Long-Term Vaccine Delivery and Immunological Responses Using Biodegradable Polymer-Based Carriers.

Biodegradable polymers are largely employed in the biomedical field, ranging from tissue regeneration to drug/vaccine delivery. The biodegradable polymers are highly biocompatible and possess negligible toxicity. In addition, biomaterial-based vaccines possess adjuvant properties, thereby enhancing immune responses. This Review introduces the use of different biodegradable polymers and their degradation mechanism. Different kinds of vaccines, as well as the interaction between the carriers with the immune system, then are highlighted. Natural and synthetic biodegradable micro-/nanoplatforms, hydrogels, and scaffolds for local or targeted and controlled vaccine release are subsequently discussed.

Preparation of amylose-poly(methyl methacrylate) inclusion complex as a smart nanocarrier with switchable surface hydrophilicity.

Amylose, as a linear biopolymer, tends to form helical inclusion complexes with suitable guest species. This is of great importance for a variety of applications, especially in the pharmaceutical and food industry. In this study, we propose an approach for the preparation of a novel inclusion complex with switchable surface hydrophilicity. For this purpose, amylose was first conjugated to ethylene diamine hydrophilic residues. Then, the short chains of the hydrophobic poly(methyl methacrylate, PMMA) were grafted onto the cavity of amylose through atom transfer radical polymerization (ATRP). According to CD spectroscopy results, the amylose-PMMA inclusion complexes displayed solvent-directed helical chirality inversion using either DMSO or water as a solvent. Fluorescence imaging, AFM and DLS techniques revealed the solvent-dependent surface hydrophilicity of the amylose-PMMA inclusion complex. Interestingly, its morphological studies displayed a central cavity, which makes it suitable for carrying cargoes in drug delivery applications. Obtaining the amylose-polymer inclusion complexes with tailorable hydrophilicity of both the exterior surface and the interior cavity can be of paramount importance for a wide variety of bio-applications.

Diaminated Starch: A Competitor of Chitosan with Highly Mucoadhesive Properties due to Increased Local Cationic Charge Density.

The purpose of this study was to synthesize diaminated starch as a novel mucoadhesive polymer. Starch was tosylated and then reacted with ethylenediamine. The degree of amination was determined by 2,4,6-trinitrobenzene sulfonic acid assay. Properties of diaminated starch including solubility, cytotoxicity, swelling behavior, and mucoadhesion were compared to chitosan. Diaminated starch displayed 2083 ± 121.6 µmol of diamine substructures/g of polymer. At pH 6, diaminated starch exhibited a ζ potential of 6 mV, whereas it was close to zero in the case of unmodified starch. In addition, diaminated starch displayed water solubility over the entire pH range and minor cytotoxicity. The novel polymer showed pronounced swelling behavior in water increasing its initial weight 18- and 6-fold at pH 5 and 6, respectively. Moreover, diaminated starch exhibited 92-fold higher-mucoadhesivity properties than those of chitosan. According to these results, diaminated starch might be a promising novel excipient for the design of mucoadhesive formulations.