Effect of dietary ß-mannanase supplementation on broiler performance.

This study was conducted to investigate the effect of graded levels of ß-mannanase supplementation in broiler diets on growth performance, energy digestibility, and lesion scores in d-old birds fed mash, corn-soybean meal-based diets and raised to 42 d. Five dietary treatments were investigated: 1) positive control diet (PC) containing standard energy; 2) negative control (NC) with 100 kcal/kg diet reduction in AME compared to PC; 3) NC supplemented with 30 U/g ß-mannanase (NC + 30 U); 4) NC supplemented with 60 U/g ß-mannanase (NC + 60 U); and 5) NC supplemented with 90 U/g ß-mannanase (NC + 90 U). Each treatment had 6 replicate pens with 52 chicks per replicate. Data was analyzed using 1-way ANOVA, and means were separated by LSMEANS. Reduction of 100 kcal/kg feed (NC) resulted in an overall body weight gain reduction of 51 g (P < 0.05) and feed conversion loss of approximately 4 points (P < 0.05) compared to PC at 42 d of age. At the same time, supplementing ß-mannanase at 60 and 90 U/g improved growth performance parameters compared to NC, while 30 U/g did not result in significant improvements beyond NC; body weight gain was improved (P < 0.05) by 87, and 106 g when ß-mannanase was supplemented at 60 and 90 U/g, respectively, compared to NC. This corresponded to an improvement by 6 and 7 points in feed conversion for 60 and 90 U/g supplementation, respectively, compared to NC. Furthermore, AMEn was improved (P < 0.05) by 15, 97, and 116 kcal/kg at 42 d when ß-mannanase was added to NC at 30, 60, and 90 U/g, respectively. Digesta viscosity measured at 42 d was decreased (P < 0.05) by ß-mannanase supplementation of 60 and 90 U/g, compared to NC, while 42 d lesion scores were improved (P < 0.05) by ß-mannanase supplementation compared to NC. Data demonstrated that dietary supplementation of ß-mannanase improved growth performance, energy digestibility, and reduced viscosity and lesion scores when supplemented with diets with a reduced energy content of 100 kcal/kg compared to a standard energy diet.

Effect of endo-1,4-beta-xylanase supplementation to low-energy diets on performance, blood constituents, nutrient digestibility, and gene expressions related growth of broiler chickens.

The presence of soluble and insoluble non-starch polysaccharides (NSP) was reported to reduce nutrient utilisation, and adversely impact the broilers' growth performance; accordingly, NSP-degrading enzymes are essential supplements to cereal-based diets. Therefore, the current trial was conducted to characterise the impacts of supplemental xylanase (Xyl) to diets with low-ME levels on performance, carcass traits, blood parameters, nutrient digestibility and some genes expressions in broiler chickens. A total of 600 1-day-old Ross 308 male broiler chicks were randomly assigned to 6 treatments with 10 replications of 10 birds each per group in a completely randomised design. The 6 treatments were as follow: (1) basal diets with balanced ME content served as control (positive control, PC), (2) low-energy diet (negative control 1 [NC1]; ME content reduced by 70 kcal/kg compared with PC), (3) low-energy diet (negative control 2 [NC2]; ME content reduced by 140 kcal/kg compared with PC), (4) NC1 + 100 g/ton xylanase (NC1 + 100Xyl), (5) NC2 + 100 g/ton xylanase (NC2 + 100Xyl), and (6) NC1 + 50 g/ton xylanase (NC1 + 50Xyl). At the end of the experiment (35 days of age), the reduction of energy in the NC diets yielded lower live body weight (BW) and total body weight gain (BWG) (p ˂ 0.001); however, it significantly increased feed intake (p ˂ 0.05), leading to worst feed conversion ratio (FCR) and European production efficiency factor (EPEF) (p ˂ 0.01) than PC. There was non-significant variation in final BW, BWG, FCR, or EPEF between the PC group and the NC groups supplemented with Xyl. Carcass yield, gizzard, liver and, muscle relative weights were not influenced by dietary treatments; while broilers fed diet with low-energy diets with or without Xyl addition had lower abdominal fat (p ˂ 0.01) than PC. Furthermore, broilers fed on low-ME diets supplemented with Xyl showed a reduction in plasma total cholesterol (p ˂ 0.05) and low density lipoprotein (p ˂ 0.01) levels. Greater antibody titre against Newcastle disease (p ˂ 0.05) was recorded in the NC1 + 100Xyl and NC2 + 100Xyl groups. The addition of Xyl to low-energy diets significantly improved (p ˂ 0.05) fibre digestibility compared to the PC group. Moreover, enzyme supplementation increased muscle total lipids content and decreased muscle thiobarbituric acid retroactive substance content. In addition, enzyme supplementation increased gene expression related to growth and gene expression related to fatty acid synthesis. It was concluded that a low-ME diet might diminish broiler performance, whereas Xyl supplementation to low-ME diets beneficially affected growth performance, abdominal fat percentage, nutrient digestibility and immunity for broilers, and gene expressions related to growth and fatty acid synthesis in broiler chickens fed low-energy diets.

