RESUMO
AIM: To identify risk factors and predictors of pregnancy loss and to compare the efficacy of Arabin's pessary with cervical cerclage in women at a high risk of pregnancy loss. MATERIALS AND METHODS: This was a two-center retrospective case-control study that included 240 women at a high risk of preterm delivery. Group I (n = 161) included women who underwent insertion of the Arabin's pessary between 14 and 24 weeks of pregnancy. Group II (n = 79) included women who had undergone circular cervical cerclage during the current pregnancy. All women included in the study received micronized vaginal progesterone at the dose of 200 mg/day until and including 34 weeks of gestation. RESULTS: Threatened pregnancy loss defined as spotting or vaginal bleeding in the first trimester was diagnosed in 29.8% (48/161) of patients in Group I versus 37.9% in Group II (p = .448). Postpartum bleeding occurred in 8.1% (13/161) in women in Group I versus 22.8% in Group II (χ2 = 6.500; p = .011). Our study showed that cervical cerclage was most suitable for patients with history of obstetric complications, cervical length <15 mm, and large isthmic uterine fibroids. The use of the Arabin's pessary reduced the rate of preterm births by 1.7-fold. A cluster analysis demonstrated that predictors of preterm birth in women with a high risk of pregnancy loss included: threatened pregnancy loss associated with chorionic/placental abruption, cervical incompetence, uterine fibroid growth to a large size, history of multiple spontaneous pregnancy losses, cervical tears during past labor, and gestational diabetes diagnosed for the first time during the current pregnancy. CONCLUSIONS: Women with a high risk of pregnancy loss treated with Arabin's pessary or cerclage plus vaginal progesterone had a term delivery rate of 70.4% (169/240). The combined strategy of pregnancy management allowed to markedly reduce the number of preterm births.
Assuntos
Cerclagem Cervical , Nascimento Prematuro , Incompetência do Colo do Útero , Estudos de Casos e Controles , Feminino , Humanos , Recém-Nascido , Placenta , Gravidez , Gestantes , Nascimento Prematuro/epidemiologia , Nascimento Prematuro/etiologia , Nascimento Prematuro/prevenção & controle , Estudos Retrospectivos , Fatores de Risco , Incompetência do Colo do Útero/epidemiologia , Incompetência do Colo do Útero/cirurgiaRESUMO
The article covers forecasting work conditions in oncoming production of sulphenamides T and DC--vulcanization accelerators, specifies leading occupational hazards, justifies values of MAC for sulphenamide T and approximate safe levels of action for sulphenamide DC in air of workplace.
Assuntos
Indústrias , Doenças Profissionais/prevenção & controle , Exposição Ocupacional/prevenção & controle , Saúde Ocupacional , Sulfamerazina/efeitos adversos , Toxicologia/métodos , Humanos , Doenças Profissionais/etiologia , Exposição Ocupacional/efeitos adversos , Prognóstico , Federação RussaRESUMO
Ninety-six patients with chorioamnionitis in labor were treated. Clinical observations and laboratory findings demonstrated high efficiency of multiple-modality treatment including use of efferent methods: plasmapheresis, incubation of cell mass with antibiotics, and ultra-violet exposure of the blood.
Assuntos
Complicações Pós-Operatórias/terapia , Infecção Puerperal/terapia , Cesárea , Corioamnionite/terapia , Terapia Combinada , Feminino , Humanos , Complicações do Trabalho de Parto/terapia , Cuidado Pós-Natal/métodos , Cuidados Pós-Operatórios/métodos , Gravidez , Fatores de Risco , Fatores de TempoRESUMO
SUP2 (SUP35) is an omnipotent suppressor gene, coding for an EF-1 alpha-like protein factor, intimately involved in the control of translational accuracy in yeast Saccharomyces cerevisiae. In the present study a SUP2 gene analogue from yeast Pichia pinus was isolated by complementation of the temperature-sensitive sup2 mutation of S. cerevisiae. The nucleotide sequence of the SUP2 gene of P. pinus codes for a protein of 82.4 kDa, exceeding the Sup2 protein of S. cerevisiae by 6 kDa. Like the SUP2 gene product of S. cerevisiae, the Sup2 protein of P. pinus represents a fusion of a unique N-terminal part and a region homologous to EF-1 alpha. The comparison of amino acid sequences of the Sup2 proteins reveals high conservation (76%) of the C-terminal region and low conservation (36%) of the N-terminal part where, in addition, the homologous correspondence is ambiguous. Proteins related to the Sup2 of S. cerevisiae were found in P. pinus and some other yeast species by the immunoblotting technique. The relation between the evolutionary conservation of different regions of the Sup2 protein and their functional significance is discussed.
Assuntos
Proteínas Fúngicas/genética , Genes Supressores , Pichia/genética , Príons , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Códon/genética , DNA Fúngico/genética , Proteínas Fúngicas/análise , Immunoblotting , Dados de Sequência Molecular , Fatores de Terminação de Peptídeos , Plasmídeos , Sequências Repetitivas de Ácido Nucleico , Mapeamento por Restrição , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Transcrição Gênica , Transformação GenéticaRESUMO
SUP2(SUP35) is an omnipotent suppressor gene, coding for an EF-1 alpha-like protein factor, involved in the control of translational accuracy in yeast Saccharomyces cerevisiae. A SUP2 gene analogue from yeast Pichia pinus was isolated by complementation of temperature-sensitive sup2 mutation of S. cerevisiae. Nucleotide sequence of the SUP2 gene of P. pinus codes for a protein of 82.4 kDa exceeding the SUP2 protein of S. cerevisiae for 6 kDa. The SUP2 gene product of P. pinus is similar to the Sup2 protein of S. cerevisiae by its structure and includes a highly conservative (76%) C-terminal region homologus to EF-1 alpha and a lowly conservative N-terminal region. The relation between the evolutionary conservativity of different regions of the Sup2 protein and their functional significance is discussed.