Sex Differentiation of Trabecular Bone Structure Based on Textural Analysis of Pelvic Radiographs.

Objectives: The purpose of this paper is to assess the determination of male and female sex from trabecular bone structures in the pelvic region. The study involved analyzing digital radiographs for 343 patients and identifying fourteen areas of interest based on their medical significance, with seven regions on each side of the body for symmetry. Methods: Textural parameters for each region were obtained using various methods, and a thorough investigation of data normalization was conducted. Feature selection approaches were then evaluated to determine a small set of the most representative features, which were input into several classification machine learning models. Results: The findings revealed a sex-dependent correlation in the bone structure observed in X-ray images, with the degree of dependency varying based on the anatomical location. Notably, the femoral neck and ischium regions exhibited distinctive characteristics between sexes. Conclusions: This insight is crucial for medical professionals seeking to estimate sex dependencies from such image data. For these four specific areas, the balanced accuracy exceeded 70%. The results demonstrated symmetry, confirming the genuine dependencies in the trabecular bone structures.

Texture Analysis for the Bone Age Assessment from MRI Images of Adolescent Wrists in Boys.

Currently, bone age is assessed by X-rays. It enables the evaluation of the child's development and is an important diagnostic factor. However, it is not sufficient to diagnose a specific disease because the diagnoses and prognoses may arise depending on how much the given case differs from the norms of bone age. BACKGROUND: The use of magnetic resonance images (MRI) to assess the age of the patient would extend diagnostic possibilities. The bone age test could then become a routine screening test. Changing the method of determining the bone age would also prevent the patient from taking a dose of ionizing radiation, making the test less invasive. METHODS: The regions of interest containing the wrist area and the epiphyses of the radius are marked on the magnetic resonance imaging of the non-dominant hand of boys aged 9 to 17 years. Textural features are computed for these regions, as it is assumed that the texture of the wrist image contains information about bone age. RESULTS: The regression analysis revealed that there is a high correlation between the bone age of a patient and the MRI-derived textural features derived from MRI. For DICOM T1-weighted data, the best scores reached 0.94 R2, 0.46 RMSE, 0.21 MSE, and 0.33 MAE. CONCLUSIONS: The experiments performed have shown that using the MRI images gives reliable results in the assessment of bone age while not exposing the patient to ionizing radiation.

Textural Features of MR Images Correlate with an Increased Risk of Clinically Significant Cancer in Patients with High PSA Levels.

BACKGROUND: Prostate cancer, which is associated with gland biology and also with environmental risks, is a serious clinical problem in the male population worldwide. Important progress has been made in the diagnostic and clinical setups designed for the detection of prostate cancer, with a multiparametric magnetic resonance diagnostic process based on the PIRADS protocol playing a key role. This method relies on image evaluation by an imaging specialist. The medical community has expressed its desire for image analysis techniques that can detect important image features that may indicate cancer risk. METHODS: Anonymized scans of 41 patients with laboratory diagnosed PSA levels who were routinely scanned for prostate cancer were used. The peripheral and central zones of the prostate were depicted manually with demarcation of suspected tumor foci under medical supervision. More than 7000 textural features in the marked regions were calculated using MaZda software. Then, these 7000 features were used to perform region parameterization. Statistical analyses were performed to find correlations with PSA-level-based diagnosis that might be used to distinguish suspected (different) lesions. Further multiparametrical analysis using MIL-SVM machine learning was used to obtain greater accuracy. RESULTS: Multiparametric classification using MIL-SVM allowed us to reach 92% accuracy. CONCLUSIONS: There is an important correlation between the textural parameters of MRI prostate images made using the PIRADS MR protocol with PSA levels > 4 mg/mL. The correlations found express dependence between image features with high cancer markers and hence the cancer risk.

Multilayer outperforms single-layer slide scanning in AI-based classification of whole slide images with low-burden acid-fast mycobacteria (AFB).