Evaluation of Dioxin/Furan and Elements in Poultry from Zarqa Governorate, Jordan.

This study is the first to determine the concentration for 17 congeners of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) and element contamination in poultry that is close to petroleum refinery at Al-Hashemiya Municipality, Zarqa Governorate, Jordan. Ten different samples (chicken) were collected to cover ten different locations of poultry farms in Al-Hashemiya Municipality. These locations are considered polluted areas as a result of exhaust gases produced from the refinery. The 17 PCDD/Fs congeners and elements of Pb, Cd, As, Zn, Cu, Se, Hg, Cr, and Ni were determined for three parts of each sample (liver, muscle, and gizzard). All samples were analyzed for PCDD/Fs after a Soxhlet extraction procedure and cleanup by column chromatography; then, all compounds were identified and determined using GC-MS techniques. The elements were analyzed after digestion and measured using an inductively coupled plasma optical emission spectrometer (ICP-OES) and validated with the Lab Mix24 RM NCS ZC73016 reference material. The highest total sum concentration of PCDD/Fs was found in liver samples to be 214.07 ng/kg (dry weight), while the highest sum of toxicity equivalent to PCDD/Fs of 22.54 ng TEQ/kg was found in gizzard samples. For element concentrations, the highest total sum of 16.89 mg/kg (dry weight) was found in liver samples. The concentration level of the elements of Se, Hg, Cr, and Ni for all parts of the chicken was within an acceptable range according to Jordanian standards and therefore the measured level of heavy and trace elements in the poultry samples (chicken) does not pose a danger to public health. The chickens found in poultry farms near the refinery are more likely to contain a higher concentration of PCDD/Fs congeners due to exhaust gas exposure.

Growth performance and carcass traits of two commercial broiler strains fed diet supplemented with essential oils.

The current study aimed to evaluate the efficacy of using essential oils (EOs) on growth, carcass, and meat quality traits of two commercial broiler strains raised to 35 days of age. A total of 384 chicks were obtained upon hatching from a local hatchery (192 Indian River and 192 Hubbard). Birds were allocated randomly according to their strain into three groups: control, EOs, and EOs grower. Body weight was recorded at the beginning of the trial and then at the end of each phase diet as well as for the feed intake. Sixteen birds from the combination of each strain-essential oil were chosen randomly to evaluate carcass characteristics at the end. Hubbard consumed more feed during the grower stage (p = 0.02) and overall (p = 0.002) compared to Indian River. Carcass cuts percentages were affected by strain (p < 0.01). Shear force was lower for the Hubbard (p = 0.002). Essential oils showed a significant effect on cooking loss (p = 0.03). A significant strain by essential oil interactions was obtained for cooking loss, shear force, and meat redness (color coordinate ∗a) Cooking loss was greater for the Indian river with the EOs grower treatment. Briefly, growth and carcass traits were affected by strain. The inclusion of EOs had slightly improved meat quality traits.

