RESUMO
The incidence of human papillomavirus (HPV) DNA in archival formalin-fixed, paraffin-embedded tissue sections from verrucous carcinoma of the larynx was determined using polymerase chain reaction (PCR) with consensus primers and by in situ hybridization designed to detect HPV types 6/11, 16/18, 31/33/35. HPV DNA was detected in 17 (85%) of 20 tissue samples by PCR; none of the 20 samples were positive for the seven genotype types tested by in situ hybridization. PCR is a valuable tool to detect HPV and therefore will significantly clarify the importance of HPV in squamous mucosal disorders.
Assuntos
Carcinoma Papilar/microbiologia , DNA Viral/análise , Neoplasias Laríngeas/microbiologia , Papillomaviridae/genética , Reação em Cadeia da Polimerase , Adulto , Idoso , Idoso de 80 Anos ou mais , Sondas de DNA , Feminino , Amplificação de Genes , Genoma Viral , Globinas/genética , Humanos , Masculino , Pessoa de Meia-Idade , Hibridização de Ácido Nucleico , Papillomaviridae/classificação , Papillomaviridae/isolamento & purificação , Estudos RetrospectivosRESUMO
The major advantage of intranasal endoscopy is that it allows a magnified view of the osteomeatal region and the frontal recess. Performing intranasal ethmoidectomy with nasal endoscopes may be hazardous, particularly in the hands of inexperienced surgeons. The use of 2-power loops for performing intranasal ethmoidectomy has several advantages, including direct binocular vision and visualization of external anatomic landmarks. The use of loops does not preclude the use of endoscopes.