Manual screening of Ziehl-Neelsen (ZN)-stained slides that are negative or contain rare acid-fast mycobacteria (AFB) is labor-intensive and requires repetitive refocusing to visualize AFB candidates under the microscope. Whole slide image (WSI) scanners have enabled implementation of AI to classify digital ZN-stained slides as AFB+ or AFB-. By default, these scanners acquire a single-layer WSI. However, some scanners can acquire a multilayer WSI with a z-stack and an extended focus image layer embedded. We developed a parameterized WSI classification pipeline to assess whether multilayer imaging improves ZN-stained slide classification accuracy. A CNN built into the pipeline classified tiles in each image layer to form an AFB probability score heatmap. Features extracted from the heatmap were then entered into a WSI classifier. 46 AFB+ and 88 AFB- single-layer WSIs were used for the classifier training. 15 AFB+ (with rare microorganisms) and 5 AFB- multilayer WSIs comprised the test set. Parameters in the pipeline included: (a) a WSI representation: z-stack of image layers, middle image layer (a single image layer equivalent) or an extended focus image layer, (b) 4 methods of aggregating AFB probability scores across the z-stack, (c) 3 classifiers, (d) 3 AFB probability thresholds, and (e) 9 feature vector types extracted from the aggregated AFB probability heatmaps. Balanced accuracy (BACC) was used to measure the pipeline performance for all parameter combinations. Analysis of Covariance (ANCOVA) was used to statistically evaluate the effect of each parameter on the BACC. After adjusting for other factors, a significant effect of the WSI representation (p-value < 1.99E-76), classifier type (p-value < 1.73E-21), and AFB threshold (p-value = 0.003) was observed on the BACC. The feature type had no significant effect (p-value = 0.459) on the BACC. WSIs represented by the middle layer, extended focus layer and the z-stack followed by the weighted averaging of AFB probability scores were classified with the average BACC of 58.80%, 68.64%, and 77.28%, respectively. The multilayer WSIs represented by the z-stack with the weighted averaging of AFB probability scores were classified by a Random Forest classifier with the average BACC of 83.32%. Low classification accuracy of WSIs represented by the middle layer suggests that they contain fewer features permitting identification of AFB than the multilayer WSIs. Our results indicate that single-layer acquisition can introduce a bias (sampling error) into the WSI. This bias can be mitigated by the multilayer or the extended focus acquisitions.

Performance of QR Code Detectors near Nyquist Limits.

For the interacting with real world, augmented reality devices need lightweight yet reliable methods for recognition and identification of physical objects. In that regard, promising possibilities are offered by supporting computer vision with 2D barcode tags. These tags, as high contrast and visually well-defined objects, can be used for finding fiducial points in the space or to identify physical items. Currently, QR code readers have certain demands towards the size and visibility of the codes. However, the increase of resolution of built-in cameras makes it possible to identify smaller QR codes in the scene. On the other hand, growing resolutions cause the increase to the computational effort of tag location. Therefore, resolution reduction in decoders is a common trade-off between processing time and recognition capabilities. In this article, we propose the simulation method of QR codes scanning near limits that stem from Shannon's theorem. We analyze the efficiency of three publicly available decoders versus different size-to-sampling ratios (scales) and MTF characteristics of the image capture subsystem. The MTF we used is based on the characteristics of real devices, and it was modeled using Gaussian low-pass filtering. We tested two tasks-decoding and locating-and-decoding. The findings of the work are several-fold. Among others, we identified that, for practical decoding, the QR-code module should be no smaller than 3-3.5 pixels, regardless of MTF characteristics. We confirmed the superiority of Zbar in practical tasks and the worst recognition capabilities of OpenCV. On the other hand, we identified that, for borderline cases, or even below Nyquist limit where the other decoders fail, OpenCV is still capable of decoding some information.

Differential Diagnosis of Cysts and Granulomas Supported by Texture Analysis of Intraoral Radiographs.

The aim of this study was to evaluate whether textural analysis could differentiate between the two common types of lytic lesions imaged with use of radiography. Sixty-two patients were enrolled in the study with intraoral radiograph images and a histological reference study. Full textural analysis was performed using MaZda software. For over 10,000 features, logistic regression models were applied. Fragments containing lesion edges were characterized by significant correlation of structural information. Although the input images were stored using lossy compression and their scale was not preserved, the obtained results confirmed the possibility of distinguishing between cysts and granulomas with use of textural analysis of intraoral radiographs. It was shown that the important information distinguishing the aforementioned types of lesions is located at the edges and not within the lesion.

