RESUMO
Five continuous lymphoid suspension cell cultures were established by cultivation of lymphocytes from African green monkeys. The immunological typing revealed the mixed pattern of cultures consisting of B and T cell types. Two types of viruses: herpesvirus and retrovirus C-type were demonstrated by electron microscopy. Indirect immunofluorescence and molecular hybridization studies showed one of the viruses to be an EBV-related herpesvirus of green monkeys designated HVGM and the other to belong to H (S) TLV-1 group.
Assuntos
Chlorocebus aethiops/microbiologia , Herpesviridae/isolamento & purificação , Vírus Linfotrópico T Tipo 1 Humano/isolamento & purificação , Linfócitos/microbiologia , Animais , Anticorpos Monoclonais , Separação Celular , Células Cultivadas/microbiologia , Células Cultivadas/ultraestrutura , DNA/genética , DNA Viral/genética , Imunofluorescência , Herpesviridae/genética , Herpesviridae/ultraestrutura , Vírus Linfotrópico T Tipo 1 Humano/genética , Vírus Linfotrópico T Tipo 1 Humano/ultraestrutura , Linfócitos/ultraestrutura , Microscopia Eletrônica , Hibridização de Ácido Nucleico , Vírion/genética , Vírion/isolamento & purificação , Vírion/ultraestrutura , Cultura de VírusRESUMO
The aim of this work was to study genomes of B-lymphotropic oncogenic herpesviruses of Macaca arctoides (HVMA), Papio hamadryas (HVP) and Cercopithecus aethiops (HVGM). Blot-hybridization detected inter- and intraspecific genome heterogeneity of investigated viruses in restriction sites EcoR1, Pst1 and Hind111. Virus-specific sequences in two-cell lines obtained from HVMA-induced rabbit tumours can prove HVMA a role in malignant tumour induction in rabbits.
