RESUMO
Six virus isolations were made from Culicoides biting midges and blood samples of sentinel cattle in Kagoshima and Miyazaki Prefectures, the southern part of Japan, in 2002. Serological and genetical tests identified these viruses as isolates of Shamonda virus (SHAV), which belongs to the Simbu group of the genus Orthobunyavirus of the family Bunyaviridae. Initially, SHAV was isolated from cattle and Culicoides biting midges in Nigeria in the 1960s, and its presence has not been reported until this study. The present results indicate a wider distribution of SHAV than previously assumed.
Assuntos
Infecções por Bunyaviridae/veterinária , Doenças dos Bovinos/virologia , Ceratopogonidae/virologia , Orthobunyavirus/isolamento & purificação , Animais , Antígenos Virais/imunologia , Infecções por Bunyaviridae/virologia , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/prevenção & controle , Japão , Dados de Sequência Molecular , Testes de Neutralização , Orthobunyavirus/genética , Orthobunyavirus/imunologia , Filogenia , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido NucleicoRESUMO
The Limulus amoebocyte lysate (LAL) test, a simple and rapid method, was applied for the evaluation of bacterial contamination of carcasses in a slaughterhouse. Twenty-five pork carcasses were examined for bacterial contamination, and we evaluated the correlation between the LAL test and the methods usually employed for detecting indicator organisms of total viable cells, coliforms or Staphylococcus aureus. The reciprocal of the highest dilution found to be positive was used as the LAL index. A high correlation was observed between the LAL index and the number of coliforms, but not between the index and the number of other organisms. The LAL test was accomplished within 2 hr after sampling, and thus may serve as a useful tool for the substitute indicator of coliforms among bacterial contamination in carcasses.