Evaluation of Keypoint Descriptors for Flight Simulator Cockpit Elements: WrightBroS Database.

The goal of the WrightBroS project is to design a system supporting the training of pilots in a flight simulator. The desired software should work on smart glasses supplementing the visual information with augmented reality data, displaying, for instance, additional training information or descriptions of visible devices in real time. Therefore, the rapid recognition of observed objects and their exact positioning is crucial for successful deployment. The keypoint descriptor approach is a natural framework that is used for this purpose. For this to be applied, the thorough examination of specific keypoint location methods and types of keypoint descriptors is required first, as these are essential factors that affect the overall accuracy of the approach. In the presented research, we prepared a dedicated database presenting 27 various devices of flight simulator. Then, we used it to compare existing state-of-the-art techniques and verify their applicability. We investigated the time necessary for the computation of a keypoint position, the time needed for the preparation of a descriptor, and the classification accuracy of the considered approaches. In total, we compared the outcomes of 12 keypoint location methods and 10 keypoint descriptors. The best scores recorded for our database were almost 96% for a combination of the ORB method for keypoint localization followed by the BRISK approach as a descriptor.

Application of Photo Texture Analysis and Weather Data in Assessment of Air Quality in Terms of Airborne PM10 and PM2.5 Particulate Matter.

The study was undertaken in Krakow, which is situated in Lesser Poland Voivodeship, where bad PM10 air-quality indicators occurred on more than 100 days in the years 2010-2019. Krakow has continuous air quality measurement in seven locations that are run by the Province Environmental Protection Inspectorate. The research aimed to create regression and classification models for PM10 and PM2.5 estimation based on sky photos and basic weather data. For this research, one short video with a resolution of 1920 × 1080 px was captured each day. From each film, only five frames were used, the information from which was averaged. Then, texture analysis was performed on each averaged photo frame. The results of the texture analysis were used in the regression and classification models. The regression models' quality for the test datasets equals 0.85 and 0.73 for PM10 and 0.63 for PM2.5. The quality of each classification model differs (0.86 and 0.73 for PM10, and 0.80 for PM2.5). The obtained results show that the created classification models could be used in PM10 and PM2.5 air quality assessment. Moreover, the character of the obtained regression models indicates that their quality could be enhanced; thus, improved results could be obtained.

A CNN-based active learning framework to identify mycobacteria in digitized Ziehl-Neelsen stained human tissues.

Tuberculosis is the most common mycobacterial disease that affects humans worldwide. Rapid and reliable diagnosis of mycobacteria is crucial to identify infected individuals, to initiate and monitor treatment and to minimize or prevent transmission. Microscopic identification of acid-fast mycobacteria (AFB) in tissue sections is usually accomplished by examining Ziehl-Neelsen (ZN) stained slides in which AFB appear bright red against the blue background. Because the ZN-stained slides require time consuming and meticulous screening by an experienced pathologist, our team developed a machine learning pipeline to classify digitized ZN-stained slides as AFB-positive or AFB-negative. The pipeline includes two convolutional neural network (CNN) models to recognize tiles containing AFB, and a logistic regression (LR) model to classify slides based on features from AFB-probability maps assembled from the CNN tile-based classification results. The first CNN was trained using tiles from 6 AFB-positive and 8 AFB-negative slides, and the second CNN was trained using the initial tile set expanded by additional tiles from 19 AFB-negative slides selected within an active learning framework. When evaluated on a separate set of tiles, the two CNNs yielded F1 scores of 99.03% and 98.75%, respectively, and were used to classify tiles in a separate set of 134 slides (46 AFB-positive and 88 AFB-negative). The classification yielded two AFB-probability maps, one for each CNN. The LR model was then 10-fold cross-validated using the average of feature vectors extracted from the AFB-probability maps generated by each CNN. The feature vector consisted of seven features of the AFB-probability map histogram and the positive tile rate (PTR). The sensitivity (87.13%), specificity (87.62%) and F1 (80.18%) achieved by this model were superior to the baseline performance of PTR-based separation of slides that yielded F1 scores of 73.13% and 66.67% in the AFB-probability maps outputted by the CNN trained within the active learning framework and the CNN trained only on the initial set of slides, respectively. Our CNNs outperformed several recently published models for AFB detection. Active learning induced robust learning of features by the CNN and led to improved LR classification performance of slides. In the 52 AFB-positive slides used in the pipeline development, the AFB were infrequent, predominantly single and only rarely found in small clusters. Our pipeline can classify slides and visualize suspected AFB-positive areas in each slide, and thus potentially facilitate evaluation of ZN-stained tissue sections for AFB.