Live Performance and Microbial Load Modulation of Broilers Fed a Direct-Fed Microbials (DFM) and Xylanase Combination.

The animal industry, which focuses on producing protein for human consumption, is continuously seeking solutions that can enhance both animal performance and health at a low cost. Several feed additives are currently being used to improve the nutritive value of feed as well as replacing the subtherapeutic levels of antibiotic growth promoters (AGP). This study was designed to investigate the effect of a feed additive that is a blend of multi-strain Bacillus spp. probiotics and a xylanase in a 2 × 2 factorial dietary treatments design, testing two levels of the feed additive blend (0 and 100 g/MT) and two cereal grain types (corn and wheat) on live performance, gut lesions, environmental Clostridium perfringens load, and pathogen load in the digesta of broiler chickens (E. tenella, total aerobic count cells (APC), E. coli, and C. perfringens). Day-old chicks were randomly placed in 10 replicate pens per treatment with 52 birds per replicate and grown to 42 d of age. Data were analyzed by two-way ANOVA. At 42 d, birds fed EnzaPro were heavier (p < 0.0004) than unsupplemented birds. An improvement in FCR (p = 0.03) was observed from 1 to 42 d by approximately two points in both corn- and wheat-based diets supplemented with EnzaPro. In wheat-based diets, supplementing EnzaPro reduced (p < 0.0001) a 21 d lesion score of intestines with a further reduction (p < 0.02) at 42 d. EnzaPro reduced (p < 0.03) litter moisture by approximately 1% compared to non-supplemented EnzaPro in both corn- and wheat-based diets. Pathogen load in digesta (C. perfringens, E. tenella, APC, and E. coli) was reduced (p < 0.0002) when EnzaPro was supplemented in diets. It can be concluded that EnzaPro (a blend of DFM Bacillus spp (1 × 105 CFU/g feed) and xylanase (10 XU/g feed)) may be used in both corn- and wheat-based diets to improve the performance and gut health of broilers.

The Effect of a Modified GH11 Xylanase on Live Performance, Gut Health, and Clostridium perfringens Excretion of Broilers Fed Corn-Soy Diets.

Xylanase enzymes and other feed additives are being used more commonly in poultry feed to reduce feed cost, improve performance, and maintain gut health. Five corn-soy-based dietary treatments were designed to compare the effect of different inclusion levels of high-efficiency GH11 xylanase on live performance, gut lesions, and Clostridium perfringens excretion in littler samples of broiler chickens. Diets were the standard diet (positive control; PC); a diet of reduced energy by 130 kcal/kg diet (negative control; NC); NC with xylanase at 10 XU/g of feed (NC + 10); NC with xylanase at 12.5 XU/g of feed (NC + 12.5); NC with xylanase at 15 XU/g of feed (NC + 15). Data were analyzed with one-way ANOVA. At 42 d, birds fed NC + 12.5 and NC + 15 were heavier (P < 0.05) than NC and comparable improvement to birds fed PC. Significant Improvement in FCR (P = 0.0001) was observed from 1 to 42 d for NC + 12.5 and NC + 15 compared with NC. Supplementation of xylanase reduced (P < 0.005) 21 d intestinal lesion score at 21 d with further improvement (P < 0.0001) at 42 d. NC + 15 reduced lesion scores by 24% compared with NC. Xylanase supplementations reduced litter C. perfringens cell forming unit per gram (CFU/g) compared with NC with the highest reduction of NC + 15 treatment by ~27%. In conclusion, xylanase can be included in reduced-energy diets up to 15 XU/g of feed to improve live performance, energy digestibility, and reduce intestinal lesion scores in broilers.

