerides and low-density lipoprotein than control group. Immunity-related parameters showed that chicken fed chicory had lower (p < 0.05) heterophil-to-lymphocyte ratio compared with the other groups. Intestinal microflora revealed that chickens fed prebiotic or herbals had higher count of Lactobacillus and lower E. coli than control. Thus, it can be concluded that broiler dietary supplementation with prebiotic or chicory can improve performance supporting positively health status.

The Effects of Turnip (Brassica rapa) Extract on the Growth Performance and Health of Broilers.

There are concerns about inclusion of antibiotics in the feed of broiler chickens, because of the development of antibiotic resistance, leading to a search for alternative feed additives. Turnip extract is known to have antibacterial properties but has not been tested in the diet of broiler chickens. We allocated 200 broiler chicks to receive one of four levels of turnip extract in their water, 0, 150, 300 or 450 ppm, or a standard antibiotic, Virginiamycin, over a 42-day growing period. Although initially there were detrimental effects of providing 450 ppm, overall the 150 ppm level of supplementation increased weight gain, compared with birds given Virginiamycin, and decreased gizzard weight. Birds given 150 ppm or Virginiamycin had increased low-density lipoproteins (LDLs) and reduced very low-density lipoproteins (VLDLs) in their blood serum and reduced antibody responses to sheep red blood cells, compared to birds in the 450 ppm treatment. Birds given turnip extract at 450 ppm had fewer Gram-negative lactose and coliform bacteria than those provided with no turnip extract, and those provided with 150-300 ppm had the same as those provided with Virginiamycin. Turnip extract could potentially replace antibiotics included in the feed of broiler chickens for growth promotion and the control of bacterial infection of the gastrointestinal tract.

Effects of Dietary Chicory (Chicorium intybus L.) and Probiotic Blend as Natural Feed Additives on Performance Traits, Blood Biochemistry, and Gut Microbiota of Broiler Chickens.

The experiment was designed to determine the effect of different levels of chicory (Chicorium intybus L.) powder and a probiotic blend (PrimaLac®) on productive performance, blood biochemical parameters, and ileal microbiota in broiler chickens. A total of 225 one-day-old broilers (Ross 308) were used in a completely randomized design with five experimental diets as follows: 1-basal-diet without supplements (control-group); 2-basal-diet including probiotic blend; 3- basal-diet including 0.10% chicory; 4-basal-diet including 0.15% chicory; 5-basal-diet including 0.20% chicory. At 42 days of age, representative birds per replicate were randomly selected for blood samples and carcass measurements. Results showed that the body weight gain of broilers fed the probiotic blend or 0.10% chicory was significantly (P < 0.05) higher than those fed on the other treatments. The abdominal fat pad was significantly (P < 0.05) lower in birds fed diets including chicory compared with control or probiotic. Blood triglycerides and LDL levels were reduced (P < 0.05) and HDL increased (P < 0.05) when fed probiotic or chicory whereas no significant effect on the other serum parameters was found. Broiler ileal microflora from the control group had significantly (P < 0.05) higher count of E. coli and lower Lactobacillus than those from the other groups. From findings, it is possible to conclude that dietary chicory powder supported positively growth performance and improved gut microbiota in broiler chickens. However, more research is needed on this subject to better understand the mode of action of feed additives used.

Dietary supplementation of Aspergillus oryzae meal and its effect on performance, carcass characteristics, blood variables, and immunity of broiler chickens.

