Effect of ultrasound on the interleukin content in blood of rats with experimental inflammation. / VPLYV UL'TRAZVUKU NA VMIST INTERLEiKINIV U KROVI ShchURIV Z EKSPERYMENTAL'NYM ZAPALENNIaM.

UNLABELLED: The aim was to determine the effectiveness of anti-inflammatory action of ultrasound by the interleukin-6, interleukin-8 and tumor necrosis factor alpha content in the blood of rats with experimental inflammation. MATERIALS AND METHODS: the study was performed using conventional models of inflammation - carrageenan-induced edema of the limbs of rats. Content of interleukins IL-6 and IL-8 was determined by enzyme immunoassay (ELISA) according to a standard protocol Immunoassay kit IL-10, Biosourse (USA). The content of TNFα was determined by cytotoxic effect on sensitive mouse fibroblast line L-929. RESULTS AND DISCUSSION: it was shown that in the group of rats with carrageenan-induced edema contents of IL-6 increase in 2.8 times, IL-8 - in 5.6 times, and TNF-α - in 3 times compared to the control. Course influence of ultrasound contributed to reduction of the studied parameters. The content of IL-6 decreased in 1.7 times, IL-8 and TNF-α - in 1.6 times in comparison with inflammation.

Effect of fullerene C60 on ATPase activity and superprecipitation of skeletal muscle actomyosin.

Creation of new biocompatible nanomaterials, which can exhibit the specific biological effects, is an important complex problem that requires the use of last accomplishments of biotechnology. The effect of pristine water-soluble fullerene C60 on ATPase activity and superprecipitation reaction of rabbit skeletal muscle natural actomyosin has been revealed, namely an increase of actomyosin superprecipitation and Mg2+, Ca(2+)- and K(+)-ATPase activity by fullerene was investigated. We conclude that this finding offers a real possibility for the regulation of contraction-relaxation of skeletal muscle with fullerene C60.

[Interaction of beta-adrenergic agents with the plasma membranes of the myocardium]. / Vzaimodeistvie beta-adrenergicheskikh sredstv s plazmaticheskimi membranami miokarda.

The initial stage of interaction of beta-adrenomimetics and beta-adrenoblockers with specific membrane binding sites is characterized by different patterns of thermodynamic parameters. Administration of isadrin results in a decrease of entropy and enthalpy, which reflects the primary binding reaction and agonist-specific receptor isomerization to the conformational form activating adenylate cyclase. Anaprilin and alpheprol do not change the receptor conformation and participate only in the primary binding reaction which is marked by a decrease in enthalpy and a rise of entropy in the course of interaction of beta-adrenoblockers with specific membrane binding sites. The different patterns of thermodynamic parameters under the effect of beta-adrenomimetics and beta-adrenoblockers on plasma membranes are explained by the different influence of these substances on the mobility of membrane lipids, i.e. on the viscosity of plasma membranes.