Xylanase and Direct-Fed Microbials (DFM) Potential for Improvement of Live Performance, Energy Digestibility, and Reduction of Environmental Microbial Load of Broilers.

The challenge of identifying alternatives to subtherapeutic levels of antibiotic growth promoters (AGP) in animal feed has led to increased interest in feed additives such as exogenous enzymes and direct-fed microbials (DFM). Six corn soy-based dietary treatments were designed to investigate the effect of high-efficiency xylanase alone, Bacillus spp. probiotics alone, and their combination vs. a commonly used antibiotic growth promoter (bacitracin methylene disalicylate; BMD) on live performance and environmental Clostridium perfringens load of broiler chickens with eight replicate pens per treatment. Diets were as follows: standard diet (positive control; PC); 130 kcal/kg reduced-energy diet (negative control; NC); NC with xylanase (NC + Xy); NC with probiotics (NC + Pro); NC with xylanase and probiotics mix (NC + XyPro); and NC with BMD (NC + BMD). Data were analyzed as one-way ANOVA. At 35 and 42 days, birds fed with NC + XyPro and NC + BMD were heavier (P < 0.05) than birds fed with NC. Improvement in feed conversion ratio (FCR) (P = 0.0001) was observed from 1 to 42 days by ~3 points in both NC + XyPro and NC + BMD compared to NC. The NC + XyPro reduced lesion scores by 66% compared to PC and NC. Litter C. perfringens cell count was reduced by ~16% with supplementation of XyPro or BMD. It can be concluded that a blend of xylanase (10 XU/g feed) and Bacillus spp. [1 × 105 colony forming units (CFU)/g feed] can be used as an alternative to AGP in low-energy broiler diets.

Fattening performance and carcass traits of Baladi and Shami-Baladi kids.

BACKGROUND AND AIM: Goat is considered very important ruminant animals that are raised in Jordan; however, the production of the local breeds is low to moderate, and thus, some genetic improvement programs could have a positive effect on the performance of the local breeds. The objective of this study was to investigate the effect of crossing Baladi (BB) goat with Shami. MATERIALS AND METHODS: A total of 30 newly weaned goat kids (15 BB and 15Shami-Baladi [SB] kids) were evaluated for growth performance and carcass traits. The trial lasted for 77 days (7 days for adaptation and 70 days for data collection). Feed intake was measured on a daily basis; body weight of kids was measured at the beginning of the study and biweekly thereafter. At the end of the trial, kids were slaughtered to examine carcass traits. RESULTS: Dry matter intake, initial weight, final weight, and average daily gain (ADG) were not affected by kid's breed type. In addition, breed type of kids showed no significant effect on feed efficiency (feed:gain). Hot and cold carcass weight and dressing percentage were also not affected by kid's breed type. Kid breed type had no significant effect on shoulder, rack, and legs percentages. However, SB kids had a higher loin cut percentage compared to BB kids. In addition, Musculus longissimus depth was higher for SB kids. CONCLUSION: Results of this study indicate that crossing SB goat breeds did not have a significant effect on either growth performance or carcass traits. Further studies using reciprocal cross may reveal different results.

Effect of Limestone Particle Size and Potassium Supplementation on Growth Performance, Blood Physiology, and Breast Muscle Myopathy of Male Broiler Chickens.