This study investigated the effect of different levels and consumption periods of Aspergillus oryzae meal on performance, carcass characteristics, blood variables, and immunity of broiler chickens. A total of 270 (male and female) Ross 308 chicks were randomly assigned to 9 treatment groups. Two levels (2 g/kg diet and 4 g/kg diet as-fed) of Aspergillus oryzae meal (AO) and 4 consumption periods of AO (starter, grower, finisher, and entire period) in a 2 × 4 factorial arrangement were used. Compared with control, AO used during the entire rearing period increased weight gain, reduced relative weight of abdominal fat, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) serum levels, and increased antibody titers against influenza and Newcastle disease vaccination and sheep red blood cells injection. Few differences in the variables considered were found if AO was added to broiler diets only during specific consumption periods, and between the two supplementation levels of AO. In conclusion, the addition of AO to the broiler diet can have beneficial effects in terms of performance, carcass composition, and health, but these positive effects were mainly reached adding AO for the entire rearing period.

Effects of Hogweed (Heracleum persicum) Powder, Flavophospholipol, and Probiotics as Feed Supplements on the Performance, Carcass and Blood Characteristics, Intestinal Microflora, and Immune Response in Broilers.

The effect of different levels of hogweed powder (HP; Heracleum persicum), flavophospholipol (antibiotic), and probiotics in diet on the performance, carcass quality, blood biochemical parameters, immunity, and intestinal flora of broiler chickens was investigated. In total, 270-day-old male broilers were randomly assigned to six treatment groups as follows: control basal-diet and diet supplemented with flavophospholipol, probiotics, or 0.25, 0.5, and 0.75% HP. Birds in each group were divided into three subgroups with 15 chicks each. Results indicated that the treatment groups did not vary with respect to feed intake (FI), whereas those supplemented with the antibiotic or 0.5% HP showed significantly higher body weight gain (BWG) and improved feed conversion ratio (FCR). Carcass characteristics did not vary among treatments, with the exception of abdominal fat percentage, which was the lowest in broilers fed 0.5% and 0.75% HP. Supplementation of 0.5% and 0.75% HP decreased plasma cholesterol and triglyceride levels. Furthermore, dietary HP significantly reduced serum low density lipoprotein (LDL) levels compared to that in the other groups. Antibody titers against Newcastle disease vaccine were not markedly affected by the treatments, whereas titers against avian influenza vaccine were significantly higher in probiotic- and 0.75% HP-supplemented groups. Antibody production against sheep red blood cells (SRBC) and IgM and IgG levels were not significantly different among groups. The ileum Lactobacillus counts in broilers fed 0.5% or 0.75% HP were significantly higher than those in the other treatment groups, whereas Escherichia coli counts in all treatments were significantly lower than that in the control. Therefore, our observations indicated that HP positively affected the gut microbiota and enhanced feed digestion. In conclusion, supplementation of 0.50-0.75% HP in broiler diet during the entire rearing period improved BWG and decreased abdominal fat deposition.

Effect of Dietary Ginger (Zingiber officinale Roscoe) and Multi-Strain Probiotic on Growth and Carcass Traits, Blood Biochemistry, Immune Responses and Intestinal Microflora in Broiler Chickens.

A total of 225 day-old male broiler chicks (Ross-308) were randomly allocated to five treatment groups, with three replicates in a completely randomized design for 42 days. Birds were fed a basal-diet supplemented with: no additive (control-diet), multi-strain probiotic (Lactobacillus acidophilus, Lactobacillus casei, Enterococcus faecium and Bifidobacterium thermophilum), or 0.15, 0.20 and 0.25% ginger (Z. officinale) powder, respectively. The results show no significant differences among treatments for growth traits and carcass characteristics, whereas using probiotics and ginger at all levels resulted in a significant decrease of gizzard weight and abdominal fat compared to the control group. Dietary treatments did not affect blood biochemistry and antibody production against sheep red blood cells (SRBC), IgG and IgM; however, antibody titre was higher in birds fed 0.25% ginger than other diets after 7 days post injection. The Lactobacillus counts in ileal content of birds fed 0.20 and 0.25% ginger were higher compared to the other treatments. In conclusion, dietary supplementation with either ginger or probiotics showed a significant influence on birds' immune response, probably because ginger had strong antioxidant activity and the probiotics stimulated the production of natural antibodies.