[Local cerebral consumption of glucose in the mouse. Adaptation of the 14C deoxyglucose method to free-moving small mammals]. / Consommation cérébrale locale de glucose chez la souris. Adaptation de la méthode du [14C] désoxyglucose à un petit mammifère libre de se mouvoir.

Local cerebral glucose utilisation (LCGU) was measured with the [14C] deoxyglucose ([14C] DG) method in the awake, free moving Mouse. Values vary from 112 mumoles. 100 g-1. min.-1 in the superior colliculus, the most active structure, to 22 mumoles. 100 g-1. min.-1 in the internal capsule. Glucose consumption is high in fasciculus retroflexus, a result peculiar to the Mouse when compared to the other Mammals were studied so far with this method. The modifications in plasma sampling proposed in this paper should facilitate the use of the [14C] DG method in free moving animals as small as the Mouse, and allow for study of the effects of behavior on local cerebral energy consumption.

Application of the 2-deoxy-D-[14C]-glucose method to the mouse for measuring local cerebral glucose utilization.

The 2-deoxy-D-[14C]-glucose method for the quantitative determination of local cerebral glucose utilization has been applied to the mouse. The experiments were conducted in awake freely moving animals. Local cerebral glucose utilization was found to vary widely throughout the brain with the lowest values in white matter and the highest in gray matter. The most metabolically active structures were those involved in auditory function or in general motor activity. All the values were lower than those observed previously in the conscious rat. The use of free-moving animals may be of importance since it allows the study of physiological or pathological behavioral states.

A longitudinal study of bioelectric activity in the pre- and post-hatch chick.

A technique for embryonic implantation and the subsequent recording of electrocortical, neck muscle, and ocular activity continously from the 20th day of incubation through hatching and the first few days thereafter is demonstrated. The embryonic maturation of the EEG, with a characteristic muscle burst pattern heralding hatching was found, supporting previous reports obtained with acute preparations. The technique for injection into the chorioallantoic membrane (CAM) vessels or direct deposition onto the CAM is also described. The usefulness of the embryonic neurophysiological implantation coupled with the injection at specific stages of development is discusses as an approach to the understanding of the parameters of the maturation of the sleep-wakefulness cycle, neurochemistry, and behavior.

Effects of early post-natal alpha-methyl-dopa treatment on behavior in the rat.

250 mg/kg of L-alpha-methyl-Dopa (alpha-m-Dopa) or saline were administered to rats for three weeks after birth. Subsequent tests revealed an increased locomotor activity and greater rate of acquisition but no disturbance in shuttle-box conditioning. The whole brain content of norepinephrine, dopamine and serotonin was not affected.