The experiment investigated the effects of limestone particle size and dietary potassium (K) on live performance, blood physiology, and muscle myopathies in broilers raised to 35 days of age. A total of 384 Ross male broilers were placed in 24 floor pens and fed four diets during the starter (0-16 days of age) and grower (17-33 days of age) periods containing two limestone particle sizes (fine: 0.2 mm and coarse: 0.9 mm), and amended with either 0% basal K (K-) or 0.2% added dietary K (K+) as potassium carbonate to complete the 2 × 2 factorial arrangement. Live performance was measured from 1-33 days of age. Blood physiology, woody breast (WB), and white striping (WS) scores were measured at 35 days of age. The K+ dietary treatment reduced (P < 0.05) feed intake and BWG when compared to K- during the starter and grower period. The K+ dietary treatment decreased blood Na (mmol/L), blood glucose (mg/dl), ionized blood Ca (mg/dl), TCO2 (mmol/L), blood HCO3 (mmol/L), and base excess in extracellular fluid (mmol/L) when compared to K- birds of similar body weight at 35 days of age (P ≤ 0.05). Fine limestone diets tended to reduce WB scores (3.0 vs. 2.59) when compared to coarse limestone diets at 35 days of age (P = 0.08). This study demonstrated that using 0.2% of K as potassium carbonate did not negatively affect FCR even though FI and BWG were reduced. Furthermore, fine limestone has the potential to reduce WB in breast muscle tissues; however, further research is needed to confirm these outcomes.

The Potential Use of Layer Litter in Awassi Lamb Diet: Its Effects on Carcass Characteristics and Meat Quality.

Carcass parameters and meat quality in lambs that consumed diets having layer hen litter (LL) were evaluated in a complete randomized study. Forty-two lambs were allocated equally (14 lambs/treatment diet) into one of three iso-nitrogenous diets for 75 days. To partially replace soybean meal and barley, LL was given at 0 (LL0), 150 (LL150), or 300 g/kg (LL300) of dietary dry matter (DM). At the termination of the trial, the characteristics of carcasses (hot and cold carcass weight, dressing percentage, and carcass cuts) and meat quality (Musculus longissimus linear dimensions, ultimate pH, cooking loss, water holding capacity (WHC), shear force (SF), color coordinates) were measured after slaughtering all lambs. Longissimus muscle weight was greatest (p < 0.05) for the LL150. For the dissected loin, intermuscular fat content was lowest for the LL0 diet. However, subcutaneous fat content was lower (p < 0.05) in the LL300 diet than LL0 and LL150 diets. Rib fat depth and Musculus longissimus area were greater (p < 0.05) for LL150 than L0. No differences were found in meat pH or color parameters among treatments but WHC and SF were lower in L0 lambs than in lambs fed LL containing diets. Cooking loss was greater for the LL300 diet than the LL0 diet. In summary, quality of meat and carcasses data indicate the possibility of inclusion of LL up to 300 g/kg DM to growing Awassi lambs.

Thermotolerance of Broiler Chicks Ingesting Dietary Betaine and/or Creatine.

The present study aimed to assess the effect of dietary betaine (B) and/or creatine (C) on performance and thermoregulatory responses of broiler chicks. Indian River broiler chicks, fitted with compact thermosensors, were reared to market age (five weeks). The chicks were randomly distributed into four treatment groups, in a 2 × 2 factorial arrangement of treatments-basal control diet (Control group: CONT; B-/C-); 1 g betaine/kg feed (Betaine group: BETA; B+/C-), 1.2 g creatine monohydrate/kg feed (Creatine group: CRET; B-/C+), and combination (Betaine and Creatine group: COMB; B+/C+) of both supplements. At 31 days of age, 20 chicks from each group were exposed to acute heat stress (A-HS) for 3 h (34.45 ± 0.20 °C), and hemogramic profiles were screened before and after. Performance parameters (feed intake, body weight gain, and feed conversion ratio) were reported on a weekly basis, and carcass meat quality was evaluated at the end of experiment. Redness of breast was higher due to B and C treatments separately than the CONT group (B by C interaction; p < 0.05). Compared to the CONT, dietary supplements alleviated hyperthermia responses, with B alone being more efficient than C or COMB treatments. The mitigation of hyperthermia is likely mediated by enhancement of water balance indicators. Although not efficient in improving growth performance, dietary B and/or C are efficient in improving thermophysiological performance and survival of finishing broiler chicks under A-HS.