Comparison of diurnal biting activity, life table, and demographic attributes of Aedes albopictus (Asian tiger mosquito) from different urbanized settings in West Java, Indonesia.

The Asian tiger mosquito, Aedes albopictus has well-adaptive behavior to environmental changes, including human urbanization, and has an essential role as the main vector of important pathogenic arboviruses. This study aims to analyze the biology and life table of the Ae. albopictus populations collected from urban and peri-urban areas of Bogor, West Java, Indonesia under laboratory conditions. Mosquito eggs collection was carried out in urban and peri-urban areas using ovitraps. The observation of the life table experiment that followed the development of Ae. albopictus started from the emergence of the first individual to the last surviving individual. Several biological parameters comparing Ae. albopictus from two collection sites based on life table analysis were shown to be significantly different. Biting activity of all mosquitoes from urban and peri-urban areas showed a clear bimodal activity with morning peak at 09:00-10.00 and evening peak at 16:00-17:00. Ae. albopictus from the urban area have higher fecundity, considerably longer lifespan, more gonotrophic cycles, and a higher net reproduction rate (R0) than Ae. albopictus from the peri-urban area. These findings will provide valuable information about the well-adapted Ae. albopictus in urban areas and assist in providing basic reproductive data to improve vector control and current surveillance strategies especially in the study area.

Diversity of mosquito species and potential arbovirus transmission in long-tailed macaque (Macaca fascicularis) breeding facilities.

Background and Aim: Mosquito-borne viral infections are diseases that reduce human and animal health levels. Their transmission involves wildlife animals as reservoirs and amplifying hosts, including long-tailed macaques (Macaca fascicularis), and potentially transmits to humans and vice versa. This study aimed to determine the species diversity, richness, and biting activity of mosquitoes in a long-tailed macaque breeding area facility and discover the presence of Flavivirus and Alphavirus as the two main arboviruses reported to infect macaques. Materials and Methods: Human landing catch, light trap, and sweep net methods were used for mosquito collection around long-tailed macaques cages at parallel times for 12 h (18:00-06:00) for 12 nights. Mosquito species were identified to the species level based on the morphological identification key for Indonesian mosquitoes. Mosquito diversity was analyzed by several diversity indices. Mosquitoes caught using the human landing catch method were pooled based on mosquito species for viral ribonucleic acid extraction. Reverse transcription-polymerase chain reaction (RT-PCR) detected the non-structural protein 5 of the Flavivirus region and the non-structural protein 4 of the Alphavirus region. This study used the man-hour density and man-biting rate formulas for mosquito density. Results: Ten mosquito species were collected, namely, Aedes albopictus, Anopheles aconitus, Anopheles minimus, Anopheles vagus, Armigeres foliatus, Armigeres subalbatus, Culex gelidus, Culex hutchinsoni, Culex tritaeniorhynchus, and Culex quinquefasciatus. The number of mosquitoes caught using the light trap method had the highest abundance. In contrast, the number of mosquito species caught using the sweep net method had lower diversity than the other two methods. Seven mosquito species were obtained using the human landing catch method. The mosquito species with the highest density was Cx. quinquefasciatus within the observed densest period from 20:00 to 21:00. Negative results were obtained from RT-PCR testing on five species detected using universal Flavivirus and Alphavirus primers. Conclusion: The occurrence of mosquitoes in long-tailed macaque breeding facilities can be a source of transmission of zoonotic vector-borne diseases between animals and humans and vice versa.