Caries detection enhancement using texture feature maps of intraoral radiographs.

OBJECTIVES: Dental caries are caused by tooth demineralization due to bacterial plaque formation. However, the resulting lesions are often discrete and thus barely recognizable in intraoral radiography images. Therefore, more advanced detection techniques are in great demand among dentists and radiographers. This study was performed to evaluate the performance of texture feature maps in the recognition of discrete demineralization related to caries plaque formation. METHODS: Digital intraoral radiology image analysis protocols incorporating first-order features (FOF), co-occurrence matrices, gray tone difference matrices, run-length matrices (RLM), local binary patterns (LBP), and k-means clustering (CLU) were used to transform the digital intraoral radiology images of 10 patients with confirmed caries, which were retrospectively reviewed in a dental clinic. The performance of the resulting texture feature maps was compared with that of radiographic images by radiologists and dental specialists. RESULTS: Significantly improved detection of caries spots was achieved by employing the CLU and FOF texture feature maps. The caries-affected area with sharp margins was well defined using the CLU approach. A pseudo-three-dimensional effect was observed in outlining the demineralization zones inside the cavity with the FOF 5 protocol. In contrast, the LBP and RLM techniques produced less satisfactory results with unsharp edges and less detailed depiction of the lesions. CONCLUSIONS: This study illustrated the applicability of texture feature maps to the recognition of demineralized spots on the tooth surface debilitated by caries and identified the best performing techniques.

CAS: Cell Annotation Software - Research on Neuronal Tissue Has Never Been so Transparent.

CAS (Cell Annotation Software) is a novel tool for analysis of microscopic images and selection of the cell soma or nucleus, depending on the research objectives in medicine, biology, bioinformatics, etc. It replaces time-consuming and tiresome manual analysis of single images not only with automatic methods for object segmentation based on the Statistical Dominance Algorithm, but also semi-automatic tools for object selection within a marked region of interest. For each image, a broad set of object parameters is computed, including shape features and optical and topographic characteristics, thus giving additional insight into data. Our solution for cell detection and analysis has been verified by microscopic data and its application in the annotation of the lateral geniculate nucleus has been examined in a case study.

Influence of applied corneal endothelium image segmentation techniques on the clinical parameters.

The corneal endothelium state is verified on the basis of an in vivo specular microscope image from which the shape and density of cells are exploited for data description. Due to the relatively low image quality resulting from a high magnification of the living, non-stained tissue, both manual and automatic analysis of the data is a challenging task. Although, many automatic or semi-automatic solutions have already been introduced, all of them are prone to inaccuracy. This work presents a comparison of four methods (fully-automated or semi-automated) for endothelial cell segmentation, all of which represent a different approach to cell segmentation; fast robust stochastic watershed (FRSW), KH method, active contours solution (SNAKE), and TOPCON ImageNET. Moreover, an improvement framework is introduced which aims to unify precise cell border location in images pre-processed with differing techniques. Finally, the influence of the selected methods on clinical parameters is examined, both with and without the improvement framework application. The experiments revealed that although the image segmentation approaches differ, the measures calculated for clinical parameters are in high accordance when CV (coefficient of variation), and CVSL (coefficient of variation of cell sides length) are considered. Higher variation was noticed for the H (hexagonality) metric. Utilisation of the improvement framework assured better repeatability of precise endothelial cell border location between the methods while diminishing the dispersion of clinical parameter values calculated for such images. Finally, it was proven statistically that the image processing method applied for endothelial cell analysis does not influence the ability to differentiate between the images using medical parameters.