RESUMO
The paper contains a parametric analysis of tensegrity structures subjected to periodic loads. The analysis focuses on determining the main region of dynamic instability. When load parameters fall within this region, the resulting vibration amplitudes increase, posing a risk to the durability of structures. The study considers structures built using commonly used modules. The influence of the initial prestress on the distribution of the instability regions is examined. Additional prestress can significantly reduce the extent of instability regions, potentially narrowing them by up to 99%. A nondimensional parameter is introduced to accurately assess changes in the extent of the instability region. A geometrically non-linear model is employed to evaluate the behavior of the analyzed structures.
RESUMO
The paper contains a parametric analysis of tensegrity structures subjected to time-independent external loads. A complete dynamic stability analysis is a three-step process. The first stage involves the identification of self-stress states and infinitesimal mechanisms. The next stage concentrates on the static and dynamic behavior of tensegrities under time-independent external loads, whereas the third is under periodic loads. In this paper, the first two stages are carried out. The structures built with the most popular tensegrity modules, Simplex and Quartex, are considered. The effect of the initial prestress on the static parameters and frequency is analyzed. To assess this behavior, a geometrically non-linear model is used.
RESUMO
The aim of this study is to prove that it is possible to control the static behavior of tensegrity plate-like structures. This possibility is very important, particularly in the case of deployable structures. Here, we analyze the impact the support conditions of the structure have on the existence of specific characteristics, such as self-stress states and infinitesimal mechanisms, and, consequently, on the active control. Plates built with Simplex modules are considered. Firstly, the presence of the specific characteristics is examined, and a classification is carried out. Next, the influence of the level of self-stress state on the behavior of structures is analyzed. A geometrically non-linear model, implemented in an original program, written in the Mathematica environment, is used. The results confirm the feasibility of the active control of stiffness of tensegrity plate-like structures characterized by the presence of infinitesimal mechanisms. In the case when mechanisms do not exist, structures are insensitive to the initial prestress level. It is possible to control the occurrence of mechanisms by changing the support conditions of the structure. Based on the obtained results, tensegrity is very promising structural concept, applicable in many areas, when conventional solutions are insufficient.
RESUMO
BACKGROUND: The enhanceosome is an enhancer located upstream of the human interferon ß gene, bound by transcription factor (TF) complex of extremely rigid structure. Within these rigid constraints, even a slight change of distances between transcription factor binding sites (TFBS) results in loss of functionality of the enhanceosome. We hypothesized that smaller subunits of the enhanceosome may entail TF complex formation in other regulatory regions. RESULTS: In order to verify this hypothesis we systematically searched for dimerization preferences of the TFs that have TFBS in the enhanceosome. For this we utilized our recently developed tool, TACO. We performed this computational experiment in a cell-type-specific manner by utilizing cell-type-specific DNase-seq data for 105 human cell types. We also used 20 TRANSFAC motifs comprising not only the usual TFs constituting the enhanceosome but also the architectural proteins of High Mobility Group I(Y) (HMG I). A similar experiment used 42 DNase-seq data sets for mouse cell types. We found 137 statistically significant dimer predictions in the human genome, and 37 predictions in the mouse genome, that matched the positioning on the enhanceosome with ±2 bp tolerance. To characterize these predicted TF dimers, we performed functional analysis (Gene Ontology enrichment) for sets of genes which were in the neighbourhood of predicted dimer instances. A notable feature of these instances is that (1) most of them are located in introns of genes, (2) they are enriched in regulatory states, and (3) those instances that are located near transcription start sites are enriched for inclusion in computationally predicted enhancers. We also investigated similarity of dimer predictions between human and mouse. CONCLUSIONS: It follows from our experiments that, except for homodimer formed by IRF proteins, the rest of the dimers were formed exclusively between one of the transcriptional activators (ATF-2/c-Jun and IRF) and a HMG I protein. NF- κB did not participate in forming dimers with other proteins. Dimers predicted in mouse were fully contained in those predicted in human, with exactly the same spacing and orientation. Intriguingly, in most of the cases the enhanceosome motifs have 1 bp wider spacing than the corresponding dimers predicted genome-wide, which is likely caused by the overall 3D structure constraints of the enhanceosome-bound complex.
