RESUMO
The microbial communities residing in the mosquito midgut play a key role in determining the outcome of mosquito pathogen infection. Elizabethkingia anophelis, originally isolated from the midgut of Anopheles gambiae possess a broad-spectrum antiviral phenotype, yet a gap in knowledge regarding the mechanistic basis of its interaction with viruses exists. The current study aims to identify pathways and genetic factors linked to E. anophelis antiviral activity. The understanding of E. anophelis antiviral mechanism could lead to novel transmission barrier tools to prevent arboviral outbreaks. We utilized a non-targeted multi-omics approach, analyzing extracellular lipids, proteins, metabolites of culture supernatants coinfected with ZIKV and E. anophelis. We observed a significant decrease in arginine and phenylalanine levels, metabolites that are essential for viral replication and progression of viral infection. This study provides insights into the molecular basis of E. anophelis antiviral phenotype. The findings lay a foundation for in-depth mechanistic studies.
Assuntos
Flavobacteriaceae , Zika virus , Zika virus/fisiologia , Animais , Flavobacteriaceae/metabolismo , Flavobacteriaceae/genética , Anopheles/virologia , Anopheles/microbiologia , Infecção por Zika virus/virologia , Antivirais/farmacologia , Antivirais/metabolismo , Replicação Viral , Fenilalanina/metabolismo , Arginina/metabolismo , MultiômicaRESUMO
A comparative study of the anticonvulsant properties of root and stem extracts of Calliandra portoricensis was carried out in mice. The convulsion models were pentylenetetrazole- and electroshock-induced convulsions. The results showed that the aqueous extracts of both root and stem possess anticonvulsant activity when given intraperitoneally. An alkaloidal extract did not show any anticonvulsant action up to 316 mg/kg i.p.
Assuntos
Anticonvulsivantes/farmacologia , Plantas Medicinais/análise , Animais , Eletrochoque , Camundongos , Camundongos Endogâmicos , Nigéria , Pentilenotetrazol/toxicidade , Extratos Vegetais/análise , Extratos Vegetais/farmacologiaRESUMO
The anticonvulsant effect of the volatile oil extracted from the fresh fruits of Tetrapleura tetraptera was investigated in mice. The results obtained show that the fresh oil given intraperitoneally offers some protection against leptazol-induced convulsions. A dose of 0.4 ml of the oil per mouse protected 78% of the animals when administered 30 min prior to leptazol. While 0.6 ml offered no protection from death, the onset of convulsions and the average time of death was prolonged.
Assuntos
Anticonvulsivantes , Óleos Voláteis/uso terapêutico , Óleos de Plantas/uso terapêutico , Plantas Medicinais , Convulsões/tratamento farmacológico , África Ocidental , Animais , Feminino , Frutas , Masculino , Camundongos , Pentilenotetrazol/farmacologia , Convulsões/induzido quimicamente , Convulsões/prevenção & controleRESUMO
The occurrence of quaternary alkaloids in TINOSPORA (and PARABAENA) species (Menispermaceae) has been studied. The main components were generally the protoberberine bases berberine and palmatine, with jatrorrhizine an occasional minor constituent, and the aporphine base magnoflorine. Choline was also often present. Only magnoflorine was detected in the PARABAENA material examined.