RESUMO
The development of oil exploration activities and an increase in shipping in Arctic areas have increased the risk of oil spills in this cold marine environment. The objective of this experimental study was to assess the effect of biostimulation on microbial community abundance, structure, dynamics, and metabolic potential for oil hydrocarbon degradation in oil-contaminated Arctic seawater. The combination of amplicon-based and shotgun sequencing, together with the integration of genome-resolved metagenomics and omics data, was applied to assess microbial community structure and metabolic properties in naphthenic crude oil-amended microcosms. The comparison of estimates for oil-degrading microbial taxa obtained with different sequencing and taxonomic assignment methods showed substantial discrepancies between applied methods. Consequently, the data acquired with different methods was integrated for the analysis of microbial community structure, and amended with quantitative PCR, producing a more objective description of microbial community dynamics and evaluation of the effect of biostimulation on particular microbial taxa. Implementing biostimulation of the seawater microbial community with the addition of nutrients resulted in substantially elevated prokaryotic community abundance (103-fold), a distinctly different bacterial community structure from that in the initial seawater, 1.3-fold elevation in the normalized abundance of hydrocarbon degradation genes, and 12% enhancement of crude oil biodegradation. The bacterial communities in biostimulated microcosms after four months of incubation were dominated by Gammaproteobacterial genera Pseudomonas, Marinomonas, and Oleispira, which were succeeded by Cycloclasticus and Paraperlucidibaca after eight months of incubation. The majority of 195 compiled good-quality metagenome-assembled genomes (MAGs) exhibited diverse hydrocarbon degradation gene profiles. The results reveal that biostimulation with nutrients promotes naphthenic oil degradation in Arctic seawater, but this strategy alone might not be sufficient to effectively achieve bioremediation goals within a reasonable timeframe.
RESUMO
The biotransformation of hydrocarbon compounds in seawater at sub-zero temperature has gained research interest in recent years with the most focus placed on temperatures around 0°C. In this study, biotransformation of dispersed diesel at sub-zero temperatures (-2°C to -6°C) in artificial brines, prepared by adding Instant Ocean salt to natural seawater to increase salinity, is examined. The oil was pre-mixed with dispersant Finasol 51 to prepare the dispersed oil at 2â mgâ l-1. The native microorganisms in sub-arctic seawater were able to adapt to high salinity and lower temperature in the sea ice brine at tested temperatures and were capable of biotransforming hydrocarbon compounds. Complete depletion of low and middle-range molecular weight n-alkanes, 2/3 ring PAHs and their alkylated compounds was observed after 123 days at -2°C. The depletion extents of hydrocarbon compounds were reduced at -6°C in comparison with -2°C, especially for PAHs and alkylated hydrocarbons. This study suggests that: there is a potential for biodegradation of dispersed oil in sea ice brine at temperatures between -2°C and -6°C. However, for oil with high pour point, the biodegradation process will be more affected by low temperature. Therefore, to predict the fate of dispersed oil at low temperature, the biodegradation rate should be established for each individual oil type and at a specific temperature.
